World-2DPAGE Repository

RecName: Full=Cytoplasmic aconitate hydratase; Short=Aconitase; EC=4.2.1.3; AltName: Full=Citrate hydro-lyase; AltName: Full=Iron regulatory protein 1; Short=IRP1; AltName: Full=Iron-responsive element-binding protein 1; Short=IRE-BP 1;.

Gene name

Name=Aco1
Synonyms=Ireb1, Irebp

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

map experimental info

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2706:

pI=7.73; Mw=76000

[identification data]

IDENTIFICATION: SPOT 2706: SeqCov=26%. MascotPMF=112 [1].
MAPPING (identification):

SPOT 2706: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

Q63270; ACOC_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	ACOC_RAT
Primary accession number	Q63270
Sequence was last modified on	November 1, 1996 (version 1)
Annotations were last modified on	November 16, 2011 (version 97)
Name and origin of the protein
Description	RecName: Full=Cytoplasmic aconitate hydratase; Short=Aconitase; EC=4.2.1.3; AltName: Full=Citrate hydro-lyase; AltName: Full=Iron regulatory protein 1; Short=IRP1; AltName: Full=Iron-responsive element-binding protein 1; Short=IRE-BP 1;
Gene name	Name=Aco1 Synonyms=Ireb1, Irebp
Encoded on	Name=Aco1; Synonyms=Ireb1, Irebp
Keywords	4Fe-4S; Complete proteome; Cytoplasm; Direct protein sequencing; Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome; RNA-binding; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	L23874; AAA41449.1; -; mRNA
IPI	IPI00207003; -; .
PIR	A44154; A44154; .
RefSeq	NP_059017.1; NM_017321.1; .
UniGene	Rn.35934; -; .
ProteinModelPortal	Q63270; -; .
SMR	Q63270; 3-889; .
STRING	Q63270; -; .
PhosphoSite	Q63270; -; .
PRIDE	Q63270; -; .
GeneID	50655; -; .
KEGG	rno:50655; -; .
UCSC	NM_017321; rat; .
CTD	48; -; .
RGD	2019; Aco1; .
eggNOG	roNOG06028; -; .
GeneTree	ENSGT00530000063060; -; .
HOVERGEN	HBG052147; -; .
OrthoDB	EOG4C2H8W; -; .
PhylomeDB	Q63270; -; .
NextBio	610466; -; .
ArrayExpress	Q63270; -; .
Genevestigator	Q63270; -; .
GermOnline	ENSRNOG00000005849; Rattus norvegicus; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0005783; C:endoplasmic reticulum; IDA:RGD; .
GO	GO:0005794; C:Golgi apparatus; IDA:MGI; .
GO	GO:0005792; C:microsome; IDA:RGD; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0051539; F:4 iron; 4 sulfur cluster binding; ISS:UniProtKB
GO	GO:0003994; F:aconitate hydratase activity; ISS:UniProtKB; .
GO	GO:0052632; F:citrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC; .
GO	GO:0030350; F:iron-responsive element binding; ISS:UniProtKB; .
GO	GO:0052633; F:isocitrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC; .
GO	GO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GO	GO:0048027; F:mRNA 5'-UTR binding; IDA:RGD; .
GO	GO:0006879; P:cellular iron ion homeostasis; IDA:RGD; .
GO	GO:0006101; P:citrate metabolic process; ISS:UniProtKB; .
GO	GO:0006417; P:regulation of translation; TAS:RGD; .
GO	GO:0010040; P:response to iron(II) ion; ISS:UniProtKB; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3; .
InterPro	IPR015937; Acoase/IPM_deHydtase; .
InterPro	IPR001030; Acoase/IPM_deHydtase_lsu_aba; .
InterPro	IPR015928; Aconitase/3IPM_dehydase_swvl; .
InterPro	IPR006249; Aconitase/Fe_reg_prot_2; .
InterPro	IPR015934; Aconitase/Fe_reg_prot_2/AcnD; .
InterPro	IPR015932; Aconitase/IPMdHydase_lsu_aba_2; .
InterPro	IPR018136; Aconitase_4Fe-4S_BS; .
InterPro	IPR000573; AconitaseA/IPMdHydase_ssu_swvl; .
Gene3D	G3DSA:3.30.499.10; Acnase/IPM_dHydase_lsu_aba_1/3; 3; .
Gene3D	G3DSA:3.20.19.10; Aconitase/3IPM_dehydase_swvl; 1; .
Gene3D	G3DSA:3.40.1060.10; Aconitase/IPMdHydase_lsu_aba_2; 1; .
KO	K01681; -; .
PANTHER	PTHR11670; Aconitase-like_core; 1; .
PANTHER	PTHR11670:SF1; Aconitase/Fe_reg_prot_2/AcnD; 1; .
Pfam	PF00330; Aconitase; 1; .
Pfam	PF00694; Aconitase_C; 1; .
PRINTS	PR00415; ACONITASE; .
SUPFAM	SSF52016; Aconitase/3IPM_dehydase_swvl; 1; .
SUPFAM	SSF53732; Aconitase_N; 1; .
TIGRFAMs	TIGR01341; Aconitase_1; 1; .
PROSITE	PS00450; ACONITASE_1; 1; .
PROSITE	PS01244; ACONITASE_2; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server

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