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Searching in 'World-2DPAGE Repository [0022]' for entry matching: DHSA_RAT




World-2DPAGE Repository (0022):  DHSA_RAT

DHSA_RAT


General information about the entry
View entry in simple text format
Entry nameDHSA_RAT
Primary accession numberQ920L2
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor;.
Gene nameName=Sdha
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.75; Mw=72596  [identification data]
pI=6.75; Mw=72596  [identification data]
pI=6.75; Mw=72596  [identification data]
pI=6.75; Mw=72596  [identification data]

IDENTIFICATION: SPOT 506: SeqCov=18%. Peptides MS=8/20. Mascot PMF Score=54 [1]
SPOT 692: SeqCov=21%. Peptides MS=10/20. Mascot PMF Score=80 [1]; SPOT 771: SeqCov=22.3%. Peptides MS=11/20. Mascot PMF Score=91 [1]; SPOT 902: SeqCov=29.7%. Peptides MS=14/20. Mascot PMF Score=136 [1].
PEPTIDE EVIDENCE: SPOT 506: 629-VTLDYRPVIDK-639 (39) / 444-LGANSLLDLVVFGR-457 (50) [1]
SPOT 692: 444-LGANSLLDLVVFGR-457 (110) / 354-DHVYLQLHHLPPEQLATR-371 (50) / 39-VSDAISTQYPVVDHEFDAVVVGAGGAGLR-67 (43) [1]; SPOT 771: 608-KPFAEHWR-615 (36) / 629-VTLDYRPVIDK-639 (36) / 444-LGANSLLDLVVFGR-457 (86) [1].
MAPPING (identification):
SPOT 506: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 692: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 771: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 902: Peptide mass fingerprinting [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtQ920L2; DHSA_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameDHSA_RAT
Primary accession numberQ920L2
Sequence was last modified on December 1, 2001 (version 1)
Annotations were last modified on November 16, 2011 (version 85)
Name and origin of the protein
DescriptionRecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor;
Gene nameName=Sdha
Encoded onName=Sdha
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Transport; Tricarboxylic acid cycle; Tumor suppressor.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB072907; BAB69818.1; -; mRNA
IPIIPI00200659; -; .
RefSeqNP_569112.1; NM_130428.1; .
UniGeneRn.101725; -; .
ProteinModelPortalQ920L2; -; .
SMRQ920L2; 44-656; .
IntActQ920L2; 1; .
STRINGQ920L2; -; .
PhosphoSiteQ920L2; -; .
World-2DPAGE0004:Q920L2; -; .
PRIDEQ920L2; -; .
EnsemblENSRNOT00000018336; ENSRNOP00000018336; ENSRNOG00000013331; .
GeneID157074; -; .
KEGGrno:157074; -; .
NMPDRfig|10116.3.peg.337; -; .
UCSCNM_130428; rat; .
CTD6389; -; .
RGD621557; Sdha; .
GeneTreeENSGT00390000010046; -; .
HOVERGENHBG001461; -; .
PhylomeDBQ920L2; -; .
NextBio620967; -; .
ArrayExpressQ920L2; -; .
GenevestigatorQ920L2; -; .
GermOnlineENSRNOG00000013331; Rattus norvegicus; .
GOGO:0005749; C:mitochondrial respiratory chain complex II; ISS:UniProtKB; .
GOGO:0009055; F:electron carrier activity; IEA:InterPro; .
GOGO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro; .
GOGO:0008177; F:succinate dehydrogenase (ubiquinone) activity; IMP:RGD; .
GOGO:0022904; P:respiratory electron transport chain; ISS:UniProtKB; .
GOGO:0006105; P:succinate metabolic process; ISS:UniProtKB; .
GOGO:0006810; P:transport; IEA:UniProtKB-KW; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR003953; FAD_bind2_N; .
InterProIPR003952; FRD_SDH_FAD_BS; .
InterProIPR015939; Fum_Rdtase/Succ_DH_flav-like_C; .
InterProIPR004112; Fum_Rdtase/Succ_DH_flav_C; .
InterProIPR011281; Succ_DH_flav_su_fwd; .
InterProIPR014006; Succ_Dhase_FrdA_Gneg; .
Gene3DG3DSA:1.20.58.100; Fum_Rdtase/Succ_DH_flav-like_C; 1; .
KOK00234; -; .
PfamPF00890; FAD_binding_2; 1; .
PfamPF02910; Succ_DH_flav_C; 1; .
SUPFAMSSF46977; Succ_DH_flav_C; 1; .
TIGRFAMsTIGR01816; SdhA_forward; 1; .
TIGRFAMsTIGR01812; SdhA_frdA_Gneg; 1; .
PROSITEPS00504; FRD_SDH_FAD_BINDING; 1; .


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