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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: ACTB_RAT World-2DPAGE Repository (0030):  ACTB_RAT ACTB_RAT General information about the entry View entry in simple text format Entry name ACTB_RAT Primary accession number P60711 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed;. Gene name Name=Actb Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 1303: pI=5.07; Mw=52372   [identification data] SPOT 1306: pI=5.10; Mw=52227   [identification data] SPOT 1307: pI=5.14; Mw=52227   [identification data] SPOT 1309: pI=5.32; Mw=52083   [identification data] SPOT 1310: pI=5.35; Mw=52155   [identification data] SPOT 1316: pI=5.25; Mw=51795   [identification data] SPOT 1323: pI=5.21; Mw=51509   [identification data] IDENTIFICATION: SPOT 1303: Mascot protein score=147. Number of peptides=3 [1] SPOT 1306: Mascot protein score=81. Number of peptides=4 [1]; SPOT 1307: Mascot protein score=122. Number of peptides=6 [1]; SPOT 1309: Mascot protein score=85. Number of peptides=3 [1]; SPOT 1310: Mascot protein score=35. Number of peptides=4 [1]; SPOT 1316: Mascot protein score=560. Number of peptides=6 [1]; SPOT 1323: Mascot protein score=50. Number of peptides=3 [1]. MAPPING (identification): SPOT 1303: Tandem mass spectrometry [1]; SPOT 1306: Tandem mass spectrometry [1]; SPOT 1307: Tandem mass spectrometry [1]; SPOT 1309: Tandem mass spectrometry [1]; SPOT 1310: Tandem mass spectrometry [1]; SPOT 1316: Tandem mass spectrometry [1]; SPOT 1323: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot P60711; ACTB_RAT. World-2DPAGE Repository P60711; ACTB_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name ACTB_RAT Primary accession number P60711 Secondary accession number(s) P02570 P70514 P99021 Q11211 Q64316 Sequence was last modified on  April 1, 1988 (version 1) Annotations were last modified on  November 16, 2011 (version 79) Name and origin of the protein Description RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed; Gene name Name=Actb Encoded on Name=Actb Keywords Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Methylation; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL V01217; CAA24528.1; -; Genomic_DNA EMBL BC063166; AAH63166.1; -; mRNA IPI IPI00189819; -; . RefSeq NP_112406.1; NM_031144.2; . UniGene Rn.94978; -; . ProteinModelPortal P60711; -; . SMR P60711; 2-375; . IntAct P60711; 8; . MINT MINT-132123; -; . STRING P60711; -; . PhosphoSite P60711; -; . World-2DPAGE 0004:P60711; -; . PRIDE P60711; -; . Ensembl ENSRNOT00000042459; ENSRNOP00000044296; ENSRNOG00000034254; . GeneID 81822; -; . KEGG rno:81822; -; . UCSC NM_031144; rat; . CTD 60; -; . RGD 628837; Actb; . eggNOG maNOG08069; -; . HOVERGEN HBG003771; -; . InParanoid P60711; -; . OMA IKNLMER; -; . OrthoDB EOG41JZC9; -; . PhylomeDB P60711; -; . Reactome REACT_112697; Developmental Biology; . NextBio 615747; -; . ArrayExpress P60711; -; . Genevestigator P60711; -; . GermOnline ENSRNOG00000034254; Rattus norvegicus; . GO GO:0030424; C:axon; IDA:RGD; . GO GO:0005829; C:cytosol; TAS:Reactome; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0019901; F:protein kinase binding; IPI:RGD; . GO GO:0007409; P:axonogenesis; IDA:RGD; . InterPro IPR004000; Actin-like; . InterPro IPR020902; Actin/actin-like_CS; . InterPro IPR004001; Actin_CS; . KO K05692; -; . PANTHER PTHR11937; Actin_like; 1; . Pfam PF00022; Actin; 1; . PRINTS PR00190; ACTIN; . SMART SM00268; ACTIN; 1; . PROSITE PS00406; ACTINS_1; 1; . PROSITE PS00432; ACTINS_2; 1; . PROSITE PS01132; ACTINS_ACT_LIKE; 1; . World-2DPAGE Repository (search AC)