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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A7G6




SWISS-2DPAGE:  P0A7G6


P0A7G6


General information about the entry
View entry in simple text format
Entry nameRECA_ECOLI
Primary accession numberP0A7G6
Secondary accession number(s) P03017
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=Protein RecA; AltName: Full=Recombinase A;.
Gene nameName=recA
Synonyms=lexB, recH, rnmB, tif, umuB, zab
OrderedLocusNames=b2699, JW2669
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   PROTEIN EXPRESSION
MEDLINE=87194592; PubMed=3553157; [NCBI, ExPASy, EBI, Israel, Japan]
Jones P.G., Vanbogelen R.A., Neidhardt F.C.
''''''Induction of proteins in response to low temperature in Escherichia coli'';'';''
J. Bacteriol. 169(1):2092-2095(1987)
[4]   PROTEIN EXPRESSION
MEDLINE=87083376; PubMed=3539918; [NCBI, ExPASy, EBI, Israel, Japan]
Vanbogelen R.A., Kelley P.M., Neidhardt F.C.
''''''Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli'';'';''
J. Bacteriol. 169(1):26-32(1987)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.08; Mw=41219

EXPRESSION:
INCREASED DURING THE ADJUSTMENT OF E. COLI TO GROWTH AT 10°C [3]; WAS LARGELY INDUCED BY CADMIUM CHLORIDE, HYDROGEN PEROXIDE AND ACDQ [4].

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
ECO2DBASEC039.3; 6TH EDITION.
UniProtKB/Swiss-ProtP0A7G6; RECA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRECA_ECOLI
Primary accession numberP0A7G6
Secondary accession number(s) P03017 P26347 P78213
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 67)
Name and origin of the protein
DescriptionRecName: Full=Protein RecA; AltName: Full=Recombinase A;
Gene nameName=recA
Synonyms=lexB, recH, rnmB, tif, umuB, zab
OrderedLocusNames=b2699, JW2669
Encoded onName=recA; Synonyms=lexB, recH, rnmB, tif, umuB, zab; OrderedLocusNames=b2699, JW2669
Keywords3D-structure; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; DNA-binding; Nucleotide-binding; Reference proteome; SOS response; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00328; CAA23618.1; -; Genomic_DNA
EMBLU00096; AAC75741.1; -; Genomic_DNA
EMBLAP009048; BAA16561.2; -; Genomic_DNA
PIRG65049; RQECA; .
RefSeqNP_417179.1; NC_000913.2; .
PDB1AA3; NMR; -; A=269-330
PDB1N03; EM; 20.00 A; A/B/C/D/E/F/G=2-352
PDB1REA; X-ray; 2.70 A; A=2-353
PDB1U94; X-ray; 1.90 A; A=2-352
PDB1U98; X-ray; 2.00 A; A=2-352
PDB1U99; X-ray; 2.60 A; A=2-352
PDB1XMS; X-ray; 2.10 A; A=2-352
PDB1XMV; X-ray; 1.90 A; A=2-352
PDB2REB; X-ray; 2.30 A; A=2-353
PDB2REC; EM; -; A/B/C/D/E/F=1-353
PDB3CMT; X-ray; 3.15 A; A/D=1-335
PDB3CMU; X-ray; 4.20 A; A=1-335
PDB3CMV; X-ray; 4.30 A; A/B/C/D/E/F/G/H=1-335
PDB3CMW; X-ray; 2.80 A; A/C=1-335
PDB3CMX; X-ray; 3.40 A; A/D=1-335
PDBsum1AA3; -; .
PDBsum1N03; -; .
PDBsum1REA; -; .
PDBsum1U94; -; .
PDBsum1U98; -; .
PDBsum1U99; -; .
PDBsum1XMS; -; .
PDBsum1XMV; -; .
PDBsum2REB; -; .
PDBsum2REC; -; .
PDBsum3CMT; -; .
PDBsum3CMU; -; .
PDBsum3CMV; -; .
PDBsum3CMW; -; .
PDBsum3CMX; -; .
ProteinModelPortalP0A7G6; -; .
SMRP0A7G6; 7-329; .
DIPDIP-31832N; -; .
IntActP0A7G6; 26; .
MINTMINT-1300726; -; .
SWISS-2DPAGEP0A7G6; -; .
ECO2DBASEC039.3; 6TH EDITION; .
EnsemblBacteriaEBESCT00000004487; EBESCP00000004487; EBESCG00000003663; .
EnsemblBacteriaEBESCT00000004488; EBESCP00000004488; EBESCG00000003663; .
EnsemblBacteriaEBESCT00000017937; EBESCP00000017228; EBESCG00000016993; .
GeneID947170; -; .
GenomeReviewsAP009048_GR; JW2669; .
GenomeReviewsU00096_GR; b2699; .
KEGGecj:JW2669; -; .
KEGGeco:b2699; -; .
EchoBASEEB0816; -; .
EcoGeneEG10823; recA; .
eggNOGCOG0468; -; .
GeneTreeEBGT00050000009797; -; .
HOGENOMHBG339889; -; .
OMATRKGAWY; -; .
ProtClustDBPRK09354; -; .
BioCycEcoCyc:EG10823-MONOMER; -; .
GenevestigatorP0A7G6; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0008094; F:DNA-dependent ATPase activity; IEA:InterPro; .
GOGO:0003697; F:single-stranded DNA binding; IDA:EcoliWiki; .
GOGO:0048870; P:cell motility; IMP:EcoliWiki; .
GOGO:0006310; P:DNA recombination; IEA:UniProtKB-KW; .
GOGO:0006281; P:DNA repair; IEA:UniProtKB-KW; .
GOGO:0009432; P:SOS response; IEA:UniProtKB-KW; .
HAMAPMF_00268; RecA; 1; -
InterProIPR003593; ATPase_AAA+_core; .
InterProIPR013765; DNA_recomb/repair_RecA; .
InterProIPR020584; DNA_recomb/repair_RecA_CS; .
InterProIPR020588; DNA_recomb_RecA/RadB_ATP-bd; .
InterProIPR023400; RecA_C; .
InterProIPR020587; RecA_monomer-monomer_interface; .
Gene3DG3DSA:3.30.250.10; G3DSA:3.30.250.10; 1; .
PANTHERPTHR22942:SF1; RecA; 1; .
PfamPF00154; RecA; 1; .
PRINTSPR00142; RECA; .
SMARTSM00382; AAA; 1; .
SUPFAMSSF54752; SSF54752; 1; .
TIGRFAMsTIGR02012; Tigrfam_recA; 1; .
PROSITEPS00321; RECA_1; 1; .
PROSITEPS50162; RECA_2; 1; .
PROSITEPS50163; RECA_3; 1; .



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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server