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Searching in 'World-2DPAGE Repository [0030]' for entry matching: EF2_RAT

World-2DPAGE Repository (0030):  EF2_RAT


General information about the entry
View entry in simple text format
Entry nameEF2_RAT
Primary accession numberP05197
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor 2; Short=EF-2;.
Gene nameName=Eef2
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
  map experimental info

pI=6.66; Mw=92603  [identification data]
pI=6.51; Mw=60979  [identification data]
pI=5.93; Mw=47210  [identification data]
pI=6.13; Mw=46950  [identification data]

IDENTIFICATION: SPOT 293: Mascot protein score=277. Number of peptides=9 [1]
SPOT 1053: Mascot protein score=68. Number of peptides=3 [1]; SPOT 1448: Mascot protein score=424. Number of peptides=10 [1]; SPOT 1472: Mascot protein score=404. Number of peptides=12 [1].
MAPPING (identification):
SPOT 293: Tandem mass spectrometry [1];
SPOT 1053: Tandem mass spectrometry [1];
SPOT 1448: Tandem mass spectrometry [1];
SPOT 1472: Tandem mass spectrometry [1].

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
UniProtKB/Swiss-ProtP05197; EF2_RAT.
World-2DPAGE RepositoryP05197; EF2_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameEF2_RAT
Primary accession numberP05197
Secondary accession number(s) P97619
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on November 16, 2011 (version 110)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor 2; Short=EF-2;
Gene nameName=Eef2
Encoded onName=Eef2
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; GTP-binding; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Ubl conjugation.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLBC066661; AAH66661.1; -; mRNA
EMBLK03502; AAA41106.1; -; mRNA
EMBLY07504; CAA68805.1; -; mRNA
EMBLU75403; AAB19107.1; -; mRNA
EMBLAF000576; AAD05363.1; -; mRNA
IPIIPI00203214; -; .
PIRS04007; EFRT2; .
RefSeqNP_058941.1; NM_017245.2; .
UniGeneRn.55145; -; .
ProteinModelPortalP05197; -; .
SMRP05197; 1-858; .
IntActP05197; 1; .
MINTMINT-4568758; -; .
STRINGP05197; -; .
PhosphoSiteP05197; -; .
World-2DPAGE0004:P05197; -; .
PRIDEP05197; -; .
EnsemblENSRNOT00000047450; ENSRNOP00000041821; ENSRNOG00000020266; .
GeneID29565; -; .
KEGGrno:29565; -; .
UCSCNM_017245; rat; .
CTD1938; -; .
RGD61979; Eef2; .
eggNOGmaNOG11430; -; .
HOVERGENHBG001838; -; .
InParanoidP05197; -; .
OrthoDBEOG4GXFM2; -; .
PhylomeDBP05197; -; .
NextBio609632; -; .
ArrayExpressP05197; -; .
GenevestigatorP05197; -; .
GermOnlineENSRNOG00000020266; Rattus norvegicus; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0005525; F:GTP binding; IEA:UniProtKB-KW; .
GOGO:0003924; F:GTPase activity; IEA:InterPro; .
GOGO:0003746; F:translation elongation factor activity; TAS:RGD; .
InterProIPR009022; Elongation_fac_G/III/V; .
InterProIPR000795; ProtSyn_GTP-bd; .
InterProIPR020568; Ribosomal_S5_D2-typ_fold; .
InterProIPR014721; Ribosomal_S5_D2-typ_fold_subgr; .
InterProIPR005225; Small_GTP-bd_dom; .
InterProIPR000640; Transl_elong_EFG/EF2_C; .
InterProIPR005517; Transl_elong_EFG/EF2_IV; .
InterProIPR004161; Transl_elong_EFTu/EF1A_2; .
InterProIPR009000; Transl_elong_init/rib_B-barrel; .
Gene3DG3DSA:; Ribosomal_S5_D2-type_fold; 1; .
Gene3DG3DSA:; Transl_elong_EFG/EF2_C; 1; .
KOK03234; -; .
PfamPF00679; EFG_C; 1; .
PfamPF03764; EFG_IV; 1; .
PfamPF00009; GTP_EFTU; 1; .
PfamPF03144; GTP_EFTU_D2; 1; .
SMARTSM00838; EFG_C; 1; .
SMARTSM00889; EFG_IV; 1; .
SUPFAMSSF54211; Ribosomal_S5_D2-typ_fold; 1; .
SUPFAMSSF50447; Translat_factor; 1; .
TIGRFAMsTIGR00231; Small_GTP; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server