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Searching in 'World-2DPAGE Repository [0030]' for entry matching: GRP78_RAT




World-2DPAGE Repository (0030):  GRP78_RAT


GRP78_RAT


General information about the entry
View entry in simple text format
Entry nameGRP78_RAT
Primary accession numberP06761
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName: Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy chain-binding protein; Short=BiP; AltName: Full=Steroidogenesis-activator polypeptide; Flags: Precursor;.
Gene nameName=Hspa5
Synonyms=Grp78
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.03; Mw=82102  [identification data]
pI=4.72; Mw=81536  [identification data]
pI=4.75; Mw=81649  [identification data]
pI=4.79; Mw=81536  [identification data]
pI=4.84; Mw=81424  [identification data]
pI=4.82; Mw=81424  [identification data]
pI=4.86; Mw=81086  [identification data]
pI=4.96; Mw=81086  [identification data]
pI=4.89; Mw=80862  [identification data]
pI=5.61; Mw=49896  [identification data]
pI=4.92; Mw=80416  [identification data]

IDENTIFICATION: SPOT 499: Mascot protein score=895. Number of peptides=11 [1]
SPOT 509: Mascot protein score=56. Number of peptides=4 [1]; SPOT 510: Mascot protein score=51. Number of peptides=4 [1]; SPOT 515: Mascot protein score=85. Number of peptides=7 [1]; SPOT 516: Mascot protein score=67. Number of peptides=4 [1]; SPOT 519: Mascot protein score=312. Number of peptides=9 [1]; SPOT 525: Mascot protein score=540. Number of peptides=14 [1]; SPOT 529: Mascot protein score=72. Number of peptides=5 [1]; SPOT 531: Mascot protein score=989. Number of peptides=15 [1]; SPOT 1394: Mascot protein score=293. Number of peptides=9 [1]; SPOT 2988: Mascot protein score=652. Number of peptides=9 [1].
MAPPING (identification):
SPOT 499: Tandem mass spectrometry [1];
SPOT 509: Tandem mass spectrometry [1];
SPOT 510: Tandem mass spectrometry [1];
SPOT 515: Tandem mass spectrometry [1];
SPOT 516: Tandem mass spectrometry [1];
SPOT 519: Tandem mass spectrometry [1];
SPOT 525: Tandem mass spectrometry [1];
SPOT 529: Tandem mass spectrometry [1];
SPOT 531: Tandem mass spectrometry [1];
SPOT 1394: Tandem mass spectrometry [1];
SPOT 2988: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP06761; GRP78_RAT.
World-2DPAGE RepositoryP06761; GRP78_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameGRP78_RAT
Primary accession numberP06761
Sequence was last modified on April 1, 1988 (version 1)
Annotations were last modified on November 16, 2011 (version 117)
Name and origin of the protein
DescriptionRecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName: Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy chain-binding protein; Short=BiP; AltName: Full=Steroidogenesis-activator polypeptide; Flags: Precursor;
Gene nameName=Hspa5
Synonyms=Grp78
Encoded onName=Hspa5; Synonyms=Grp78
KeywordsAcetylation; ATP-binding; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Nucleotide-binding; Phosphoprotein; Reference proteome; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM14050; AAA40817.1; -; mRNA
EMBLBC062017; AAH62017.1; -; mRNA
EMBLM14866; AAA41277.1; -; Genomic_DNA
IPIIPI00206624; -; .
PIRA23948; HHRTGB; .
RefSeqNP_037215.1; NM_013083.2; .
UniGeneRn.11088; -; .
ProteinModelPortalP06761; -; .
SMRP06761; 28-570; .
IntActP06761; 5; .
MINTMINT-4575479; -; .
STRINGP06761; -; .
PhosphoSiteP06761; -; .
PRIDEP06761; -; .
EnsemblENSRNOT00000025067; ENSRNOP00000025064; ENSRNOG00000018294; .
GeneID25617; -; .
KEGGrno:25617; -; .
NMPDRfig|10116.3.peg.18461; -; .
UCSCNM_013083; rat; .
CTD3309; -; .
RGD2843; Hspa5; .
eggNOGmaNOG11047; -; .
GeneTreeENSGT00600000084521; -; .
HOVERGENHBG051845; -; .
InParanoidP06761; -; .
OMANQLTTNP; -; .
OrthoDBEOG444KJV; -; .
PhylomeDBP06761; -; .
NextBio607375; -; .
ArrayExpressP06761; -; .
GenevestigatorP06761; -; .
GermOnlineENSRNOG00000018294; Rattus norvegicus; .
GOGO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; IPI:RGD; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
KOK09490; -; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
PROSITEPS00014; ER_TARGET; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server