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Searching in 'World-2DPAGE Repository [0019]' for entry matching: AMYP_RAT

World-2DPAGE Repository (0019):  AMYP_RAT


General information about the entry
View entry in simple text format
Entry nameAMYP_RAT
Primary accession numberP00689
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Pancreatic alpha-amylase; Short=PA; EC=; AltName: Full=1,4-alpha-D-glucan glucanohydrolase; Flags: Precursor;.
Gene nameName=Amy2
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
  map experimental info

pI=8.19; Mw=57135  [identification data]

IDENTIFICATION: SPOT 759: Peptides number=2. SeqCov=5.5%. MOWSE score=1.38E+3 [1].
MAPPING (identification):
SPOT 759: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Data from Dr. Willard Freeman, Penn State College of Medicine, USA
UniProtKB/Swiss-ProtP00689; AMYP_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameAMYP_RAT
Primary accession numberP00689
Sequence was last modified on June 20, 2002 (version 2)
Annotations were last modified on December 14, 2011 (version 110)
Name and origin of the protein
DescriptionRecName: Full=Pancreatic alpha-amylase; Short=PA; EC=; AltName: Full=1,4-alpha-D-glucan glucanohydrolase; Flags: Precursor;
Gene nameName=Amy2
Encoded onName=Amy2
KeywordsCalcium; Carbohydrate metabolism; Chloride; Complete proteome; Disulfide bond; Glycosidase; Hydrolase; Metal-binding; Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLJ00703; AAA40725.2; -; mRNA
IPIIPI00211904; -; .
PIRA00840; ALRTP; .
RefSeqNP_113690.1; NM_031502.1; .
UniGeneRn.67070; -; .
ProteinModelPortalP00689; -; .
SMRP00689; 16-508; .
IntActP00689; 1; .
STRINGP00689; -; .
CAZyGH13; Glycoside Hydrolase Family 13; .
PhosphoSiteP00689; -; .
PRIDEP00689; -; .
GeneID497039; -; .
KEGGrno:497039; -; .
CTD497039; -; .
RGD1593187; Amy2; .
eggNOGmaNOG06932; -; .
GeneTreeENSGT00390000002882; -; .
HOVERGENHBG000061; -; .
InParanoidP00689; -; .
OrthoDBEOG4RV2R1; -; .
NextBio697675; -; .
GenevestigatorP00689; -; .
GermOnlineENSRNOG00000030590; Rattus norvegicus; .
GOGO:0005615; C:extracellular space; ISS:UniProtKB; .
GOGO:0004556; F:alpha-amylase activity; ISS:UniProtKB; .
GOGO:0005509; F:calcium ion binding; ISS:UniProtKB; .
GOGO:0031404; F:chloride ion binding; ISS:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:UniProtKB; .
GOGO:0016052; P:carbohydrate catabolic process; ISS:UniProtKB; .
InterProIPR006048; A-amylase_b_C; .
InterProIPR015902; Alpha_amylase; .
InterProIPR006046; Glyco_hydro_13; .
InterProIPR013780; Glyco_hydro_13_b; .
InterProIPR006047; Glyco_hydro_13_cat_dom; .
InterProIPR006589; Glyco_hydro_13_sub_cat_dom; .
InterProIPR013781; Glyco_hydro_subgr_catalytic; .
InterProIPR017853; Glycoside_hydrolase_SF; .
Gene3DG3DSA:; Glyco_hydro_13_b; 1; .
Gene3DG3DSA:; Glyco_hydro_cat; 1; .
KOK01176; -; .
PANTHERPTHR10357; Alpha_amylase; 1; .
PfamPF00128; Alpha-amylase; 1; .
PfamPF02806; Alpha-amylase_C; 1; .
SMARTSM00642; Aamy; 1; .
SMARTSM00632; Aamy_C; 1; .
SUPFAMSSF51445; Glyco_hydro_cat; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server