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Searching in 'World-2DPAGE Repository [0003]' for entry matching: P48491




World-2DPAGE Repository (0003):  P48491


P48491


General information about the entry
View entry in simple text format
Entry nameTPIS_ARATH
Primary accession numberP48491
Secondary accession number(s) Q9M2T5
integrated into World-2DPAGE Repository (0003) on October 14, 2008 (release 1)
2D Annotations were last modified onMay 18, 2011 (version 2)
General Annotations were last modified on November 15, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Triosephosphate isomerase, cytosolic; Short=TIM; Short=Triose-phosphate isomerase; EC=5.3.1.1;.
Gene nameName=CTIMC
OrderedLocusNames=At3g55440
ORFNames=T22E16.100
Annotated speciesArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
TaxonomyEukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core; eudicotyledons; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
References
[1]   MAPPING ON GEL
PubMed=18656559; DOI=10.1016/j.jprot.2008.06.012; [NCBI, EBI, Israel, Japan]
Maldonado A.M., Echevarria-Zomeno S., Jean-Baptiste S., Hernandez M., Jorrin-Novo J.V.
''Evaluation of three different protocols of protein extraction for Arabidopsis thaliana leaf proteome analysis by two-dimensional electrophoresis''
J. Proteomics 71(4):461-472 (2008)
2D PAGE maps for identified proteins
How to interpret a protein

PHENOL_2 {Phenol 2}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
PHENOL_2
  map experimental info
 
PHENOL_2

MAP LOCATIONS:
pI=6.17; Mw=30130  [identification data]
pI=6.60; Mw=18210  [identification data]

IDENTIFICATION: SPOT 52: SeqCov=38%. Mascot (Protein score/CI%=176/100, Total ion score/CI%=73/100) [1]
SPOT 63: SeqCov=40%. Mascot (Protein score/CI%=119/100) [1].
MAPPING (identification):
SPOT 52: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 63: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].



TCA-ACETONE_3 {TCA-Acetone 3}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
TCA-ACETONE_3
  map experimental info
 
TCA-ACETONE_3

MAP LOCATIONS:
pI=6.17; Mw=30130  [identification data]
pI=6.60; Mw=18210  [identification data]

IDENTIFICATION: SPOT 52: SeqCov=38%. Mascot (Protein score/CI%=176/100, Total ion score/CI%=73/100) [1]
SPOT 63: SeqCov=40%. Mascot (Protein score/CI%=119/100) [1].
MAPPING (identification):
SPOT 52: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 63: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].



TCA-ACETONE-PHENOL_3 {TCA-Acetone-Phenol 3}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
TCA-ACETONE-PHENOL_3
  map experimental info
 
TCA-ACETONE-PHENOL_3

MAP LOCATIONS:
pI=6.17; Mw=30130  [identification data]
pI=6.60; Mw=18210  [identification data]

IDENTIFICATION: SPOT 52: SeqCov=38%. Mascot (Protein score/CI%=176/100, Total ion score/CI%=73/100) [1]
SPOT 63: SeqCov=40%. Mascot (Protein score/CI%=119/100) [1].
MAPPING (identification):
SPOT 52: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 63: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jesus V. Jorrin-Novo, University of Cordoba, Spain
Cross-references
SWISS-2DPAGEP48491; TPIS_ARATH.
UniProtKB/Swiss-ProtP48491; TPIS_ARATH.
World-2DPAGE RepositoryP48491; TPIS_ARATH.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTPIS_ARATH
Primary accession numberP48491
Secondary accession number(s) Q9M2T5
Sequence was last modified on April 27, 2001 (version 2)
Annotations were last modified on October 19, 2011 (version 90)
Name and origin of the protein
DescriptionRecName: Full=Triosephosphate isomerase, cytosolic; Short=TIM; Short=Triose-phosphate isomerase; EC=5.3.1.1;
Gene nameName=CTIMC
OrderedLocusNames=At3g55440
ORFNames=T22E16.100
Encoded onName=CTIMC; OrderedLocusNames=At3g55440; ORFNames=T22E16.100
KeywordsComplete proteome; Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase; Pentose shunt; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU02949; AAA03449.1; -; mRNA
EMBLAL132975; CAB75902.1; -; Genomic_DNA
EMBLCP002686; AEE79384.1; -; Genomic_DNA
EMBLAF375426; AAK53010.1; -; mRNA
EMBLAY074822; AAL69518.1; -; mRNA
IPIIPI00549121; -; .
PIRT47683; T47683; .
PIRT50646; T50646; .
RefSeqNP_191104.1; NM_115402.3; .
UniGeneAt.24563; -; .
UniGeneAt.31873; -; .
UniGeneAt.74726; -; .
ProteinModelPortalP48491; -; .
SMRP48491; 3-247; .
IntActP48491; 2; .
STRINGP48491; -; .
SWISS-2DPAGEP48491; -; .
World-2DPAGE0003:P48491; -; .
PRIDEP48491; -; .
ProMEXP48491; -; .
EnsemblPlantsAT3G55440.1; AT3G55440.1; AT3G55440; .
GeneID824710; -; .
GenomeReviewsBA000014_GR; AT3G55440; .
KEGGath:AT3G55440; -; .
NMPDRfig|3702.1.peg.16861; -; .
TAIRAt3g55440; -; .
eggNOGKOG1643; -; .
GeneTreeEPGT00070000029036; -; .
HOGENOMHBG708281; -; .
InParanoidP48491; -; .
OMAISGQWPR; -; .
PhylomeDBP48491; -; .
ProtClustDBPLN02561; -; .
BioCycARA:AT3G55440-MONOMER; -; .
BioCycMetaCyc:AT3G55440-MONOMER; -; .
ArrayExpressP48491; -; .
GenevestigatorP48491; -; .
GermOnlineAT3G55440; Arabidopsis thaliana; .
GOGO:0048046; C:apoplast; IDA:TAIR; .
GOGO:0005618; C:cell wall; IDA:TAIR; .
GOGO:0009570; C:chloroplast stroma; IDA:TAIR; .
GOGO:0005829; C:cytosol; IDA:TAIR; .
GOGO:0005739; C:mitochondrion; IDA:TAIR; .
GOGO:0005886; C:plasma membrane; IDA:TAIR; .
GOGO:0009506; C:plasmodesma; IDA:TAIR; .
GOGO:0005774; C:vacuolar membrane; IDA:TAIR; .
GOGO:0005507; F:copper ion binding; IDA:TAIR; .
GOGO:0004807; F:triose-phosphate isomerase activity; IDA:TAIR; .
GOGO:0006094; P:gluconeogenesis; IEA:UniProtKB-KW; .
GOGO:0006096; P:glycolysis; IC:TAIR; .
GOGO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-KW; .
GOGO:0046686; P:response to cadmium ion; IEP:TAIR; .
GOGO:0009651; P:response to salt stress; IEP:TAIR; .
GOGO:0010043; P:response to zinc ion; IEP:TAIR; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR022896; TrioseP_Isoase_bac/euk; .
InterProIPR000652; Triosephosphate_isomerase; .
InterProIPR020861; Triosephosphate_isomerase_AS; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
PANTHERPTHR21139; Triophos_ismrse; 1; .
PfamPF00121; TIM; 1; .
SUPFAMSSF51351; Triophos_ismrse; 1; .
TIGRFAMsTIGR00419; Tim; 1; .
PROSITEPS00171; TIM_1; 1; .
PROSITEPS51440; TIM_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server