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Searching in 'World-2DPAGE Repository [0022]' for entry matching: ACSL1_RAT




World-2DPAGE Repository (0022):  ACSL1_RAT

ACSL1_RAT


General information about the entry
View entry in simple text format
Entry nameACSL1_RAT
Primary accession numberP18163
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Long-chain-fatty-acid--CoA ligase 1; EC=6.2.1.3; AltName: Full=Long-chain acyl-CoA synthetase 1; Short=LACS 1; AltName: Full=Long-chain-fatty-acid--CoA ligase, liver isozyme;.
Gene nameName=Acsl1
Synonyms=Acs1, Acsl2, Facl2
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.60; Mw=79155  [identification data]

IDENTIFICATION: SPOT 535: SeqCov=20.9%. Peptides MS=11/20. Mascot PMF Score=86 [1].
PEPTIDE EVIDENCE: SPOT 535: 342-IGFFQGDIR-350 (35) / 358-VLQPTIFPVVPR-369 (46) / 74-SAVLEDDKLLLYYYDDVR-91 (92) [1].
MAPPING (identification):
SPOT 535: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP18163; ACSL1_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameACSL1_RAT
Primary accession numberP18163
Sequence was last modified on November 1, 1990 (version 1)
Annotations were last modified on November 16, 2011 (version 104)
Name and origin of the protein
DescriptionRecName: Full=Long-chain-fatty-acid--CoA ligase 1; EC=6.2.1.3; AltName: Full=Long-chain acyl-CoA synthetase 1; Short=LACS 1; AltName: Full=Long-chain-fatty-acid--CoA ligase, liver isozyme;
Gene nameName=Acsl1
Synonyms=Acs1, Acsl2, Facl2
Encoded onName=Acsl1; Synonyms=Acs1, Acsl2, Facl2
KeywordsAcetylation; ATP-binding; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Fatty acid metabolism; Ligase; Lipid metabolism; Magnesium; Membrane; Microsome; Mitochondrion; Mitochondrion outer membrane; Nitration; Nucleotide-binding; Peroxisome; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD90109; BAA14136.1; -; mRNA
IPIIPI00188989; -; .
PIRA36275; A36275; .
RefSeqNP_036952.1; NM_012820.1; .
UniGeneRn.6215; -; .
ProteinModelPortalP18163; -; .
IntActP18163; 28; .
STRINGP18163; -; .
PhosphoSiteP18163; -; .
PRIDEP18163; -; .
EnsemblENSRNOT00000014235; ENSRNOP00000014235; ENSRNOG00000010633; .
GeneID25288; -; .
KEGGrno:25288; -; .
NMPDRfig|10116.3.peg.12441; -; .
UCSCNM_012820; rat; .
CTD2180; -; .
RGD2015; Acsl1; .
eggNOGroNOG13164; -; .
GeneTreeENSGT00600000084142; -; .
HOVERGENHBG050452; -; .
InParanoidP18163; -; .
OMASECIGSA; -; .
OrthoDBEOG41JZBN; -; .
PhylomeDBP18163; -; .
BioCycMetaCyc:MONOMER-14442; -; .
BRENDA6.2.1.3; 5301; .
NextBio606025; -; .
ArrayExpressP18163; -; .
GenevestigatorP18163; -; .
GermOnlineENSRNOG00000010633; Rattus norvegicus; .
GOGO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell; .
GOGO:0016021; C:integral to membrane; IEA:UniProtKB-KW; .
GOGO:0005792; C:microsome; IEA:UniProtKB-SubCell; .
GOGO:0005741; C:mitochondrial outer membrane; IDA:RGD; .
GOGO:0005778; C:peroxisomal membrane; IDA:HGNC; .
GOGO:0005886; C:plasma membrane; IDA:RGD; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0003987; F:acetate-CoA ligase activity; TAS:RGD; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004467; F:long-chain fatty acid-CoA ligase activity; IDA:RGD; .
GOGO:0006631; P:fatty acid metabolic process; IMP:RGD; .
GOGO:0042493; P:response to drug; IEP:RGD; .
GOGO:0007584; P:response to nutrient; IEP:RGD; .
GOGO:0034201; P:response to oleic acid; IEP:RGD; .
GOGO:0014070; P:response to organic cyclic compound; IEP:RGD; .
GOGO:0042178; P:xenobiotic catabolic process; IDA:RGD; .
InterProIPR020845; AMP-binding_CS; .
InterProIPR000873; AMP-dep_Synth/Lig; .
KOK01897; -; .
PfamPF00501; AMP-binding; 1; .
PROSITEPS00455; AMP_BINDING; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server