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Searching in 'World-2DPAGE Repository [0030]' for entry matching: ACTB_RAT




World-2DPAGE Repository (0030):  ACTB_RAT

ACTB_RAT


General information about the entry
View entry in simple text format
Entry nameACTB_RAT
Primary accession numberP60711
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed;.
Gene nameName=Actb
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.07; Mw=52372  [identification data]
pI=5.10; Mw=52227  [identification data]
pI=5.14; Mw=52227  [identification data]
pI=5.32; Mw=52083  [identification data]
pI=5.35; Mw=52155  [identification data]
pI=5.25; Mw=51795  [identification data]
pI=5.21; Mw=51509  [identification data]

IDENTIFICATION: SPOT 1303: Mascot protein score=147. Number of peptides=3 [1]
SPOT 1306: Mascot protein score=81. Number of peptides=4 [1]; SPOT 1307: Mascot protein score=122. Number of peptides=6 [1]; SPOT 1309: Mascot protein score=85. Number of peptides=3 [1]; SPOT 1310: Mascot protein score=35. Number of peptides=4 [1]; SPOT 1316: Mascot protein score=560. Number of peptides=6 [1]; SPOT 1323: Mascot protein score=50. Number of peptides=3 [1].
MAPPING (identification):
SPOT 1303: Tandem mass spectrometry [1];
SPOT 1306: Tandem mass spectrometry [1];
SPOT 1307: Tandem mass spectrometry [1];
SPOT 1309: Tandem mass spectrometry [1];
SPOT 1310: Tandem mass spectrometry [1];
SPOT 1316: Tandem mass spectrometry [1];
SPOT 1323: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP60711; ACTB_RAT.
World-2DPAGE RepositoryP60711; ACTB_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameACTB_RAT
Primary accession numberP60711
Secondary accession number(s) P02570 P70514 P99021 Q11211 Q64316
Sequence was last modified on April 1, 1988 (version 1)
Annotations were last modified on November 16, 2011 (version 79)
Name and origin of the protein
DescriptionRecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed;
Gene nameName=Actb
Encoded onName=Actb
KeywordsAcetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Methylation; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV01217; CAA24528.1; -; Genomic_DNA
EMBLBC063166; AAH63166.1; -; mRNA
IPIIPI00189819; -; .
RefSeqNP_112406.1; NM_031144.2; .
UniGeneRn.94978; -; .
ProteinModelPortalP60711; -; .
SMRP60711; 2-375; .
IntActP60711; 8; .
MINTMINT-132123; -; .
STRINGP60711; -; .
PhosphoSiteP60711; -; .
World-2DPAGE0004:P60711; -; .
PRIDEP60711; -; .
EnsemblENSRNOT00000042459; ENSRNOP00000044296; ENSRNOG00000034254; .
GeneID81822; -; .
KEGGrno:81822; -; .
UCSCNM_031144; rat; .
CTD60; -; .
RGD628837; Actb; .
eggNOGmaNOG08069; -; .
HOVERGENHBG003771; -; .
InParanoidP60711; -; .
OMAIKNLMER; -; .
OrthoDBEOG41JZC9; -; .
PhylomeDBP60711; -; .
ReactomeREACT_112697; Developmental Biology; .
NextBio615747; -; .
ArrayExpressP60711; -; .
GenevestigatorP60711; -; .
GermOnlineENSRNOG00000034254; Rattus norvegicus; .
GOGO:0030424; C:axon; IDA:RGD; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0019901; F:protein kinase binding; IPI:RGD; .
GOGO:0007409; P:axonogenesis; IDA:RGD; .
InterProIPR004000; Actin-like; .
InterProIPR020902; Actin/actin-like_CS; .
InterProIPR004001; Actin_CS; .
KOK05692; -; .
PANTHERPTHR11937; Actin_like; 1; .
PfamPF00022; Actin; 1; .
PRINTSPR00190; ACTIN; .
SMARTSM00268; ACTIN; 1; .
PROSITEPS00406; ACTINS_1; 1; .
PROSITEPS00432; ACTINS_2; 1; .
PROSITEPS01132; ACTINS_ACT_LIKE; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server