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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: GRP78_RAT World-2DPAGE Repository (0030):  GRP78_RAT GRP78_RAT General information about the entry View entry in simple text format Entry name GRP78_RAT Primary accession number P06761 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName: Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy chain-binding protein; Short=BiP; AltName: Full=Steroidogenesis-activator polypeptide; Flags: Precursor;. Gene name Name=Hspa5 Synonyms=Grp78 Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 499: pI=5.03; Mw=82102   [identification data] SPOT 509: pI=4.72; Mw=81536   [identification data] SPOT 510: pI=4.75; Mw=81649   [identification data] SPOT 515: pI=4.79; Mw=81536   [identification data] SPOT 516: pI=4.84; Mw=81424   [identification data] SPOT 519: pI=4.82; Mw=81424   [identification data] SPOT 525: pI=4.86; Mw=81086   [identification data] SPOT 529: pI=4.96; Mw=81086   [identification data] SPOT 531: pI=4.89; Mw=80862   [identification data] SPOT 1394: pI=5.61; Mw=49896   [identification data] SPOT 2988: pI=4.92; Mw=80416   [identification data] IDENTIFICATION: SPOT 499: Mascot protein score=895. Number of peptides=11 [1] SPOT 509: Mascot protein score=56. Number of peptides=4 [1]; SPOT 510: Mascot protein score=51. Number of peptides=4 [1]; SPOT 515: Mascot protein score=85. Number of peptides=7 [1]; SPOT 516: Mascot protein score=67. Number of peptides=4 [1]; SPOT 519: Mascot protein score=312. Number of peptides=9 [1]; SPOT 525: Mascot protein score=540. Number of peptides=14 [1]; SPOT 529: Mascot protein score=72. Number of peptides=5 [1]; SPOT 531: Mascot protein score=989. Number of peptides=15 [1]; SPOT 1394: Mascot protein score=293. Number of peptides=9 [1]; SPOT 2988: Mascot protein score=652. Number of peptides=9 [1]. MAPPING (identification): SPOT 499: Tandem mass spectrometry [1]; SPOT 509: Tandem mass spectrometry [1]; SPOT 510: Tandem mass spectrometry [1]; SPOT 515: Tandem mass spectrometry [1]; SPOT 516: Tandem mass spectrometry [1]; SPOT 519: Tandem mass spectrometry [1]; SPOT 525: Tandem mass spectrometry [1]; SPOT 529: Tandem mass spectrometry [1]; SPOT 531: Tandem mass spectrometry [1]; SPOT 1394: Tandem mass spectrometry [1]; SPOT 2988: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot P06761; GRP78_RAT. World-2DPAGE Repository P06761; GRP78_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name GRP78_RAT Primary accession number P06761 Sequence was last modified on  April 1, 1988 (version 1) Annotations were last modified on  November 16, 2011 (version 117) Name and origin of the protein Description RecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName: Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy chain-binding protein; Short=BiP; AltName: Full=Steroidogenesis-activator polypeptide; Flags: Precursor; Gene name Name=Hspa5 Synonyms=Grp78 Encoded on Name=Hspa5; Synonyms=Grp78 Keywords Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Nucleotide-binding; Phosphoprotein; Reference proteome; Signal. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M14050; AAA40817.1; -; mRNA EMBL BC062017; AAH62017.1; -; mRNA EMBL M14866; AAA41277.1; -; Genomic_DNA IPI IPI00206624; -; . PIR A23948; HHRTGB; . RefSeq NP_037215.1; NM_013083.2; . UniGene Rn.11088; -; . ProteinModelPortal P06761; -; . SMR P06761; 28-570; . IntAct P06761; 5; . MINT MINT-4575479; -; . STRING P06761; -; . PhosphoSite P06761; -; . PRIDE P06761; -; . Ensembl ENSRNOT00000025067; ENSRNOP00000025064; ENSRNOG00000018294; . GeneID 25617; -; . KEGG rno:25617; -; . NMPDR fig|10116.3.peg.18461; -; . UCSC NM_013083; rat; . CTD 3309; -; . RGD 2843; Hspa5; . eggNOG maNOG11047; -; . GeneTree ENSGT00600000084521; -; . HOVERGEN HBG051845; -; . InParanoid P06761; -; . OMA NQLTTNP; -; . OrthoDB EOG444KJV; -; . PhylomeDB P06761; -; . NextBio 607375; -; . ArrayExpress P06761; -; . Genevestigator P06761; -; . GermOnline ENSRNOG00000018294; Rattus norvegicus; . GO GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell; . GO GO:0042470; C:melanosome; IEA:UniProtKB-SubCell; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0051082; F:unfolded protein binding; IPI:RGD; . InterPro IPR018181; Heat_shock_70_CS; . InterPro IPR001023; Hsp70; . InterPro IPR013126; Hsp_70; . KO K09490; -; . PANTHER PTHR19375; Hsp70; 1; . Pfam PF00012; HSP70; 1; . PRINTS PR00301; HEATSHOCK70; . PROSITE PS00014; ER_TARGET; 1; . PROSITE PS00297; HSP70_1; 1; . PROSITE PS00329; HSP70_2; 1; . PROSITE PS01036; HSP70_3; 1; . World-2DPAGE Repository (search AC)