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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P10809




SWISS-2DPAGE:  P10809


P10809


General information about the entry
View entry in simple text format
Entry nameCH60_HUMAN
Primary accession numberP10809
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=HuCHA60; AltName: Full=Mitochondrial matrix protein P1; AltName: Full=P60 lymphocyte protein; Flags: Precursor;.
Gene nameName=HSPD1
Synonyms=HSP60
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[4]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[5]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[6]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[7]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[8]   MAPPING ON GEL
PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
Comments
  • SUBUNIT: SINGLE CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=5.04; Mw=56704
pI=5.16; Mw=56244
pI=5.09; Mw=56244

MAPPING (identification):
MICROSEQUENCING [6].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=5.16; Mw=56190
pI=5.11; Mw=56479
pI=5.07; Mw=56917
pI=5.21; Mw=56625

MAPPING (identification):
MATCHING WITH THE HEPG2 MASTER GEL [7].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=5.23; Mw=59505
pI=5.28; Mw=59403
pI=5.35; Mw=59302

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=5.04; Mw=58262
pI=5.09; Mw=58000
pI=5.14; Mw=57838
pI=5.19; Mw=57516
pI=5.24; Mw=57516

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=5.25; Mw=57962

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4][5].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=5.11; Mw=56186  [identification data]
pI=5.23; Mw=55929
pI=5.14; Mw=56057

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL, AMINO ACID COMPOSITION ANALYSIS AND IMMUNODETECTION [3].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.12; Mw=57802
pI=5.18; Mw=57962
pI=5.24; Mw=57962

MAPPING (identification):
MICROSEQUENCING (AKDVKFGAD) [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=5.13; Mw=56842
pI=5.18; Mw=56842
pI=5.25; Mw=56710

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



NUCLEOLI_HELA_2D_HUMAN {2D-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_2D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_2D_HUMAN

MAP LOCATIONS:
pI=5.50; Mw=60991  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [8].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP10809; P10809.
DOSAC-COBS-2DPAGEP10809; CH60_HUMAN.
HSC-2DPAGEP10809; HUMAN.
OGP-WWWP10809.
PHCI-2DPAGEP10809.
PMMA-2DPAGEP10809.
REPRODUCTION-2DPAGEP10809; P10809.
REPRODUCTION-2DPAGEIPI00784154; IPI00784154.
Siena-2DPAGEP10809; CH60_HUMAN.
UCD-2DPAGEP10809; CH60_HUMAN.
UniProtKB/Swiss-ProtP10809; CH60_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCH60_HUMAN
Primary accession numberP10809
Secondary accession number(s) B2R5M6 Q38L19 Q9UCR6
Sequence was last modified on August 1, 1990 (version 2)
Annotations were last modified on October 19, 2011 (version 148)
Name and origin of the protein
DescriptionRecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=HuCHA60; AltName: Full=Mitochondrial matrix protein P1; AltName: Full=P60 lymphocyte protein; Flags: Precursor;
Gene nameName=HSPD1
Synonyms=HSP60
Encoded onName=HSPD1; Synonyms=HSP60
KeywordsAcetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Disease mutation; Hereditary spastic paraplegia; Host-virus interaction; Leukodystrophy; Mitochondrion; Neurodegeneration; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM22382; AAA60127.1; -; mRNA
EMBLM34664; AAA36022.1; -; mRNA
EMBLAJ250915; CAB75426.1; -; Genomic_DNA
EMBLDQ217936; ABB01006.1; -; Genomic_DNA
EMBLAK312240; BAG35173.1; -; mRNA
EMBLBC002676; AAH02676.1; -; mRNA
EMBLBC003030; AAH03030.1; -; mRNA
EMBLBC067082; AAH67082.1; -; mRNA
EMBLBC073746; AAH73746.1; -; mRNA
IPIIPI00784154; -; .
PIRA32800; A32800; .
RefSeqNP_002147.2; NM_002156.4; .
RefSeqNP_955472.1; NM_199440.1; .
UniGeneHs.595053; -; .
UniGeneHs.727543; -; .
ProteinModelPortalP10809; -; .
SMRP10809; 26-551; .
DIPDIP-58N; -; .
IntActP10809; 32; .
MINTMINT-1162735; -; .
STRINGP10809; -; .
PhosphoSiteP10809; -; .
SWISS-2DPAGEP10809; -; .
DOSAC-COBS-2DPAGEP10809; -; .
OGPP10809; -; .
PHCI-2DPAGEP10809; -; .
PMMA-2DPAGEP10809; -; .
REPRODUCTION-2DPAGEIPI00784154; -; .
REPRODUCTION-2DPAGEP10809; -; .
Siena-2DPAGEP10809; -; .
UCD-2DPAGEP10809; -; .
PRIDEP10809; -; .
EnsemblENST00000345042; ENSP00000340019; ENSG00000144381; .
EnsemblENST00000388968; ENSP00000373620; ENSG00000144381; .
GeneID3329; -; .
KEGGhsa:3329; -; .
UCSCuc002uui.1; human; .
CTD3329; -; .
GeneCardsGC02M190203; -; .
H-InvDBHIX0002706; -; .
H-InvDBHIX0032069; -; .
HGNCHGNC:5261; HSPD1; .
HPACAB002775; -; .
HPAHPA001523; -; .
MIM118190; gene; .
MIM605280; phenotype; .
MIM612233; phenotype; .
neXtProtNX_P10809; -; .
Orphanet100994; Autosomal dominant spastic paraplegia type 13; .
Orphanet702; Pelizaeus-Merzbacher disease; .
PharmGKBPA29527; -; .
eggNOGprNOG10365; -; .
HOGENOMHBG625289; -; .
HOVERGENHBG001982; -; .
InParanoidP10809; -; .
OMAVQSIIPA; -; .
OrthoDBEOG4PK27J; -; .
PhylomeDBP10809; -; .
NextBio13188; -; .
ArrayExpressP10809; -; .
BgeeP10809; -; .
CleanExHS_HSPD1; -; .
GenevestigatorP10809; -; .
GermOnlineENSG00000144381; Homo sapiens; .
GOGO:0009986; C:cell surface; IDA:UniProtKB; .
GOGO:0005905; C:coated pit; IDA:BHF-UCL; .
GOGO:0030135; C:coated vesicle; IDA:BHF-UCL; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005769; C:early endosome; IDA:BHF-UCL; .
GOGO:0005615; C:extracellular space; IDA:BHF-UCL; .
GOGO:0046696; C:lipopolysaccharide receptor complex; IDA:BHF-UCL; .
GOGO:0005743; C:mitochondrial inner membrane; ISS:BHF-UCL; .
GOGO:0005759; C:mitochondrial matrix; TAS:BHF-UCL; .
GOGO:0030141; C:stored secretory granule; ISS:BHF-UCL; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0016887; F:ATPase activity; ISS:BHF-UCL; .
GOGO:0043498; F:cell surface binding; IDA:BHF-UCL; .
GOGO:0051087; F:chaperone binding; IPI:UniProtKB; .
GOGO:0003688; F:DNA replication origin binding; ISS:BHF-UCL; .
GOGO:0001530; F:lipopolysaccharide binding; IDA:BHF-UCL; .
GOGO:0002039; F:p53 binding; IPI:UniProtKB; .
GOGO:0003697; F:single-stranded DNA binding; ISS:BHF-UCL; .
GOGO:0051082; F:unfolded protein binding; IC:UniProtKB; .
GOGO:0006458; P:'de novo' protein folding; ISS:BHF-UCL; .
GOGO:0006919; P:activation of caspase activity; IDA:BHF-UCL; .
GOGO:0002368; P:B cell cytokine production; IDA:BHF-UCL; .
GOGO:0042100; P:B cell proliferation; IDA:BHF-UCL; .
GOGO:0051131; P:chaperone-mediated protein complex assembly; ISS:BHF-UCL; .
GOGO:0044419; P:interspecies interaction between organisms; IEA:UniProtKB-KW; .
GOGO:0048291; P:isotype switching to IgG isotypes; IDA:BHF-UCL; .
GOGO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; IDA:BHF-UCL; .
GOGO:0043066; P:negative regulation of apoptosis; IMP:UniProtKB; .
GOGO:0043065; P:positive regulation of apoptosis; IMP:BHF-UCL; .
GOGO:0032727; P:positive regulation of interferon-alpha production; IDA:BHF-UCL; .
GOGO:0032729; P:positive regulation of interferon-gamma production; IDA:BHF-UCL; .
GOGO:0032733; P:positive regulation of interleukin-10 production; IDA:BHF-UCL; .
GOGO:0032735; P:positive regulation of interleukin-12 production; IDA:BHF-UCL; .
GOGO:0032755; P:positive regulation of interleukin-6 production; IDA:BHF-UCL; .
GOGO:0043032; P:positive regulation of macrophage activation; IDA:BHF-UCL; .
GOGO:0050870; P:positive regulation of T cell activation; IDA:BHF-UCL; .
GOGO:0002842; P:positive regulation of T cell mediated immune response to tumor cell; IDA:BHF-UCL; .
GOGO:0051604; P:protein maturation; ISS:BHF-UCL; .
GOGO:0042026; P:protein refolding; IDA:UniProtKB; .
GOGO:0050821; P:protein stabilization; IMP:UniProtKB; .
GOGO:0006986; P:response to unfolded protein; IDA:BHF-UCL; .
GOGO:0042110; P:T cell activation; IDA:MGI; .
InterProIPR018370; Chaperonin_Cpn60_CS; .
InterProIPR001844; Chaprnin_Cpn60; .
InterProIPR002423; Cpn60/TCP-1; .
PANTHERPTHR11353; Cpn60/TCP-1; 1; .
PfamPF00118; Cpn60_TCP1; 1; .
PRINTSPR00298; CHAPERONIN60; .
SUPFAMSSF48592; GroEL-ATPase; 1; .
TIGRFAMsTIGR02348; GroEL; 1; .
PROSITEPS00296; CHAPERONINS_CPN60; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server