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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0C0L5




SWISS-2DPAGE:  P0C0L5


P0C0L5


General information about the entry
View entry in simple text format
Entry nameCO4B_HUMAN
Primary accession numberP0C0L5
Secondary accession number(s) P01028
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onDecember 1, 2000 (version 1)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Complement C4-B; AltName: Full=Basic complement C4; AltName: Full=C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; Contains: RecName: Full=Complement C4 beta chain; Contains: RecName: Full=Complement C4-B alpha chain; Contains: RecName: Full=C4a anaphylatoxin; Contains: RecName: Full=C4b-B; Contains: RecName: Full=C4d-B; Contains: RecName: Full=Complement C4 gamma chain; Flags: Precursor;.
Gene nameName=C4B
Synonyms=CO4, CPAMD3
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93092937; PubMed=1459097; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez J.-C., James R.W., Tissot J.-D., Bjellqvist B., Hochstrasser D.F.
''''''Plasma protein map: an update by microsequencing'';'';''
Electrophoresis 13(1):707-714(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
Comments
  • SUBUNIT: ALPHA, BETA AND GAMMA CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=6.39; Mw=30097
pI=6.54; Mw=29949

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=6.78; Mw=27155
pI=6.52; Mw=27030

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=6.41; Mw=31942
pI=6.54; Mw=31735

NORMAL LEVEL:
200-500 MG/L.

PATHOLOGICAL LEVEL:
DECREASED DURING LUPUS ERYTHEMATOSUS AND HEREDITARY ANGIOEDEMA.

POSITIONAL VARIANTS:
MORE THAN 10 VARIANTS.

MAPPING (identification):
MICROSEQUENCING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS-2DPAGEP0C0L5; CO4B_HUMAN.
UniProtKB/Swiss-ProtP0C0L5; CO4B_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCO4B_HUMAN
Primary accession numberP0C0L5
Secondary accession number(s) P01028 P78445 Q13160 Q13906 Q14033 Q14835 Q9NPK5 Q9UIP5
Sequence was last modified on November 8, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 72)
Name and origin of the protein
DescriptionRecName: Full=Complement C4-B; AltName: Full=Basic complement C4; AltName: Full=C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; Contains: RecName: Full=Complement C4 beta chain; Contains: RecName: Full=Complement C4-B alpha chain; Contains: RecName: Full=C4a anaphylatoxin; Contains: RecName: Full=C4b-B; Contains: RecName: Full=C4d-B; Contains: RecName: Full=Complement C4 gamma chain; Flags: Precursor;
Gene nameName=C4B
Synonyms=CO4, CPAMD3
Encoded onName=C4B; Synonyms=CO4, CPAMD3
KeywordsBlood group antigen; Cleavage on pair of basic residues; Complement pathway; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunity; Inflammatory response; Innate immunity; Polymorphism; Reference proteome; Secreted; Signal; Sulfation; Systemic lupus erythematosus; Thioester bond.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU24578; AAA99717.1; ALT_SEQ; Genomic_DNA
EMBLAF019413; AAB67980.1; -; Genomic_DNA
EMBLAL049547; CAB89302.1; -; Genomic_DNA
EMBLK02404; AAA59651.1; -; mRNA
IPIIPI00654875; -; .
PIRB20807; B20807; .
RefSeqNP_001002029.3; NM_001002029.3; .
RefSeqNP_001229752.1; NM_001242823.2; .
UniGeneHs.534847; -; .
UniGeneHs.720022; -; .
ProteinModelPortalP0C0L5; -; .
SMRP0C0L5; 138-239; 683-745; 764-984
996-13211324-1743; .
IntActP0C0L5; 1; .
STRINGP0C0L5; -; .
MEROPSI39.951; -; .
PhosphoSiteP0C0L5; -; .
SWISS-2DPAGEP0C0L5; -; .
PRIDEP0C0L5; -; .
EnsemblENST00000414246; ENSP00000403377; ENSG00000236625; .
EnsemblENST00000449788; ENSP00000414200; ENSG00000236625; .
GeneID100293534; -; .
GeneID721; -; .
KEGGhsa:721; -; .
CTD721; -; .
GeneCardsGC06P031736; -; .
GeneCardsGC06Po31871; -; .
HGNCHGNC:1324; C4B; .
MIM120790; phenotype; .
MIM120820; gene; .
MIM152700; phenotype; .
neXtProtNX_P0C0L5; -; .
Orphanet169147; Immunodeficiency due to an early component of complement deficiency; .
HOVERGENHBG107123; -; .
InParanoidP0C0L5; -; .
OrthoDBEOG4JM7NW; -; .
ReactomeREACT_6900; Immune System; .
GenevestigatorP0C0L5; -; .
GermOnlineENSG00000204319; Homo sapiens; .
GOGO:0005615; C:extracellular space; IEA:InterPro; .
GOGO:0004866; F:endopeptidase inhibitor activity; IEA:InterPro; .
GOGO:0006958; P:complement activation; classical pathway; IEA:UniProtKB-KW
GOGO:0006954; P:inflammatory response; IEA:UniProtKB-KW; .
GOGO:0045087; P:innate immune response; TAS:Reactome; .
InterProIPR009048; A-macroglobulin_rcpt-bd; .
InterProIPR011626; A2M_comp; .
InterProIPR002890; A2M_N; .
InterProIPR011625; A2M_N_2; .
InterProIPR000020; Anaphylatoxin/fibulin; .
InterProIPR018081; Anaphylatoxin_; .
InterProIPR001840; Anaphylatoxn; .
InterProIPR001599; Macroglobln_a2; .
InterProIPR019742; MacrogloblnA2_CS; .
InterProIPR019565; MacrogloblnA2_thiol-ester-bond; .
InterProIPR001134; Netrin_domain; .
InterProIPR018933; Netrin_module_non-TIMP; .
InterProIPR008930; Terpenoid_cyclase/PrenylTrfase; .
InterProIPR008993; TIMP-like_OB-fold; .
Gene3DG3DSA:2.60.40.690; A-macroglobulin_rcpt-bd; 1; .
Gene3DG3DSA:1.20.91.20; Anaphylatoxin; 1; .
PfamPF00207; A2M; 1; .
PfamPF07678; A2M_comp; 1; .
PfamPF01835; A2M_N; 1; .
PfamPF07703; A2M_N_2; 1; .
PfamPF07677; A2M_recep; 1; .
PfamPF01821; ANATO; 1; .
PfamPF01759; NTR; 1; .
PfamPF10569; Thiol-ester_cl; 1; .
PRINTSPR00004; ANAPHYLATOXN; .
SMARTSM00104; ANATO; 1; .
SMARTSM00643; C345C; 1; .
SUPFAMSSF49410; AM_receptor_bind; 1; .
SUPFAMSSF47686; Anaphylatoxin; 1; .
SUPFAMSSF48239; Terp_cyc_toroid; 1; .
SUPFAMSSF50242; TIMP_like; 1; .
PROSITEPS00477; ALPHA_2_MACROGLOBULIN; 1; .
PROSITEPS01177; ANAPHYLATOXIN_1; 1; .
PROSITEPS01178; ANAPHYLATOXIN_2; 1; .
PROSITEPS50189; NTR; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server