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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: Q9H9Y6




SWISS-2DPAGE:  Q9H9Y6


Q9H9Y6


General information about the entry
View entry in simple text format
Entry nameRPA2_HUMAN
Primary accession numberQ9H9Y6
integrated into SWISS-2DPAGE on November 9, 2001 (release 15)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=DNA-directed RNA polymerase I subunit RPA2; Short=RNA polymerase I subunit 2; EC=2.7.7.6; AltName: Full=DNA-directed RNA polymerase I 135 kDa polypeptide; Short=RPA135;.
Gene nameName=POLR1B
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
2D PAGE maps for identified proteins
How to interpret a protein

NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_1D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_1D_HUMAN

MAP LOCATIONS:
Mw=105656  [identification data]

MAPPING (identification):
Tandem mass spectrometry [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtQ9H9Y6; RPA2_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRPA2_HUMAN
Primary accession numberQ9H9Y6
Secondary accession number(s) Q585T5 Q6ZRR2 Q9H9D3
Sequence was last modified on April 4, 2006 (version 2)
Annotations were last modified on October 19, 2011 (version 98)
Name and origin of the protein
DescriptionRecName: Full=DNA-directed RNA polymerase I subunit RPA2; Short=RNA polymerase I subunit 2; EC=2.7.7.6; AltName: Full=DNA-directed RNA polymerase I 135 kDa polypeptide; Short=RPA135;
Gene nameName=POLR1B
Encoded onName=POLR1B
KeywordsAlternative splicing; Complete proteome; DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase; Nucleus; Polymorphism; Reference proteome; Transcription; Transferase; Zinc; Zinc-finger.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAK022533; BAB14082.1; -; mRNA
EMBLAK022890; BAB14296.1; -; mRNA
EMBLAK128044; BAC87247.1; -; mRNA
EMBLAC012442; AAX81999.1; -; Genomic_DNA
IPIIPI00026445; -; .
IPIIPI01010348; -; .
RefSeqNP_001131076.1; NM_001137604.1; .
RefSeqNP_061887.2; NM_019014.4; .
UniGeneHs.726599; -; .
ProteinModelPortalQ9H9Y6; -; .
SMRQ9H9Y6; 656-698; .
DIPDIP-27538N; -; .
IntActQ9H9Y6; 10; .
MINTMINT-4727878; -; .
STRINGQ9H9Y6; -; .
PhosphoSiteQ9H9Y6; -; .
SWISS-2DPAGEQ9H9Y6; -; .
PRIDEQ9H9Y6; -; .
EnsemblENST00000263331; ENSP00000263331; ENSG00000125630; .
GeneID84172; -; .
KEGGhsa:84172; -; .
NMPDRfig|9606.3.peg.18493; -; .
UCSCuc002thw.1; human; .
CTD84172; -; .
GeneCardsGC02P105754; -; .
HGNCHGNC:20454; POLR1B; .
HPAHPA037528; -; .
MIM602000; gene; .
neXtProtNX_Q9H9Y6; -; .
PharmGKBPA130601182; -; .
HOVERGENHBG017742; -; .
InParanoidQ9H9Y6; -; .
OMASHFRAIH; -; .
OrthoDBEOG4H9XJP; -; .
PhylomeDBQ9H9Y6; -; .
ReactomeREACT_1788; Transcription; .
NextBio73536; -; .
ArrayExpressQ9H9Y6; -; .
BgeeQ9H9Y6; -; .
CleanExHS_POLR1B; -; .
GenevestigatorQ9H9Y6; -; .
GermOnlineENSG00000125630; Homo sapiens; .
GOGO:0005654; C:nucleoplasm; TAS:Reactome; .
GOGO:0003677; F:DNA binding; IEA:InterPro; .
GOGO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0032549; F:ribonucleoside binding; IEA:InterPro; .
GOGO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome; .
GOGO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome; .
GOGO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome; .
InterProIPR015712; DNA-dir_RNA_pol_su2; .
InterProIPR007120; DNA-dir_RNA_pol_su2_6; .
InterProIPR007121; RNA_pol_bsu_CS; .
InterProIPR007644; RNA_pol_bsu_protrusion; .
InterProIPR009674; RNA_pol_Rpa2-specific; .
InterProIPR007642; RNA_pol_Rpb2_2; .
InterProIPR007645; RNA_pol_Rpb2_3; .
InterProIPR007647; RNA_pol_Rpb2_5; .
InterProIPR007641; RNA_pol_Rpb2_7; .
Gene3DG3DSA:2.40.270.10; G3DSA:2.40.270.10; 2; .
PANTHERPTHR20856; RNA_pol_I_sub2; 1; .
PfamPF06883; RNA_pol_Rpa2_4; 1; .
PfamPF04563; RNA_pol_Rpb2_1; 1; .
PfamPF04561; RNA_pol_Rpb2_2; 1; .
PfamPF04565; RNA_pol_Rpb2_3; 1; .
PfamPF04567; RNA_pol_Rpb2_5; 1; .
PfamPF00562; RNA_pol_Rpb2_6; 1; .
PfamPF04560; RNA_pol_Rpb2_7; 1; .
PROSITEPS01166; RNA_POL_BETA; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server