World-2DPAGE Repository
Home
|
Contact
Due to maintenance work, this service and ftp://ftp.expasy.org will be unavailable from Tuesday August 23rd 18:00 until Wednesday August 24th 08:00
CEST
. Apologies for the inconvenience.
!!This displayer contains script commands. You should enable JavaScripting within your browser preferences, or use another browser!
World-2DPAGE Repository
Search by
[accession number]
[description, ID or gene]
[author names]
[spot ID / serial number]
[identification methods]
[pI / Mw range]
[combined fields]
Maps
[experimental info]
[protein list]
[graphical interface]
Databases
0001
0002
0003
0004
0005
0006
0007
0008
0009
0010
0011
0012
0014
0017
0018
0019
0020
0021
0022
0023
0024
0026
0027
0029
0030
0031
0032
0034
0035
0036
0037
0038
0039
0040
0042
0043
0044
0046
0047
0050
0051
0053
0054
0056
0059
0061
0062
0063
0066
0068
0069
0076
0077
0080
0084
0085
0088
use 'Ctrl' to select several
databases
Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
Searching in 'World-2DPAGE Repository [0014]' for entry
matching:
3HIDH_RAT
World-2DPAGE Repository
(0014):
3HIDH_RAT
3HIDH_RAT
General information about the entry
View entry in simple text format
Entry name
3HIDH_RAT
Primary accession number
P29266
integrated into World-2DPAGE Repository (0014) on
October 13, 2008 (release 1)
2D Annotations were last modified on
June 1, 2011 (version 3)
General Annotations were last modified on
November 25, 2011 (version 2)
Name and origin of the protein
Description
RecName: Full=3-hydroxyisobutyrate dehydrogenase, mitochondrial; Short=HIBADH; EC=1.1.1.31; Flags: Precursor;.
Gene name
Name=Hibadh
Annotated species
Rattus norvegicus (Rat) [TaxID:
10116
]
Taxonomy
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]
2D-PAGE GEL CHARACTERIZATION
DOI=
10.1002/pmic.200900680
;
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of whole cerebellum of the mature rat''
Proteomics 10(23):4311-4319 (2010)
2D PAGE maps for identified proteins
How to interpret a protein
RAT_CEREBELLUM_21D
{Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum
map experimental info
RAT_CEREBELLUM_21D
MAP LOCATIONS:
SPOT 3094
:
pI=6.34; Mw=32000
[identification data]
IDENTIFICATION:
SPOT 3094: SeqCov=27%. MascotPMF=45 [
1
].
MAPPING (identification):
SPOT
3094
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
].
Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
PRIDE
10017.
UniProtKB/Swiss-Prot
P29266; 3HIDH_RAT.
2D PAGE maps for identified proteins
How to interpret a protein map
You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
Warning 1
: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
Warning 2
: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
External data extracted from
UniProtKB/Swiss-Prot
Extracted from
UniProtKB/Swiss-Prot
, release:
2011_11
Entry name
3HIDH_RAT
Primary accession number
P29266
Secondary accession number(s)
A1L107
Sequence was last modified on
January 24, 2001 (version 3)
Annotations were last modified on
November 16, 2011 (version 108)
Name and origin of the protein
Description
RecName: Full=3-hydroxyisobutyrate dehydrogenase, mitochondrial; Short=HIBADH; EC=1.1.1.31; Flags: Precursor;
Gene name
Name=Hibadh
Encoded on
Name=Hibadh
Keywords
Complete proteome
;
Direct protein sequencing
;
Mitochondrion
;
NAD
;
Oxidoreductase
;
Reference proteome
;
Transit peptide
.
Copyright
Copyrighted by the UniProt Consortium, see
https://www.uniprot.org/help/license
. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL
J04628; AAA50312.1
; ALT_FRAME; mRNA
EMBL
BC127442; AAI27443.1
; -; mRNA
IPI
IPI00202658; -
; .
PIR
A32867; A32867
; .
RefSeq
NP_071579.1; NM_022243.1
; .
UniGene
Rn.73; -
; .
ProteinModelPortal
P29266; -
; .
SMR
P29266; 40-334
; .
IntAct
P29266; 1
; .
STRING
P29266; -
; .
PRIDE
P29266; -
; .
Ensembl
ENSRNOT00000011069; ENSRNOP00000011069
; ENSRNOG00000008063; .
GeneID
63938; -
; .
KEGG
rno:63938; -
; .
UCSC
NM_022243; rat
; .
CTD
11112; -
; .
RGD
708399; Hibadh
; .
eggNOG
roNOG07166; -
; .
GeneTree
ENSGT00530000063270; -
; .
HOVERGEN
HBG050424; -
; .
InParanoid
P29266; -
; .
OMA
STIDPNV; -
; .
OrthoDB
EOG476K0N; -
; .
PhylomeDB
P29266; -
; .
NextBio
612544; -
; .
ArrayExpress
P29266; -
; .
Genevestigator
P29266; -
; .
GermOnline
ENSRNOG00000008063; Rattus norvegicus
; .
GO
GO:0005739; C:mitochondrion
; IEA:UniProtKB-SubCell; .
GO
GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity
; IEA:EC; .
GO
GO:0051287; F:NAD binding
; IEA:InterPro; .
GO
GO:0004616; F:phosphogluconate dehydrogenase (decarboxylating) activity
; IEA:InterPro; .
GO
GO:0006098; P:pentose-phosphate shunt
; IEA:InterPro; .
GO
GO:0006573; P:valine metabolic process
; IEA:InterPro; .
InterPro
IPR002204; 3-OH-isobutyrate_DH-rel_CS
; .
InterPro
IPR015815; 3hydroxyacid_DH/Rdtase
; .
InterPro
IPR008927; 6-PGluconate_DH_C-like
; .
InterPro
IPR006115; 6PGDH_NADP-bd
; .
InterPro
IPR013328; DH_multihelical
; .
InterPro
IPR011548; IsoBut3OH_DH
; .
InterPro
IPR016040; NAD(P)-bd_dom
; .
Gene3D
G3DSA:3.40.50.720; NAD(P)-bd
; 1; .
Gene3D
G3DSA:1.10.1040.10; Opine_DH
; 1; .
KO
K00020; -
; .
PANTHER
PTHR22981; 3hydroxy_acid_DH
; 1; .
Pfam
PF03446; NAD_binding_2
; 1; .
SUPFAM
SSF48179; 6DGDH_C_like
; 1; .
TIGRFAMs
TIGR01692; HIBADH
; 1; .
PROSITE
PS00895; 3_HYDROXYISOBUT_DH
; 1; .
World-2DPAGE Repository (search AC)
Database constructed and maintained by
SIB
, using the
Make2D-DB II
package (
ver. 3.10.2
) from the
World-2DPAGE Constellation
of the
ExPASy web server