resource logo

World-2DPAGE Repository

Attention: World-2DPAGE is no longer maintained.

World-2DPAGE Repository no longer accepts submissions.


World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Databases

use 'Ctrl' to select several
databases


Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal

Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0004]' for entry matching: P08461




World-2DPAGE Repository (0004):  P08461


P08461


General information about the entry
View entry in simple text format
Entry nameODP2_RAT
Primary accession numberP08461
integrated into World-2DPAGE Repository (0004) on October 13, 2008 (release 1)
2D Annotations were last modified onMay 18, 2011 (version 2)
General Annotations were last modified on December 22, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; EC=2.3.1.12; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor;.
Gene nameName=Dlat
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   MAPPING ON GEL
PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan]
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of the mature rat olfactory bulb''
Proteomics 9(1):2593-2599 (2009)
2D PAGE maps for identified proteins
How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb
OB21DSUB
  map experimental info
 
OB21DSUB

MAP LOCATIONS:
pI=5.62; Mw=63000  [identification data]

MAPPING (identification):
SPOT 243: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
UniProtKB/Swiss-ProtP08461; ODP2_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameODP2_RAT
Primary accession numberP08461
Secondary accession number(s) Q3B7V7
Sequence was last modified on December 12, 2006 (version 3)
Annotations were last modified on November 16, 2011 (version 119)
Name and origin of the protein
DescriptionRecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; EC=2.3.1.12; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor;
Gene nameName=Dlat
Encoded onName=Dlat
KeywordsAcetylation; Acyltransferase; Complete proteome; Direct protein sequencing; Glycolysis; Lipoyl; Mitochondrion; Reference proteome; Repeat; Transferase; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBC107440; AAI07441.1; -; mRNA
EMBLD10655; BAA01504.1; -; mRNA
EMBLD00092; BAA20956.1; -; mRNA
EMBLM16075; AAA41813.1; -; mRNA
IPIIPI00231714; -; .
PIRS21766; S21766; .
RefSeqNP_112287.1; NM_031025.1; .
UniGeneRn.15413; -; .
ProteinModelPortalP08461; -; .
SMRP08461; 83-173; 204-305; 394-632
IntActP08461; 1; .
STRINGP08461; -; .
PhosphoSiteP08461; -; .
World-2DPAGE0004:P08461; -; .
PRIDEP08461; -; .
EnsemblENSRNOT00000032152; ENSRNOP00000032890; ENSRNOG00000009994; .
GeneID81654; -; .
KEGGrno:81654; -; .
NMPDRfig|10116.3.peg.28575; -; .
UCSCNM_031025; rat; .
CTD1737; -; .
RGD619859; Dlat; .
eggNOGroNOG14419; -; .
GeneTreeENSGT00560000077144; -; .
HOVERGENHBG005063; -; .
InParanoidP08461; -; .
OMAYASPMAK; -; .
OrthoDBEOG412M54; -; .
PhylomeDBP08461; -; .
NextBio615200; -; .
ArrayExpressP08461; -; .
GenevestigatorP08461; -; .
GermOnlineENSRNOG00000009994; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD; .
GOGO:0004742; F:dihydrolipoyllysine-residue acetyltransferase activity; IDA:RGD; .
GOGO:0000166; F:nucleotide binding; TAS:RGD; .
GOGO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
GOGO:0030431; P:sleep; IEP:RGD; .
InterProIPR003016; 2-oxoA_DH_lipoyl-BS; .
InterProIPR001078; 2-oxoacid_DH_actylTfrase; .
InterProIPR006257; AcTrfase_Pyrv_DH_cplx_L; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR023213; CAT-like_dom; .
InterProIPR004167; E3-bd; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.30.559.10; CAT-like_dom; 1; .
Gene3DG3DSA:4.10.320.10; E3_bd; 1; .
KOK00627; -; .
PfamPF00198; 2-oxoacid_dh; 1; .
PfamPF00364; Biotin_lipoyl; 2; .
PfamPF02817; E3_binding; 1; .
SUPFAMSSF47005; E3_bd; 1; .
SUPFAMSSF51230; Hybrid_motif; 2; .
TIGRFAMsTIGR01349; PDHac_trf_mito; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 2; .
PROSITEPS00189; LIPOYL; 2; .



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server