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Differential Protein
Expression Analysis

HCP Antibody
Coverage Analysis

Differential Protein Expression Analysis HCP Antibody Coverage Analysis


The Proteome Imaging group, affiliated to the Swiss-Prot Group of SIB Swiss Institute of Bioinformatics has been developing Melanie since more than three decades rendering this two-dimensional electrophoresis (2-DE) gel analysis platform one of today's market leaders for analyzing, annotating and querying complex 2-D gel samples.

The latest versions of Melanie were developed to replace the DeCyder™ 2D and ImageMaster™ 2D Platinum applications, with a single goal in mind: help you draw more reliable conclusions from all your 2-D electrophoresis data. With its intuitive workflow and its 100% matching algorithm, analyzing your protein experiment has never been so easy.

The most recent product releases are Melanie 9 enabling our users to seamlessly perform both differential protein expression analysis (for single stain and DIGE gels) but also include a dedicated workflow for analysis of Host Cell Protein (HCP) antibody Coverage analysis. These versions provide powerful and innovative solutions to shorten the path from data acquisition to protein information. The superior spot detection and matching algorithms facilitate the extraction of statistically valid differences between groups of 2-D gels, while requiring minimal user intervention and therefore speeding up analysis time. The application integrates filtering, querying, reporting, statistical and graphing options so that you can easily view, compare, analyze and present your results.

Melanie is jointly distributed by SIB Swiss Institute of Bioinformatics and by Cytiva (formerly GE Healthcare Life Sciences), offering to scientists a worldwide network specialists helping them using our software wherever they are and give us a unique way to get user's day-to-day feedback to enhanced our solutions.

For more information, benefits, features, quotes, documentation and a free demo version, we invite you to visit

Non DIGE image analysis

Expression analysis

Host cell protein analysis

Host cell protein analysis

Melanie DIGE&HCP
Workflow Uncertain Review
Diagrams Quadrant Formula

Melanie Coverage
Workflow Automatic Detection Experimental Design
Alignment Automatic Detection Experimental Design

Free Viewer

Useful tools to the international scientific community. The Melanie 9 trial version is also a viewer available free of charge to help researchers visualize gels and related data obtained through the use of the full version of Melanie by colleagues.

The viewer includes similar functionalities as the full versions of the software with the following limitations: Spots and matches can only be created or edited on the tutorials gels.

For more information, benefits, features, quotes and documentation, we invite you to visit

Try Melanie

Our Mascot

Melanie has been developed in Geneva, at the foot of the Swiss Alps, where the Saint Bernard dog is at home. Barry has thus been adopted as Melanie's mascot. Visit our mascot page.