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Searching in 'World-2DPAGE Repository [0030]' for entry matching: CBPE_RAT




World-2DPAGE Repository (0030):  CBPE_RAT


CBPE_RAT


General information about the entry
View entry in simple text format
Entry nameCBPE_RAT
Primary accession numberP15087
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Carboxypeptidase E; Short=CPE; EC=3.4.17.10; AltName: Full=Carboxypeptidase H; Short=CPH; AltName: Full=Enkephalin convertase; AltName: Full=Prohormone-processing carboxypeptidase; Flags: Precursor;.
Gene nameName=Cpe
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=4.65; Mw=66902  [identification data]
pI=4.68; Mw=67087  [identification data]
pI=4.65; Mw=64807  [identification data]
pI=4.73; Mw=64538  [identification data]
pI=4.78; Mw=64449  [identification data]

IDENTIFICATION: SPOT 857: Mascot protein score=35. Number of peptides=1 [1]
SPOT 859: Mascot protein score=67. Number of peptides=7 [1]; SPOT 938: Mascot protein score=74. Number of peptides=5 [1]; SPOT 948: Mascot protein score=288. Number of peptides=7 [1]; SPOT 949: Mascot protein score=584. Number of peptides=8 [1].
MAPPING (identification):
SPOT 857: Tandem mass spectrometry [1];
SPOT 859: Tandem mass spectrometry [1];
SPOT 938: Tandem mass spectrometry [1];
SPOT 948: Tandem mass spectrometry [1];
SPOT 949: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP15087; CBPE_RAT.
World-2DPAGE RepositoryP15087; CBPE_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameCBPE_RAT
Primary accession numberP15087
Sequence was last modified on April 1, 1990 (version 1)
Annotations were last modified on November 16, 2011 (version 95)
Name and origin of the protein
DescriptionRecName: Full=Carboxypeptidase E; Short=CPE; EC=3.4.17.10; AltName: Full=Carboxypeptidase H; Short=CPH; AltName: Full=Enkephalin convertase; AltName: Full=Prohormone-processing carboxypeptidase; Flags: Precursor;
Gene nameName=Cpe
Encoded onName=Cpe
KeywordsCarboxypeptidase; Cleavage on pair of basic residues; Complete proteome; Cytoplasmic vesicle; Glycoprotein; Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome; Signal; Zinc; Zymogen.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ04625; AAA40875.1; -; mRNA
EMBLM31602; AAA40873.1; -; mRNA
EMBLX51406; CAA35768.1; -; mRNA
EMBLL07281; AAA40957.1; ALT_SEQ; Genomic_DNA
EMBLL07273; AAA40957.1; JOINED; Genomic_DNA
EMBLL07274; AAA40957.1; JOINED; Genomic_DNA
EMBLL07275; AAA40957.1; JOINED; Genomic_DNA
EMBLL07277; AAA40957.1; JOINED; Genomic_DNA
EMBLL07278; AAA40957.1; JOINED; Genomic_DNA
EMBLL07279; AAA40957.1; JOINED; Genomic_DNA
EMBLL07280; AAA40957.1; JOINED; Genomic_DNA
IPIIPI00326211; -; .
PIRA40469; A40469; .
PIRS12461; S12461; .
RefSeqNP_037260.1; NM_013128.1; .
UniGeneRn.7149; -; .
ProteinModelPortalP15087; -; .
SMRP15087; 49-451; .
STRINGP15087; -; .
MEROPSM14.005; -; .
GeneID25669; -; .
KEGGrno:25669; -; .
CTD1363; -; .
RGD2394; Cpe; .
HOVERGENHBG003410; -; .
NextBio607597; -; .
GenevestigatorP15087; -; .
GOGO:0030425; C:dendrite; IDA:BHF-UCL; .
GOGO:0005615; C:extracellular space; IDA:RGD; .
GOGO:0005794; C:Golgi apparatus; IDA:BHF-UCL; .
GOGO:0005624; C:membrane fraction; IDA:RGD; .
GOGO:0043204; C:perikaryon; IDA:BHF-UCL; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0030141; C:stored secretory granule; IDA:RGD; .
GOGO:0030133; C:transport vesicle; IEA:UniProtKB-SubCell; .
GOGO:0050839; F:cell adhesion molecule binding; IPI:BHF-UCL; .
GOGO:0050897; F:cobalt ion binding; IMP:RGD; .
GOGO:0004181; F:metallocarboxypeptidase activity; IEA:InterPro; .
GOGO:0042043; F:neurexin family protein binding; IPI:BHF-UCL; .
GOGO:0008270; F:zinc ion binding; IEA:InterPro; .
GOGO:0030070; P:insulin processing; IDA:RGD; .
GOGO:0006518; P:peptide metabolic process; IDA:RGD; .
GOGO:0006508; P:proteolysis; IEA:UniProtKB-KW; .
InterProIPR008969; CarboxyPept-like_regulatory; .
InterProIPR014766; CarboxyPept_regulatory_dom; .
InterProIPR000834; Peptidase_M14; .
Gene3DG3DSA:2.60.40.1120; CarboxyPept_regulatory; 1; .
KOK01294; -; .
PfamPF00246; Peptidase_M14; 1; .
PRINTSPR00765; CRBOXYPTASEA; .
SMARTSM00631; Zn_pept; 1; .
SUPFAMSSF49464; CarboxypepD_reg; 1; .
PROSITEPS00132; CARBOXYPEPT_ZN_1; 1; .
PROSITEPS00133; CARBOXYPEPT_ZN_2; 1; .



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