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Searching in 'World-2DPAGE Repository [0022]' for entry matching: ACADL_RAT




World-2DPAGE Repository (0022):  ACADL_RAT


ACADL_RAT


General information about the entry
View entry in simple text format
Entry nameACADL_RAT
Primary accession numberP15650
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Long-chain specific acyl-CoA dehydrogenase, mitochondrial; Short=LCAD; EC=1.3.99.13; Flags: Precursor;.
Gene nameName=Acadl
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=7.63; Mw=48242  [identification data]
pI=7.63; Mw=48242  [identification data]
pI=7.63; Mw=48242  [identification data]
pI=7.63; Mw=48242  [identification data]
pI=7.63; Mw=48242  [identification data]
pI=7.63; Mw=48242  [identification data]
pI=7.63; Mw=48242  [identification data]

IDENTIFICATION: SPOT 298: SeqCov=21.4%. Peptides MS=12/20. Mascot PMF Score=93 [1]
SPOT 362: SeqCov=29.5%. Peptides MS=15/20. Mascot PMF Score=137 [1]; SPOT 474: SeqCov=27.7%. Peptides MS=13/20. Mascot PMF Score=105 [1]; SPOT 564: SeqCov=23%. Peptides MS=12/20. Mascot PMF Score=89 [1]; SPOT 627: SeqCov=13.7%. Peptides MS=6/20. Mascot PMF Score=34 [1]; SPOT 651: SeqCov=20%. Peptides MS=8/20. Mascot PMF Score=57 [1]; SPOT 901: SeqCov=33.3%. Peptides MS=15/20. Mascot PMF Score=138 [1].
PEPTIDE EVIDENCE: SPOT 298: 52-IFSSEHDIFR-61 (61) / 255-AQDTAELFFEDVR-267 (77) / 280-GFYYLMQELPQER-292 (53) [1]
SPOT 474: 255-AQDTAELFFEDVR-267 (85) / 280-GFYYLMQELPQER-292 (52) / 293-LLIADLAISACEFMFEETR-311 (138) [1]; SPOT 564: 255-AQDTAELFFEDVR-267 (94) / 280-GFYYLMQELPQER-292 (45) / 293-LLIADLAISACEFMFEETR-311 (100) [1]; SPOT 627: 52-IFSSEHDIFR-61 (37) / 255-AQDTAELFFEDVR-267 (64) [1].
MAPPING (identification):
SPOT 298: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 362: Peptide mass fingerprinting [1];
SPOT 474: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 564: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 627: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 651: Peptide mass fingerprinting [1];
SPOT 901: Peptide mass fingerprinting [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP15650; ACADL_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameACADL_RAT
Primary accession numberP15650
Sequence was last modified on April 1, 1990 (version 1)
Annotations were last modified on November 16, 2011 (version 107)
Name and origin of the protein
DescriptionRecName: Full=Long-chain specific acyl-CoA dehydrogenase, mitochondrial; Short=LCAD; EC=1.3.99.13; Flags: Precursor;
Gene nameName=Acadl
Encoded onName=Acadl
KeywordsComplete proteome; Direct protein sequencing; FAD; Fatty acid metabolism; Flavoprotein; Lipid metabolism; Mitochondrion; Oxidoreductase; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ05029; AAA40668.1; -; mRNA
EMBLL11276; AAA41514.1; -; mRNA
EMBLBC062006; AAH62006.1; -; mRNA
IPIIPI00211225; -; .
PIRA34252; A34252; .
RefSeqNP_036951.1; NM_012819.1; .
UniGeneRn.174; -; .
ProteinModelPortalP15650; -; .
IntActP15650; 1; .
STRINGP15650; -; .
PRIDEP15650; -; .
EnsemblENSRNOT00000017686; ENSRNOP00000017686; ENSRNOG00000012966; .
GeneID25287; -; .
KEGGrno:25287; -; .
UCSCNM_012819; rat; .
CTD33; -; .
RGD2011; Acadl; .
eggNOGroNOG08008; -; .
GeneTreeENSGT00590000082906; -; .
HOVERGENHBG104903; -; .
InParanoidP15650; -; .
OMAVSRELWE; -; .
OrthoDBEOG4FR0RR; -; .
PhylomeDBP15650; -; .
NextBio606021; -; .
ArrayExpressP15650; -; .
GenevestigatorP15650; -; .
GermOnlineENSRNOG00000012966; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IDA:BHF-UCL; .
GOGO:0031966; C:mitochondrial membrane; IDA:BHF-UCL; .
GOGO:0000062; F:fatty-acyl-CoA binding; IDA:RGD; .
GOGO:0050660; F:flavin adenine dinucleotide binding; IDA:RGD; .
GOGO:0004466; F:long-chain-acyl-CoA dehydrogenase activity; IDA:RGD; .
GOGO:0033539; P:fatty acid beta-oxidation using acyl-CoA dehydrogenase; IDA:RGD; .
GOGO:0042758; P:long-chain fatty acid catabolic process; IDA:RGD; .
GOGO:0051289; P:protein homotetramerization; IDA:RGD; .
InterProIPR006089; Acyl-CoA_DH_CS; .
InterProIPR006092; Acyl-CoA_DH_N; .
InterProIPR006090; Acyl-CoA_Oxase/DH_1; .
InterProIPR006091; Acyl-CoA_Oxase/DH_cen-dom; .
InterProIPR009075; AcylCo_DH/oxidase_C; .
InterProIPR013786; AcylCoA_DH/ox_N; .
InterProIPR009100; AcylCoA_DH/oxidase; .
Gene3DG3DSA:2.40.110.10; Acyl_CoA_DH/ox_M; 1; .
Gene3DG3DSA:1.10.540.10; AcylCoA_DH/ox_N; 1; .
Gene3DG3DSA:1.20.140.10; AcylCoA_DH_1/2_C; 1; .
KOK00255; -; .
PfamPF00441; Acyl-CoA_dh_1; 1; .
PfamPF02770; Acyl-CoA_dh_M; 1; .
PfamPF02771; Acyl-CoA_dh_N; 1; .
SUPFAMSSF56645; AcylCoA_dehyd_NM; 1; .
SUPFAMSSF47203; AcylCoADH_C_like; 1; .
PROSITEPS00072; ACYL_COA_DH_1; 1; .
PROSITEPS00073; ACYL_COA_DH_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server