resource logo

World-2DPAGE Repository

Attention: World-2DPAGE is no longer maintained.

World-2DPAGE Repository no longer accepts submissions.


World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Databases

use 'Ctrl' to select several
databases


Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal

Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0030]' for entry matching: CMGA_RAT




World-2DPAGE Repository (0030):  CMGA_RAT


CMGA_RAT


General information about the entry
View entry in simple text format
Entry nameCMGA_RAT
Primary accession numberP10354
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Chromogranin-A; Short=CgA; Contains: RecName: Full=Pancreastatin; Contains: RecName: Full=Beta-granin; Contains: RecName: Full=WE-14; Flags: Precursor;.
Gene nameName=Chga
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=4.44; Mw=85463  [identification data]
pI=4.46; Mw=85463  [identification data]
pI=4.48; Mw=85345  [identification data]
pI=4.51; Mw=84991  [identification data]
pI=4.46; Mw=19024  [identification data]
pI=4.59; Mw=17827  [identification data]

IDENTIFICATION: SPOT 435: Mascot protein score=122. Number of peptides=2 [1]
SPOT 439: Mascot protein score=119. Number of peptides=5 [1]; SPOT 440: Mascot protein score=135. Number of peptides=5 [1]; SPOT 441: Mascot protein score=57. Number of peptides=4 [1]; SPOT 2547: Mascot protein score=59. Number of peptides=2 [1]; SPOT 2625: Mascot protein score=263. Number of peptides=3 [1].
MAPPING (identification):
SPOT 435: Tandem mass spectrometry [1];
SPOT 439: Tandem mass spectrometry [1];
SPOT 440: Tandem mass spectrometry [1];
SPOT 441: Tandem mass spectrometry [1];
SPOT 2547: Tandem mass spectrometry [1];
SPOT 2625: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP10354; CMGA_RAT.
World-2DPAGE RepositoryP10354; CMGA_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameCMGA_RAT
Primary accession numberP10354
Sequence was last modified on July 1, 1989 (version 1)
Annotations were last modified on September 21, 2011 (version 81)
Name and origin of the protein
DescriptionRecName: Full=Chromogranin-A; Short=CgA; Contains: RecName: Full=Pancreastatin; Contains: RecName: Full=Beta-granin; Contains: RecName: Full=WE-14; Flags: Precursor;
Gene nameName=Chga
Encoded onName=Chga
KeywordsAmidation; Calcium; Cleavage on pair of basic residues; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Phosphoprotein; Reference proteome; Secreted; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX06832; CAA29988.1; -; mRNA
IPIIPI00189627; -; .
PIRA23996; A23996; .
UniGeneRn.41024; -; .
STRINGP10354; -; .
PhosphoSiteP10354; -; .
PRIDEP10354; -; .
RGD2338; Chga; .
eggNOGroNOG16074; -; .
HOVERGENHBG001272; -; .
OrthoDBEOG4CZBGF; -; .
GenevestigatorP10354; -; .
GOGO:0005576; C:extracellular region; IEA:UniProtKB-SubCell; .
GOGO:0030141; C:stored secretory granule; IEA:InterPro; .
GOGO:0006996; P:organelle organization; IMP:RGD; .
InterProIPR001819; Chromogranin_AB; .
InterProIPR018054; Chromogranin_CS; .
InterProIPR001990; Granin; .
PANTHERPTHR10583; Chromogranin_AB; 1; .
PfamPF01271; Granin; 2; .
PRINTSPR00659; CHROMOGRANIN; .
PROSITEPS00422; GRANINS_1; 1; .
PROSITEPS00423; GRANINS_2; 1; .



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server