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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: COMT_RAT World-2DPAGE Repository (0030):  COMT_RAT COMT_RAT General information about the entry View entry in simple text format Entry name COMT_RAT Primary accession number P22734 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Catechol O-methyltransferase; EC=2.1.1.6;. Gene name Name=Comt Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 2261: pI=4.67; Mw=25054   [identification data] SPOT 2296: pI=4.81; Mw=24303   [identification data] IDENTIFICATION: SPOT 2261: Mascot protein score=65. Number of peptides=3 [1] SPOT 2296: Mascot protein score=321. Number of peptides=7 [1]. MAPPING (identification): SPOT 2261: Tandem mass spectrometry [1]; SPOT 2296: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot P22734; COMT_RAT. World-2DPAGE Repository P22734; COMT_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name COMT_RAT Primary accession number P22734 Sequence was last modified on  May 1, 1992 (version 2) Annotations were last modified on  November 16, 2011 (version 127) Name and origin of the protein Description RecName: Full=Catechol O-methyltransferase; EC=2.1.1.6; Gene name Name=Comt Encoded on Name=Comt Keywords 3D-structure; Alternative initiation; Catecholamine metabolism; Cell membrane; Complete proteome; Cytoplasm; Magnesium; Membrane; Metal-binding; Methyltransferase; Neurotransmitter degradation; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL Z12651; CAA78276.1; -; Genomic_DNA EMBL M60754; AAA40882.1; ALT_INIT; Genomic_DNA EMBL BC081850; AAH81850.1; -; mRNA EMBL M60753; AAA40881.1; ALT_INIT; mRNA IPI IPI00210280; -; . IPI IPI00760117; -; . PIR S22090; S22090; . RefSeq NP_036663.1; NM_012531.2; . UniGene Rn.220; -; . PDB 1H1D; X-ray; 2.00 A; A=44-264 PDB 1JR4; X-ray; 2.63 A; A=44-264 PDB 1VID; X-ray; 2.00 A; A=44-264 PDB 2CL5; X-ray; 1.60 A; A/B=44-264 PDB 2ZLB; X-ray; 2.20 A; A=44-264 PDB 2ZTH; X-ray; 2.60 A; A=44-264 PDB 2ZVJ; X-ray; 2.30 A; A=44-264 PDB 3A7D; X-ray; 2.40 A; A=44-264 PDB 3HVH; X-ray; 1.30 A; A=44-264 PDB 3HVI; X-ray; 1.20 A; A=44-264 PDB 3HVJ; X-ray; 1.79 A; A/B=44-264 PDB 3HVK; X-ray; 1.30 A; A=44-264 PDB 3NW9; X-ray; 1.65 A; A=44-264 PDB 3NWB; X-ray; 1.30 A; A=44-264 PDB 3NWE; X-ray; 1.50 A; A=44-264 PDB 3OE4; X-ray; 1.49 A; A=44-264 PDB 3OE5; X-ray; 1.52 A; A=44-264 PDB 3OZR; X-ray; 1.73 A; A=44-264 PDB 3OZS; X-ray; 1.44 A; A=44-264 PDB 3OZT; X-ray; 1.48 A; A=44-264 PDBsum 1H1D; -; . PDBsum 1JR4; -; . PDBsum 1VID; -; . PDBsum 2CL5; -; . PDBsum 2ZLB; -; . PDBsum 2ZTH; -; . PDBsum 2ZVJ; -; . PDBsum 3A7D; -; . PDBsum 3HVH; -; . PDBsum 3HVI; -; . PDBsum 3HVJ; -; . PDBsum 3HVK; -; . PDBsum 3NW9; -; . PDBsum 3NWB; -; . PDBsum 3NWE; -; . PDBsum 3OE4; -; . PDBsum 3OE5; -; . PDBsum 3OZR; -; . PDBsum 3OZS; -; . PDBsum 3OZT; -; . ProteinModelPortal P22734; -; . SMR P22734; 45-258; . STRING P22734; -; . PhosphoSite P22734; -; . PRIDE P22734; -; . Ensembl ENSRNOT00000050269; ENSRNOP00000043148; ENSRNOG00000001889; . GeneID 24267; -; . KEGG rno:24267; -; . UCSC BC081850; rat; . CTD 1312; -; . RGD 2379; Comt; . eggNOG roNOG14769; -; . GeneTree ENSGT00390000011316; -; . HOVERGEN HBG005376; -; . InParanoid P22734; -; . OMA SRFECTH; -; . OrthoDB EOG4G1MH8; -; . PhylomeDB P22734; -; . BioCyc MetaCyc:MONOMER-15100; -; . NextBio 602825; -; . ArrayExpress P22734; -; . Genevestigator P22734; -; . GermOnline ENSRNOG00000001889; Rattus norvegicus; . GO GO:0016021; C:integral to membrane; IEA:UniProtKB-KW; . GO GO:0005624; C:membrane fraction; IDA:RGD; . GO GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; . GO GO:0005625; C:soluble fraction; IDA:RGD; . GO GO:0016206; F:catechol O-methyltransferase activity; IDA:RGD; . GO GO:0000287; F:magnesium ion binding; TAS:RGD; . GO GO:0008210; P:estrogen metabolic process; IMP:RGD; . GO GO:0007565; P:female pregnancy; IMP:RGD; . GO GO:0007612; P:learning; IMP:RGD; . GO GO:0048609; P:multicellular organismal reproductive process; IMP:RGD; . GO GO:0045963; P:negative regulation of dopamine metabolic process; IMP:RGD; . GO GO:0048662; P:negative regulation of smooth muscle cell proliferation; IMP:RGD; . GO GO:0042135; P:neurotransmitter catabolic process; IEA:UniProtKB-KW; . GO GO:0050668; P:positive regulation of homocysteine metabolic process; IMP:RGD; . GO GO:0042493; P:response to drug; IEP:RGD; . GO GO:0032496; P:response to lipopolysaccharide; IEP:RGD; . GO GO:0014070; P:response to organic cyclic compound; IEP:RGD; . GO GO:0048265; P:response to pain; IMP:RGD; . GO GO:0046498; P:S-adenosylhomocysteine metabolic process; TAS:RGD; . GO GO:0046500; P:S-adenosylmethionine metabolic process; TAS:RGD; . InterPro IPR017128; Catechol_O-MeTrfase_euk; . InterPro IPR002935; O-MeTrfase_3; . KO K00545; -; . PANTHER PTHR10509; Methyltransf_3; 1; . Pfam PF01596; Methyltransf_3; 1; . PIRSF PIRSF037177; Catechol_O-mtfrase_euk; 1; . World-2DPAGE Repository (search AC)