Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 331:

pI=7.93; Mw=51000

SPOT 333:

pI=7.55; Mw=51000

MAPPING (identification):

SPOT 331: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 333: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	ATPA_RAT
Primary accession number	P15999
Secondary accession number(s)	Q6P753
Sequence was last modified on	December 6, 2005 (version 2)
Annotations were last modified on	November 16, 2011 (version 123)
Name and origin of the protein
Description	RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;
Gene name	Name=Atp5a1
Encoded on	Name=Atp5a1
Keywords	3D-structure; Acetylation; ATP synthesis; ATP-binding; Cell membrane; CF(1); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC061830; AAH61830.1; -; mRNA
EMBL	J05266; AAA40784.1; -; mRNA
EMBL	X56133; CAA39599.1; -; mRNA
IPI	IPI00396910; -; .
PIR	A35730; A35730; .
RefSeq	NP_075581.1; NM_023093.1; .
UniGene	Rn.40255; -; .
PDB	1MAB; X-ray; 2.80 A; A=44-553
PDB	2F43; X-ray; 3.00 A; A=44-553
PDBsum	1MAB; -; .
PDBsum	2F43; -; .
ProteinModelPortal	P15999; -; .
SMR	P15999; 66-545; .
IntAct	P15999; 3; .
MINT	MINT-4588311; -; .
STRING	P15999; -; .
PhosphoSite	P15999; -; .
Rat-heart-2DPAGE	P15999; -; .
World-2DPAGE	0004:P15999; -; .
PRIDE	P15999; -; .
GeneID	65262; -; .
KEGG	rno:65262; -; .
UCSC	NM_023093; rat; .
CTD	498; -; .
RGD	619993; Atp5a1; .
eggNOG	roNOG06697; -; .
GeneTree	ENSGT00550000074846; -; .
HOVERGEN	HBG001536; -; .
InParanoid	P15999; -; .
OrthoDB	EOG4ZCT48; -; .
PhylomeDB	P15999; -; .
NextBio	614213; -; .
PMAP-CutDB	P15999; -; .
ArrayExpress	P15999; -; .
Genevestigator	P15999; -; .
GermOnline	ENSRNOG00000017032; Rattus norvegicus; .
GO	GO:0000275; C:mitochondrial proton-transporting ATP synthase complex; catalytic core F(1); IDA:RGD
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IDA:RGD; .
GO	GO:0046961; F:proton-transporting ATPase activity; rotational mechanism; IEA:InterPro
GO	GO:0006172; P:ADP biosynthetic process; IDA:RGD; .
GO	GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro; .
GO	GO:0015986; P:ATP synthesis coupled proton transport; IEA:InterPro; .
InterPro	IPR020003; ATPase_a/bsu_AS; .
InterPro	IPR005294; ATPase_F1-cplx_asu; .
InterPro	IPR018118; ATPase_F1/A1-cplx_a/bsu_N; .
InterPro	IPR023366; ATPase_F1/A1-cplx_a_su_N; .
InterPro	IPR000793; ATPase_F1/V1/A1-cplx_a/bsu_C; .
InterPro	IPR004100; ATPase_F1/V1/A1-cplx_a/bsu_N; .
InterPro	IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd; .
Gene3D	G3DSA:2.40.30.20; G3DSA:2.40.30.20; 1; .
KO	K02132; -; .
Pfam	PF00006; ATP-synt_ab; 1; .
Pfam	PF00306; ATP-synt_ab_C; 1; .
Pfam	PF02874; ATP-synt_ab_N; 1; .
SUPFAM	SSF47917; ATPase_a/b_C; 1; .
SUPFAM	SSF50615; ATPase_a/b_N; 1; .
TIGRFAMs	TIGR00962; AtpA; 1; .
PROSITE	PS00152; ATPASE_ALPHA_BETA; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: ATPA_RAT

World-2DPAGE Repository (0014): ATPA_RAT

ATPA_RAT

General information about the entry

Entry name

ATPA_RAT

Primary accession number

P15999

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;.

Gene name

Name=Atp5a1

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2866:

pI=9.22; Mw=58000

IDENTIFICATION: SPOT 2866: SeqCov=52%. MascotPMF=209 [1].
MAPPING (identification):

SPOT 2866: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	ATPA_RAT
Primary accession number	P15999
Secondary accession number(s)	Q6P753
Sequence was last modified on	December 6, 2005 (version 2)
Annotations were last modified on	November 16, 2011 (version 123)
Name and origin of the protein
Description	RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;
Gene name	Name=Atp5a1
Encoded on	Name=Atp5a1
Keywords	3D-structure; Acetylation; ATP synthesis; ATP-binding; Cell membrane; CF(1); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC061830; AAH61830.1; -; mRNA
EMBL	J05266; AAA40784.1; -; mRNA
EMBL	X56133; CAA39599.1; -; mRNA
IPI	IPI00396910; -; .
PIR	A35730; A35730; .
RefSeq	NP_075581.1; NM_023093.1; .
UniGene	Rn.40255; -; .
PDB	1MAB; X-ray; 2.80 A; A=44-553
PDB	2F43; X-ray; 3.00 A; A=44-553
PDBsum	1MAB; -; .
PDBsum	2F43; -; .
ProteinModelPortal	P15999; -; .
SMR	P15999; 66-545; .
IntAct	P15999; 3; .
MINT	MINT-4588311; -; .
STRING	P15999; -; .
PhosphoSite	P15999; -; .
Rat-heart-2DPAGE	P15999; -; .
World-2DPAGE	0004:P15999; -; .
PRIDE	P15999; -; .
GeneID	65262; -; .
KEGG	rno:65262; -; .
UCSC	NM_023093; rat; .
CTD	498; -; .
RGD	619993; Atp5a1; .
eggNOG	roNOG06697; -; .
GeneTree	ENSGT00550000074846; -; .
HOVERGEN	HBG001536; -; .
InParanoid	P15999; -; .
OrthoDB	EOG4ZCT48; -; .
PhylomeDB	P15999; -; .
NextBio	614213; -; .
PMAP-CutDB	P15999; -; .
ArrayExpress	P15999; -; .
Genevestigator	P15999; -; .
GermOnline	ENSRNOG00000017032; Rattus norvegicus; .
GO	GO:0000275; C:mitochondrial proton-transporting ATP synthase complex; catalytic core F(1); IDA:RGD
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IDA:RGD; .
GO	GO:0046961; F:proton-transporting ATPase activity; rotational mechanism; IEA:InterPro
GO	GO:0006172; P:ADP biosynthetic process; IDA:RGD; .
GO	GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro; .
GO	GO:0015986; P:ATP synthesis coupled proton transport; IEA:InterPro; .
InterPro	IPR020003; ATPase_a/bsu_AS; .
InterPro	IPR005294; ATPase_F1-cplx_asu; .
InterPro	IPR018118; ATPase_F1/A1-cplx_a/bsu_N; .
InterPro	IPR023366; ATPase_F1/A1-cplx_a_su_N; .
InterPro	IPR000793; ATPase_F1/V1/A1-cplx_a/bsu_C; .
InterPro	IPR004100; ATPase_F1/V1/A1-cplx_a/bsu_N; .
InterPro	IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd; .
Gene3D	G3DSA:2.40.30.20; G3DSA:2.40.30.20; 1; .
KO	K02132; -; .
Pfam	PF00006; ATP-synt_ab; 1; .
Pfam	PF00306; ATP-synt_ab_C; 1; .
Pfam	PF02874; ATP-synt_ab_N; 1; .
SUPFAM	SSF47917; ATPase_a/b_C; 1; .
SUPFAM	SSF50615; ATPase_a/b_N; 1; .
TIGRFAMs	TIGR00962; AtpA; 1; .
PROSITE	PS00152; ATPASE_ALPHA_BETA; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: ATPA_RAT

World-2DPAGE Repository (0019): ATPA_RAT

ATPA_RAT

General information about the entry

Entry name

ATPA_RAT

Primary accession number

P15999

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;.

Gene name

Name=Atp5a1

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 411:

pI=8.28; Mw=55361

SPOT 420:

pI=9.22; Mw=58904

SPOT 422:

pI=8.28; Mw=55361

SPOT 438:

pI=4.95; Mw=51320

SPOT 824:

pI=7.03; Mw=25639

SPOT 864:

pI=7.03; Mw=25639

SPOT 1161:

pI=8.28; Mw=55361

IDENTIFICATION: SPOT 411: Peptides number=9. SeqCov=21.7%. MOWSE score=8.35E+8 [1]
SPOT 420: Peptides number=5. SeqCov=11.9%. MOWSE score=5.50E+4 [1]; SPOT 422: Peptides number=12. SeqCov=23.9%. MOWSE score=2.43E+8 [1]; SPOT 438: Peptides number=10. SeqCov=28.7%. MOWSE score=1.25E+6 [1]; SPOT 824: Peptides number=5. SeqCov=15.4%. MOWSE score=2.50E+4 [1]; SPOT 864: Peptides number=4. SeqCov=12.8%. MOWSE score=5.37E+4 [1]; SPOT 1161: Peptides number=10. SeqCov=23%. MOWSE score=1.96E+9 [1].
MAPPING (identification):

SPOT 411: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 420: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 422: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 438: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 824: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 864: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1161: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	ATPA_RAT
Primary accession number	P15999
Secondary accession number(s)	Q6P753
Sequence was last modified on	December 6, 2005 (version 2)
Annotations were last modified on	November 16, 2011 (version 123)
Name and origin of the protein
Description	RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;
Gene name	Name=Atp5a1
Encoded on	Name=Atp5a1
Keywords	3D-structure; Acetylation; ATP synthesis; ATP-binding; Cell membrane; CF(1); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC061830; AAH61830.1; -; mRNA
EMBL	J05266; AAA40784.1; -; mRNA
EMBL	X56133; CAA39599.1; -; mRNA
IPI	IPI00396910; -; .
PIR	A35730; A35730; .
RefSeq	NP_075581.1; NM_023093.1; .
UniGene	Rn.40255; -; .
PDB	1MAB; X-ray; 2.80 A; A=44-553
PDB	2F43; X-ray; 3.00 A; A=44-553
PDBsum	1MAB; -; .
PDBsum	2F43; -; .
ProteinModelPortal	P15999; -; .
SMR	P15999; 66-545; .
IntAct	P15999; 3; .
MINT	MINT-4588311; -; .
STRING	P15999; -; .
PhosphoSite	P15999; -; .
Rat-heart-2DPAGE	P15999; -; .
World-2DPAGE	0004:P15999; -; .
PRIDE	P15999; -; .
GeneID	65262; -; .
KEGG	rno:65262; -; .
UCSC	NM_023093; rat; .
CTD	498; -; .
RGD	619993; Atp5a1; .
eggNOG	roNOG06697; -; .
GeneTree	ENSGT00550000074846; -; .
HOVERGEN	HBG001536; -; .
InParanoid	P15999; -; .
OrthoDB	EOG4ZCT48; -; .
PhylomeDB	P15999; -; .
NextBio	614213; -; .
PMAP-CutDB	P15999; -; .
ArrayExpress	P15999; -; .
Genevestigator	P15999; -; .
GermOnline	ENSRNOG00000017032; Rattus norvegicus; .
GO	GO:0000275; C:mitochondrial proton-transporting ATP synthase complex; catalytic core F(1); IDA:RGD
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IDA:RGD; .
GO	GO:0046961; F:proton-transporting ATPase activity; rotational mechanism; IEA:InterPro
GO	GO:0006172; P:ADP biosynthetic process; IDA:RGD; .
GO	GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro; .
GO	GO:0015986; P:ATP synthesis coupled proton transport; IEA:InterPro; .
InterPro	IPR020003; ATPase_a/bsu_AS; .
InterPro	IPR005294; ATPase_F1-cplx_asu; .
InterPro	IPR018118; ATPase_F1/A1-cplx_a/bsu_N; .
InterPro	IPR023366; ATPase_F1/A1-cplx_a_su_N; .
InterPro	IPR000793; ATPase_F1/V1/A1-cplx_a/bsu_C; .
InterPro	IPR004100; ATPase_F1/V1/A1-cplx_a/bsu_N; .
InterPro	IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd; .
Gene3D	G3DSA:2.40.30.20; G3DSA:2.40.30.20; 1; .
KO	K02132; -; .
Pfam	PF00006; ATP-synt_ab; 1; .
Pfam	PF00306; ATP-synt_ab_C; 1; .
Pfam	PF02874; ATP-synt_ab_N; 1; .
SUPFAM	SSF47917; ATPase_a/b_C; 1; .
SUPFAM	SSF50615; ATPase_a/b_N; 1; .
TIGRFAMs	TIGR00962; AtpA; 1; .
PROSITE	PS00152; ATPASE_ALPHA_BETA; 1; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: ATPA_RAT

World-2DPAGE Repository (0022): ATPA_RAT

ATPA_RAT

General information about the entry

Entry name

ATPA_RAT

Primary accession number

P15999

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;.

Gene name

Name=Atp5a1

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 164:

pI=9.22; Mw=59831

SPOT 691:

pI=9.22; Mw=59831

SPOT 735:

pI=9.22; Mw=59831

SPOT 853:

pI=9.22; Mw=59831

SPOT 865:

pI=9.22; Mw=59831

IDENTIFICATION: SPOT 164: SeqCov=36%. Peptides MS=17/20. Mascot PMF Score=172 [1]
SPOT 691: SeqCov=29.5%. Peptides MS=14/20. Mascot PMF Score=112 [1]; SPOT 735: SeqCov=36%. Peptides MS=17/20. Mascot PMF Score=172 [1]; SPOT 853: SeqCov=21.2%. Peptides MS=7/20. Mascot PMF Score=65 [1]; SPOT 865: SeqCov=29.3%. Peptides MS=11/20. Mascot PMF Score=109 [1].
PEPTIDE EVIDENCE: SPOT 691: 335-EAYPGDVFYLHSR-347 (66) / 473-QGQYSPMAIEEQVAVIYAGVR-493 (83) / 442-EVAAFAQFGSDLDAATQQLLSR-463 (78) [1]
SPOT 853: 435-LELAQYR-441 (29) / 473-QGQYSPMAIEEQVAVIYAGVR-493 (122) / 442-EVAAFAQFGSDLDAATQQLLSR-463 (88) [1]; SPOT 865: 335-EAYPGDVFYLHSR-347 (81) / 473-QGQYSPMAIEEQVAVIYAGVR-493 (32) / 442-EVAAFAQFGSDLDAATQQLLSR-463 (86) [1].
MAPPING (identification):

SPOT 164: Peptide mass fingerprinting [1];
SPOT 691: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 735: Peptide mass fingerprinting [1];
SPOT 853: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 865: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	ATPA_RAT
Primary accession number	P15999
Secondary accession number(s)	Q6P753
Sequence was last modified on	December 6, 2005 (version 2)
Annotations were last modified on	November 16, 2011 (version 123)
Name and origin of the protein
Description	RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;
Gene name	Name=Atp5a1
Encoded on	Name=Atp5a1
Keywords	3D-structure; Acetylation; ATP synthesis; ATP-binding; Cell membrane; CF(1); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC061830; AAH61830.1; -; mRNA
EMBL	J05266; AAA40784.1; -; mRNA
EMBL	X56133; CAA39599.1; -; mRNA
IPI	IPI00396910; -; .
PIR	A35730; A35730; .
RefSeq	NP_075581.1; NM_023093.1; .
UniGene	Rn.40255; -; .
PDB	1MAB; X-ray; 2.80 A; A=44-553
PDB	2F43; X-ray; 3.00 A; A=44-553
PDBsum	1MAB; -; .
PDBsum	2F43; -; .
ProteinModelPortal	P15999; -; .
SMR	P15999; 66-545; .
IntAct	P15999; 3; .
MINT	MINT-4588311; -; .
STRING	P15999; -; .
PhosphoSite	P15999; -; .
Rat-heart-2DPAGE	P15999; -; .
World-2DPAGE	0004:P15999; -; .
PRIDE	P15999; -; .
GeneID	65262; -; .
KEGG	rno:65262; -; .
UCSC	NM_023093; rat; .
CTD	498; -; .
RGD	619993; Atp5a1; .
eggNOG	roNOG06697; -; .
GeneTree	ENSGT00550000074846; -; .
HOVERGEN	HBG001536; -; .
InParanoid	P15999; -; .
OrthoDB	EOG4ZCT48; -; .
PhylomeDB	P15999; -; .
NextBio	614213; -; .
PMAP-CutDB	P15999; -; .
ArrayExpress	P15999; -; .
Genevestigator	P15999; -; .
GermOnline	ENSRNOG00000017032; Rattus norvegicus; .
GO	GO:0000275; C:mitochondrial proton-transporting ATP synthase complex; catalytic core F(1); IDA:RGD
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IDA:RGD; .
GO	GO:0046961; F:proton-transporting ATPase activity; rotational mechanism; IEA:InterPro
GO	GO:0006172; P:ADP biosynthetic process; IDA:RGD; .
GO	GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro; .
GO	GO:0015986; P:ATP synthesis coupled proton transport; IEA:InterPro; .
InterPro	IPR020003; ATPase_a/bsu_AS; .
InterPro	IPR005294; ATPase_F1-cplx_asu; .
InterPro	IPR018118; ATPase_F1/A1-cplx_a/bsu_N; .
InterPro	IPR023366; ATPase_F1/A1-cplx_a_su_N; .
InterPro	IPR000793; ATPase_F1/V1/A1-cplx_a/bsu_C; .
InterPro	IPR004100; ATPase_F1/V1/A1-cplx_a/bsu_N; .
InterPro	IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd; .
Gene3D	G3DSA:2.40.30.20; G3DSA:2.40.30.20; 1; .
KO	K02132; -; .
Pfam	PF00006; ATP-synt_ab; 1; .
Pfam	PF00306; ATP-synt_ab_C; 1; .
Pfam	PF02874; ATP-synt_ab_N; 1; .
SUPFAM	SSF47917; ATPase_a/b_C; 1; .
SUPFAM	SSF50615; ATPase_a/b_N; 1; .
TIGRFAMs	TIGR00962; AtpA; 1; .
PROSITE	PS00152; ATPASE_ALPHA_BETA; 1; .

Searching in 'World-2DPAGE Repository [0030]' for entry matching: ATPA_RAT

World-2DPAGE Repository (0030): ATPA_RAT

ATPA_RAT

General information about the entry

Entry name

ATPA_RAT

Primary accession number

P15999

integrated into World-2DPAGE Repository (0030) on

November 9, 2010 (release 1)

2D Annotations were last modified on

June 21, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;.

Gene name

Name=Atp5a1

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011)

2D PAGE maps for identified proteins

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)

RAT_INS1E_4-7

MAP LOCATIONS:

SPOT 2348:

pI=5.80; Mw=23315

IDENTIFICATION: SPOT 2348: Mascot protein score=72. Number of peptides=1 [1].
MAPPING (identification):

SPOT 2348: Tandem mass spectrometry [1].

Copyright

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium

Cross-references

UniProtKB/Swiss-Prot

World-2DPAGE Repository

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	ATPA_RAT
Primary accession number	P15999
Secondary accession number(s)	Q6P753
Sequence was last modified on	December 6, 2005 (version 2)
Annotations were last modified on	November 16, 2011 (version 123)
Name and origin of the protein
Description	RecName: Full=ATP synthase subunit alpha, mitochondrial; Flags: Precursor;
Gene name	Name=Atp5a1
Encoded on	Name=Atp5a1
Keywords	3D-structure; Acetylation; ATP synthesis; ATP-binding; Cell membrane; CF(1); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC061830; AAH61830.1; -; mRNA
EMBL	J05266; AAA40784.1; -; mRNA
EMBL	X56133; CAA39599.1; -; mRNA
IPI	IPI00396910; -; .
PIR	A35730; A35730; .
RefSeq	NP_075581.1; NM_023093.1; .
UniGene	Rn.40255; -; .
PDB	1MAB; X-ray; 2.80 A; A=44-553
PDB	2F43; X-ray; 3.00 A; A=44-553
PDBsum	1MAB; -; .
PDBsum	2F43; -; .
ProteinModelPortal	P15999; -; .
SMR	P15999; 66-545; .
IntAct	P15999; 3; .
MINT	MINT-4588311; -; .
STRING	P15999; -; .
PhosphoSite	P15999; -; .
Rat-heart-2DPAGE	P15999; -; .
World-2DPAGE	0004:P15999; -; .
PRIDE	P15999; -; .
GeneID	65262; -; .
KEGG	rno:65262; -; .
UCSC	NM_023093; rat; .
CTD	498; -; .
RGD	619993; Atp5a1; .
eggNOG	roNOG06697; -; .
GeneTree	ENSGT00550000074846; -; .
HOVERGEN	HBG001536; -; .
InParanoid	P15999; -; .
OrthoDB	EOG4ZCT48; -; .
PhylomeDB	P15999; -; .
NextBio	614213; -; .
PMAP-CutDB	P15999; -; .
ArrayExpress	P15999; -; .
Genevestigator	P15999; -; .
GermOnline	ENSRNOG00000017032; Rattus norvegicus; .
GO	GO:0000275; C:mitochondrial proton-transporting ATP synthase complex; catalytic core F(1); IDA:RGD
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IDA:RGD; .
GO	GO:0046961; F:proton-transporting ATPase activity; rotational mechanism; IEA:InterPro
GO	GO:0006172; P:ADP biosynthetic process; IDA:RGD; .
GO	GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro; .
GO	GO:0015986; P:ATP synthesis coupled proton transport; IEA:InterPro; .
InterPro	IPR020003; ATPase_a/bsu_AS; .
InterPro	IPR005294; ATPase_F1-cplx_asu; .
InterPro	IPR018118; ATPase_F1/A1-cplx_a/bsu_N; .
InterPro	IPR023366; ATPase_F1/A1-cplx_a_su_N; .
InterPro	IPR000793; ATPase_F1/V1/A1-cplx_a/bsu_C; .
InterPro	IPR004100; ATPase_F1/V1/A1-cplx_a/bsu_N; .
InterPro	IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd; .
Gene3D	G3DSA:2.40.30.20; G3DSA:2.40.30.20; 1; .
KO	K02132; -; .
Pfam	PF00006; ATP-synt_ab; 1; .
Pfam	PF00306; ATP-synt_ab_C; 1; .
Pfam	PF02874; ATP-synt_ab_N; 1; .
SUPFAM	SSF47917; ATPase_a/b_C; 1; .
SUPFAM	SSF50615; ATPase_a/b_N; 1; .
TIGRFAMs	TIGR00962; AtpA; 1; .
PROSITE	PS00152; ATPASE_ALPHA_BETA; 1; .

Searching in 'World-2DPAGE Repository [0069]' for entry matching: ATPA_RAT

World-2DPAGE Repository (0069): ATPA_RAT

ATPA_RAT

General information about the entry

Entry name

ATPA_RAT

Primary accession number

ATPA_RAT

integrated into World-2DPAGE Repository (0069) on

February 27, 2014 (release 1)

2D Annotations were last modified on

February 27, 2014 (version 1)

General Annotations were last modified on

February 27, 2014 (version 1)

Name and origin of the protein

Description

ATPA_RAT.

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D GEL CHARACTERIZATION
DOI=10.1016/j.jprot.2014.04.015;
Burniston J.G., Kenyani J., Gray D., Guadagnin E., Jarman I.H., Cobley J.N., Cuthbertson D.J., Chen Y.-W., Wastling J.M., Lisboa P., Koch L.G., Britton S.L.
''Conditional independence mapping of DIGE data reveals PDIA3 protein species as key nodes associated with muscle aerobic capacity''
Journal of Proteomics 106(1):230-245 (2014)

2D PAGE maps for identified proteins

RATTUS_SOLEUS-DIGE_4.5-9.5 {M+F HCR LCR Soleus DiGE Exp}
Rattus
Tissue: Skeletal muscle