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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0022]' for entry matching: ACSL1_RAT World-2DPAGE Repository (0022):  ACSL1_RAT ACSL1_RAT General information about the entry View entry in simple text format Entry name ACSL1_RAT Primary accession number P18163 integrated into World-2DPAGE Repository (0022) on  May 7, 2010 (release 1) 2D Annotations were last modified on June 22, 2011 (version 2) General Annotations were last modified on  November 24, 2011 (version 2) Name and origin of the protein Description RecName: Full=Long-chain-fatty-acid--CoA ligase 1; EC=6.2.1.3; AltName: Full=Long-chain acyl-CoA synthetase 1; Short=LACS 1; AltName: Full=Long-chain-fatty-acid--CoA ligase, liver isozyme;. Gene name Name=Acsl1 Synonyms=Acs1, Acsl2, Facl2 Annotated species Rattus [TaxID: 10114] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000593; Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L. ''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity'' Proteomics 11(16):3369-3379 (2011) 2D PAGE maps for identified proteins How to interpret a protein HCR_LCR_HEART {HCR LCR Cardiac Map} Rattus Tissue: Heart   map experimental info   HCR_LCR_HEART MAP LOCATIONS: SPOT 535: pI=6.60; Mw=79155   [identification data] IDENTIFICATION: SPOT 535: SeqCov=20.9%. Peptides MS=11/20. Mascot PMF Score=86 [1]. PEPTIDE EVIDENCE: SPOT 535: 342-IGFFQGDIR-350 (35) / 358-VLQPTIFPVVPR-369 (46) / 74-SAVLEDDKLLLYYYDDVR-91 (92) [1]. MAPPING (identification): SPOT 535: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]. Copyright Data from Dr. Jatin Burniston, Liverpool John Moores University, UK Cross-references UniProtKB/Swiss-Prot P18163; ACSL1_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name ACSL1_RAT Primary accession number P18163 Sequence was last modified on  November 1, 1990 (version 1) Annotations were last modified on  November 16, 2011 (version 104) Name and origin of the protein Description RecName: Full=Long-chain-fatty-acid--CoA ligase 1; EC=6.2.1.3; AltName: Full=Long-chain acyl-CoA synthetase 1; Short=LACS 1; AltName: Full=Long-chain-fatty-acid--CoA ligase, liver isozyme; Gene name Name=Acsl1 Synonyms=Acs1, Acsl2, Facl2 Encoded on Name=Acsl1; Synonyms=Acs1, Acsl2, Facl2 Keywords Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Fatty acid metabolism; Ligase; Lipid metabolism; Magnesium; Membrane; Microsome; Mitochondrion; Mitochondrion outer membrane; Nitration; Nucleotide-binding; Peroxisome; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL D90109; BAA14136.1; -; mRNA IPI IPI00188989; -; . PIR A36275; A36275; . RefSeq NP_036952.1; NM_012820.1; . UniGene Rn.6215; -; . ProteinModelPortal P18163; -; . IntAct P18163; 28; . STRING P18163; -; . PhosphoSite P18163; -; . PRIDE P18163; -; . Ensembl ENSRNOT00000014235; ENSRNOP00000014235; ENSRNOG00000010633; . GeneID 25288; -; . KEGG rno:25288; -; . NMPDR fig|10116.3.peg.12441; -; . UCSC NM_012820; rat; . CTD 2180; -; . RGD 2015; Acsl1; . eggNOG roNOG13164; -; . GeneTree ENSGT00600000084142; -; . HOVERGEN HBG050452; -; . InParanoid P18163; -; . OMA SECIGSA; -; . OrthoDB EOG41JZBN; -; . PhylomeDB P18163; -; . BioCyc MetaCyc:MONOMER-14442; -; . BRENDA 6.2.1.3; 5301; . NextBio 606025; -; . ArrayExpress P18163; -; . Genevestigator P18163; -; . GermOnline ENSRNOG00000010633; Rattus norvegicus; . GO GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell; . GO GO:0016021; C:integral to membrane; IEA:UniProtKB-KW; . GO GO:0005792; C:microsome; IEA:UniProtKB-SubCell; . GO GO:0005741; C:mitochondrial outer membrane; IDA:RGD; . GO GO:0005778; C:peroxisomal membrane; IDA:HGNC; . GO GO:0005886; C:plasma membrane; IDA:RGD; . GO GO:0005625; C:soluble fraction; IDA:RGD; . GO GO:0003987; F:acetate-CoA ligase activity; TAS:RGD; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0004467; F:long-chain fatty acid-CoA ligase activity; IDA:RGD; . GO GO:0006631; P:fatty acid metabolic process; IMP:RGD; . GO GO:0042493; P:response to drug; IEP:RGD; . GO GO:0007584; P:response to nutrient; IEP:RGD; . GO GO:0034201; P:response to oleic acid; IEP:RGD; . GO GO:0014070; P:response to organic cyclic compound; IEP:RGD; . GO GO:0042178; P:xenobiotic catabolic process; IDA:RGD; . InterPro IPR020845; AMP-binding_CS; . InterPro IPR000873; AMP-dep_Synth/Lig; . KO K01897; -; . Pfam PF00501; AMP-binding; 1; . PROSITE PS00455; AMP_BINDING; 1; . World-2DPAGE Repository (search AC)