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Searching in 'World-2DPAGE Repository [0030]' for entry matching: G3P_RAT




World-2DPAGE Repository (0030):  G3P_RAT


G3P_RAT


General information about the entry
View entry in simple text format
Entry nameG3P_RAT
Primary accession numberP04797
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=38 kDa BFA-dependent ADP-ribosylation substrate; AltName: Full=BARS-38; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;.
Gene nameName=Gapdh
Synonyms=Gapd
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.61; Mw=45732  [identification data]
pI=6.65; Mw=45291  [identification data]

IDENTIFICATION: SPOT 1497: Mascot protein score=70. Number of peptides=2 [1]
SPOT 1528: Mascot protein score=103. Number of peptides=2 [1].
MAPPING (identification):
SPOT 1497: Tandem mass spectrometry [1];
SPOT 1528: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP04797; G3P_RAT.
World-2DPAGE RepositoryP04797; G3P_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameG3P_RAT
Primary accession numberP04797
Secondary accession number(s) P09328 Q5M916 Q9QWU4
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 125)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=38 kDa BFA-dependent ADP-ribosylation substrate; AltName: Full=BARS-38; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;
Gene nameName=Gapdh
Synonyms=Gapd
Encoded onName=Gapdh; Synonyms=Gapd
KeywordsAcetylation; ADP-ribosylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Methylation; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; S-nitrosylation; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX02231; CAA26150.1; -; mRNA
EMBLM17701; AAA41193.1; -; mRNA
EMBLAB017801; BAB11748.1; -; mRNA
EMBLAF106860; AAD08929.2; -; mRNA
EMBLBC059110; AAH59110.1; -; mRNA
EMBLBC087743; AAH87743.1; -; mRNA
EMBLM29341; AAA40814.1; -; mRNA
EMBLM11561; AAA41795.1; -; mRNA
IPIIPI00555252; -; .
PIRA23013; DERTG; .
RefSeqNP_058704.1; NM_017008.3; .
RefSeqXP_001062726.1; XM_001062726.2; .
RefSeqXP_002726600.1; XM_002726554.1; .
UniGeneRn.129558; -; .
UniGeneRn.91450; -; .
ProteinModelPortalP04797; -; .
SMRP04797; 2-333; .
IntActP04797; 3; .
MINTMINT-1775142; -; .
STRINGP04797; -; .
PhosphoSiteP04797; -; .
Rat-heart-2DPAGEP04797; -; .
World-2DPAGE0004:P04797; -; .
PRIDEP04797; -; .
EnsemblENSRNOT00000050443; ENSRNOP00000040878; ENSRNOG00000018630; .
GeneID24383; -; .
GeneID685186; -; .
KEGGrno:24383; -; .
KEGGrno:685186; -; .
UCSCNM_017008; rat; .
CTD2597; -; .
RGD2661; Gapdh; .
eggNOGmaNOG06292; -; .
HOVERGENHBG000227; -; .
InParanoidP04797; -; .
OrthoDBEOG4Q84XS; -; .
PhylomeDBP04797; -; .
NextBio603149; -; .
GenevestigatorP04797; -; .
GermOnlineENSRNOG00000033057; Rattus norvegicus; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005634; C:nucleus; IDA:UniProtKB; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:UniProtKB; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0035605; F:peptidyl-cysteine S-nitrosylase activity; IDA:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:UniProtKB; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0006096; P:glycolysis; IDA:RGD; .
GOGO:0051402; P:neuron apoptosis; IDA:UniProtKB; .
GOGO:0035606; P:peptidyl-cysteine S-trans-nitrosylation; IDA:UniProtKB; .
GOGO:0050821; P:protein stabilization; IDA:UniProtKB; .
InterProIPR020831; GlycerAld/Erythrose_P_DH; .
InterProIPR020830; GlycerAld_3-P_DH_AS; .
InterProIPR020829; GlycerAld_3-P_DH_cat; .
InterProIPR020828; GlycerAld_3-P_DH_NAD(P)-bd; .
InterProIPR006424; Glyceraldehyde-3-P_DH_1; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00134; -; .
PANTHERPTHR10836; GAP_DH; 1; .
PfamPF02800; Gp_dh_C; 1; .
PfamPF00044; Gp_dh_N; 1; .
PIRSFPIRSF000149; GAP_DH; 1; .
PRINTSPR00078; G3PDHDRGNASE; .
SMARTSM00846; Gp_dh_N; 1; .
TIGRFAMsTIGR01534; GAPDH-I; 1; .
PROSITEPS00071; GAPDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server