Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2965:

pI=9.46; Mw=42000

View entry in simple text format

IDENTIFICATION: SPOT 2965: SeqCov=43%. MascotPMF=118 [1].
MAPPING (identification):

SPOT 2965: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

P00507; AATM_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	AATM_RAT
Primary accession number	P00507
Secondary accession number(s)	Q64551 Q9QV50
Sequence was last modified on	July 1, 1989 (version 2)
Annotations were last modified on	November 16, 2011 (version 111)
Name and origin of the protein
Description	RecName: Full=Aspartate aminotransferase, mitochondrial; Short=mAspAT; EC=2.6.1.1; AltName: Full=Fatty acid-binding protein; Short=FABP-1; AltName: Full=Glutamate oxaloacetate transaminase 2; AltName: Full=Plasma membrane-associated fatty acid-binding protein; Short=FABPpm; AltName: Full=Transaminase A; Flags: Precursor;
Gene name	Name=Got2 Synonyms=Maat
Encoded on	Name=Got2; Synonyms=Maat
Keywords	Acetylation; Aminotransferase; Cell membrane; Complete proteome; Direct protein sequencing; Lipid transport; Membrane; Mitochondrion; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M18467; AAB54275.1; -; mRNA
EMBL	BC061792; AAH61792.1; -; mRNA
EMBL	U21158; AAC13868.1; -; Genomic_DNA
EMBL	M12709; AAA41267.1; -; mRNA
IPI	IPI00210920; -; .
PIR	A28005; XNRTDM; .
PIR	I55427; I55427; .
RefSeq	NP_037309.1; NM_013177.2; .
UniGene	Rn.98650; -; .
ProteinModelPortal	P00507; -; .
SMR	P00507; 30-430; .
IntAct	P00507; 1; .
STRING	P00507; -; .
PhosphoSite	P00507; -; .
PRIDE	P00507; -; .
Ensembl	ENSRNOT00000015956; ENSRNOP00000015956; ENSRNOG00000011782; .
GeneID	25721; -; .
KEGG	rno:25721; -; .
NMPDR	fig\|10116.3.peg.14535; -; .
UCSC	NM_013177; rat; .
CTD	2806; -; .
RGD	2722; Got2; .
eggNOG	maNOG12574; -; .
GeneTree	ENSGT00390000014081; -; .
HOVERGEN	HBG000951; -; .
InParanoid	P00507; -; .
OMA	AYRDDNT; -; .
OrthoDB	EOG4RXZ07; -; .
PhylomeDB	P00507; -; .
BioCyc	MetaCyc:MONOMER-15069; -; .
BRENDA	2.6.1.1; 5301; .
NextBio	607817; -; .
ArrayExpress	P00507; -; .
Genevestigator	P00507; -; .
GermOnline	ENSRNOG00000011782; Rattus norvegicus; .
GO	GO:0009986; C:cell surface; IDA:RGD; .
GO	GO:0005743; C:mitochondrial inner membrane; IDA:RGD; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0043204; C:perikaryon; IDA:RGD; .
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0043234; C:protein complex; IDA:RGD; .
GO	GO:0016597; F:amino acid binding; IDA:RGD; .
GO	GO:0019899; F:enzyme binding; IDA:RGD; .
GO	GO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; ISS:UniProtKB; .
GO	GO:0080130; F:L-phenylalanine:2-oxoglutarate aminotransferase activity; IEA:EC; .
GO	GO:0005543; F:phospholipid binding; IDA:RGD; .
GO	GO:0042803; F:protein homodimerization activity; IMP:RGD; .
GO	GO:0030170; F:pyridoxal phosphate binding; IDA:RGD; .
GO	GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB; .
GO	GO:0006531; P:aspartate metabolic process; ISS:UniProtKB; .
GO	GO:0009058; P:biosynthetic process; IEA:InterPro; .
GO	GO:0015908; P:fatty acid transport; TAS:RGD; .
GO	GO:0006536; P:glutamate metabolic process; ISS:UniProtKB; .
InterPro	IPR004839; Aminotransferase_I/II; .
InterPro	IPR000796; Asp_trans; .
InterPro	IPR004838; NHTrfase_class1_PyrdxlP-BS; .
InterPro	IPR015424; PyrdxlP-dep_Trfase_major_dom; .
InterPro	IPR015421; PyrdxlP-dep_Trfase_major_sub1; .
Gene3D	G3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 1; .
KO	K14455; -; .
PANTHER	PTHR11879; Asp_trans; 1; .
Pfam	PF00155; Aminotran_1_2; 1; .
PRINTS	PR00799; TRANSAMINASE; .
SUPFAM	SSF53383; PyrdxlP-dep_Trfase_major; 1; .
PROSITE	PS00105; AA_TRANSFER_CLASS_1; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: AATM_RAT

World-2DPAGE Repository (0019): AATM_RAT

AATM_RAT

General information about the entry

Entry name

AATM_RAT

Primary accession number

P00507

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

Gene name

Name=Got2
Synonyms=Maat

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 1112:

pI=9.13; Mw=47683

SPOT 1200:

pI=9.13; Mw=47683

View entry in simple text format

IDENTIFICATION: SPOT 1112: Peptides number=11. SeqCov=21.9%. MOWSE score=1.86E+5 [1]
SPOT 1200: Peptides number=8. SeqCov=27.7%. MOWSE score=8.00E+4 [1].
MAPPING (identification):

SPOT 1112: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1200: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

P00507; AATM_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	AATM_RAT
Primary accession number	P00507
Secondary accession number(s)	Q64551 Q9QV50
Sequence was last modified on	July 1, 1989 (version 2)
Annotations were last modified on	November 16, 2011 (version 111)
Name and origin of the protein
Description	RecName: Full=Aspartate aminotransferase, mitochondrial; Short=mAspAT; EC=2.6.1.1; AltName: Full=Fatty acid-binding protein; Short=FABP-1; AltName: Full=Glutamate oxaloacetate transaminase 2; AltName: Full=Plasma membrane-associated fatty acid-binding protein; Short=FABPpm; AltName: Full=Transaminase A; Flags: Precursor;
Gene name	Name=Got2 Synonyms=Maat
Encoded on	Name=Got2; Synonyms=Maat
Keywords	Acetylation; Aminotransferase; Cell membrane; Complete proteome; Direct protein sequencing; Lipid transport; Membrane; Mitochondrion; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M18467; AAB54275.1; -; mRNA
EMBL	BC061792; AAH61792.1; -; mRNA
EMBL	U21158; AAC13868.1; -; Genomic_DNA
EMBL	M12709; AAA41267.1; -; mRNA
IPI	IPI00210920; -; .
PIR	A28005; XNRTDM; .
PIR	I55427; I55427; .
RefSeq	NP_037309.1; NM_013177.2; .
UniGene	Rn.98650; -; .
ProteinModelPortal	P00507; -; .
SMR	P00507; 30-430; .
IntAct	P00507; 1; .
STRING	P00507; -; .
PhosphoSite	P00507; -; .
PRIDE	P00507; -; .
Ensembl	ENSRNOT00000015956; ENSRNOP00000015956; ENSRNOG00000011782; .
GeneID	25721; -; .
KEGG	rno:25721; -; .
NMPDR	fig\|10116.3.peg.14535; -; .
UCSC	NM_013177; rat; .
CTD	2806; -; .
RGD	2722; Got2; .
eggNOG	maNOG12574; -; .
GeneTree	ENSGT00390000014081; -; .
HOVERGEN	HBG000951; -; .
InParanoid	P00507; -; .
OMA	AYRDDNT; -; .
OrthoDB	EOG4RXZ07; -; .
PhylomeDB	P00507; -; .
BioCyc	MetaCyc:MONOMER-15069; -; .
BRENDA	2.6.1.1; 5301; .
NextBio	607817; -; .
ArrayExpress	P00507; -; .
Genevestigator	P00507; -; .
GermOnline	ENSRNOG00000011782; Rattus norvegicus; .
GO	GO:0009986; C:cell surface; IDA:RGD; .
GO	GO:0005743; C:mitochondrial inner membrane; IDA:RGD; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0043204; C:perikaryon; IDA:RGD; .
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0043234; C:protein complex; IDA:RGD; .
GO	GO:0016597; F:amino acid binding; IDA:RGD; .
GO	GO:0019899; F:enzyme binding; IDA:RGD; .
GO	GO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; ISS:UniProtKB; .
GO	GO:0080130; F:L-phenylalanine:2-oxoglutarate aminotransferase activity; IEA:EC; .
GO	GO:0005543; F:phospholipid binding; IDA:RGD; .
GO	GO:0042803; F:protein homodimerization activity; IMP:RGD; .
GO	GO:0030170; F:pyridoxal phosphate binding; IDA:RGD; .
GO	GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB; .
GO	GO:0006531; P:aspartate metabolic process; ISS:UniProtKB; .
GO	GO:0009058; P:biosynthetic process; IEA:InterPro; .
GO	GO:0015908; P:fatty acid transport; TAS:RGD; .
GO	GO:0006536; P:glutamate metabolic process; ISS:UniProtKB; .
InterPro	IPR004839; Aminotransferase_I/II; .
InterPro	IPR000796; Asp_trans; .
InterPro	IPR004838; NHTrfase_class1_PyrdxlP-BS; .
InterPro	IPR015424; PyrdxlP-dep_Trfase_major_dom; .
InterPro	IPR015421; PyrdxlP-dep_Trfase_major_sub1; .
Gene3D	G3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 1; .
KO	K14455; -; .
PANTHER	PTHR11879; Asp_trans; 1; .
Pfam	PF00155; Aminotran_1_2; 1; .
PRINTS	PR00799; TRANSAMINASE; .
SUPFAM	SSF53383; PyrdxlP-dep_Trfase_major; 1; .
PROSITE	PS00105; AA_TRANSFER_CLASS_1; 1; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: AATM_RAT

World-2DPAGE Repository (0022): AATM_RAT

AATM_RAT

General information about the entry

Entry name

AATM_RAT

Primary accession number

P00507

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

Gene name

Name=Got2
Synonyms=Maat

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 175:

pI=9.13; Mw=47683

SPOT 669:

pI=9.13; Mw=47683

SPOT 770:

pI=9.13; Mw=47683

View entry in simple text format

IDENTIFICATION: SPOT 175: SeqCov=23.7%. Peptides MS=10/20. Mascot PMF Score=66 [1]
SPOT 669: SeqCov=19.8%. Peptides MS=8/20. Mascot PMF Score=51 [1]; SPOT 770: SeqCov=21.6%. Peptides MS=9/20. Mascot PMF Score=61 [1].
PEPTIDE EVIDENCE: SPOT 669: 126-FVTVQTISGTGALR-139 (60) [1]
SPOT 770: 140-VGASFLQR-147 (27) / 126-FVTVQTISGTGALR-139 (79) [1].
MAPPING (identification):

SPOT 175: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 669: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 770: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot

P00507; AATM_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	AATM_RAT
Primary accession number	P00507
Secondary accession number(s)	Q64551 Q9QV50
Sequence was last modified on	July 1, 1989 (version 2)
Annotations were last modified on	November 16, 2011 (version 111)
Name and origin of the protein
Description	RecName: Full=Aspartate aminotransferase, mitochondrial; Short=mAspAT; EC=2.6.1.1; AltName: Full=Fatty acid-binding protein; Short=FABP-1; AltName: Full=Glutamate oxaloacetate transaminase 2; AltName: Full=Plasma membrane-associated fatty acid-binding protein; Short=FABPpm; AltName: Full=Transaminase A; Flags: Precursor;
Gene name	Name=Got2 Synonyms=Maat
Encoded on	Name=Got2; Synonyms=Maat
Keywords	Acetylation; Aminotransferase; Cell membrane; Complete proteome; Direct protein sequencing; Lipid transport; Membrane; Mitochondrion; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M18467; AAB54275.1; -; mRNA
EMBL	BC061792; AAH61792.1; -; mRNA
EMBL	U21158; AAC13868.1; -; Genomic_DNA
EMBL	M12709; AAA41267.1; -; mRNA
IPI	IPI00210920; -; .
PIR	A28005; XNRTDM; .
PIR	I55427; I55427; .
RefSeq	NP_037309.1; NM_013177.2; .
UniGene	Rn.98650; -; .
ProteinModelPortal	P00507; -; .
SMR	P00507; 30-430; .
IntAct	P00507; 1; .
STRING	P00507; -; .
PhosphoSite	P00507; -; .
PRIDE	P00507; -; .
Ensembl	ENSRNOT00000015956; ENSRNOP00000015956; ENSRNOG00000011782; .
GeneID	25721; -; .
KEGG	rno:25721; -; .
NMPDR	fig\|10116.3.peg.14535; -; .
UCSC	NM_013177; rat; .
CTD	2806; -; .
RGD	2722; Got2; .
eggNOG	maNOG12574; -; .
GeneTree	ENSGT00390000014081; -; .
HOVERGEN	HBG000951; -; .
InParanoid	P00507; -; .
OMA	AYRDDNT; -; .
OrthoDB	EOG4RXZ07; -; .
PhylomeDB	P00507; -; .
BioCyc	MetaCyc:MONOMER-15069; -; .
BRENDA	2.6.1.1; 5301; .
NextBio	607817; -; .
ArrayExpress	P00507; -; .
Genevestigator	P00507; -; .
GermOnline	ENSRNOG00000011782; Rattus norvegicus; .
GO	GO:0009986; C:cell surface; IDA:RGD; .
GO	GO:0005743; C:mitochondrial inner membrane; IDA:RGD; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0043204; C:perikaryon; IDA:RGD; .
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0043234; C:protein complex; IDA:RGD; .
GO	GO:0016597; F:amino acid binding; IDA:RGD; .
GO	GO:0019899; F:enzyme binding; IDA:RGD; .
GO	GO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; ISS:UniProtKB; .
GO	GO:0080130; F:L-phenylalanine:2-oxoglutarate aminotransferase activity; IEA:EC; .
GO	GO:0005543; F:phospholipid binding; IDA:RGD; .
GO	GO:0042803; F:protein homodimerization activity; IMP:RGD; .
GO	GO:0030170; F:pyridoxal phosphate binding; IDA:RGD; .
GO	GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB; .
GO	GO:0006531; P:aspartate metabolic process; ISS:UniProtKB; .
GO	GO:0009058; P:biosynthetic process; IEA:InterPro; .
GO	GO:0015908; P:fatty acid transport; TAS:RGD; .
GO	GO:0006536; P:glutamate metabolic process; ISS:UniProtKB; .
InterPro	IPR004839; Aminotransferase_I/II; .
InterPro	IPR000796; Asp_trans; .
InterPro	IPR004838; NHTrfase_class1_PyrdxlP-BS; .
InterPro	IPR015424; PyrdxlP-dep_Trfase_major_dom; .
InterPro	IPR015421; PyrdxlP-dep_Trfase_major_sub1; .
Gene3D	G3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 1; .
KO	K14455; -; .
PANTHER	PTHR11879; Asp_trans; 1; .
Pfam	PF00155; Aminotran_1_2; 1; .
PRINTS	PR00799; TRANSAMINASE; .
SUPFAM	SSF53383; PyrdxlP-dep_Trfase_major; 1; .
PROSITE	PS00105; AA_TRANSFER_CLASS_1; 1; .

Searching in 'World-2DPAGE Repository [0069]' for entry matching: AATM_RAT

World-2DPAGE Repository (0069): AATM_RAT

AATM_RAT

General information about the entry

Entry name

AATM_RAT

Primary accession number

AATM_RAT

integrated into World-2DPAGE Repository (0069) on

February 27, 2014 (release 1)

2D Annotations were last modified on

February 27, 2014 (version 1)

General Annotations were last modified on

February 27, 2014 (version 1)

Name and origin of the protein

Description

AATM_RAT.

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D GEL CHARACTERIZATION
DOI=10.1016/j.jprot.2014.04.015;
Burniston J.G., Kenyani J., Gray D., Guadagnin E., Jarman I.H., Cobley J.N., Cuthbertson D.J., Chen Y.-W., Wastling J.M., Lisboa P., Koch L.G., Britton S.L.
''Conditional independence mapping of DIGE data reveals PDIA3 protein species as key nodes associated with muscle aerobic capacity''
Journal of Proteomics 106(1):230-245 (2014)

2D PAGE maps for identified proteins

RATTUS_SOLEUS-DIGE_4.5-9.5 {M+F HCR LCR Soleus DiGE Exp}
Rattus
Tissue: Skeletal muscle