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Searching in 'World-2DPAGE Repository [0022]' for entry matching: HCDH_RAT




World-2DPAGE Repository (0022):  HCDH_RAT


HCDH_RAT


General information about the entry
View entry in simple text format
Entry nameHCDH_RAT
Primary accession numberQ9WVK7
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; Short=HCDH; EC=1.1.1.35; AltName: Full=Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; AltName: Full=Short-chain 3-hydroxyacyl-CoA dehydrogenase; Flags: Precursor;.
Gene nameName=Hadh
Synonyms=Had, Hadhsc, Schad
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=8.83; Mw=34540  [identification data]
pI=8.83; Mw=34540  [identification data]
pI=8.83; Mw=34540  [identification data]
pI=8.83; Mw=34540  [identification data]

IDENTIFICATION: SPOT 80: SeqCov=39.8%. Peptides MS=13/20. Mascot PMF Score=114 [1]
SPOT 111: SeqCov=33.1%. Peptides MS=8/20. Mascot PMF Score=69 [1]; SPOT 143: SeqCov=41.1%. Peptides MS=13/20. Mascot PMF Score=114 [1]; SPOT 209: SeqCov=24.8%. Peptides MS=7/20. Mascot PMF Score=52 [1].
PEPTIDE EVIDENCE: SPOT 80: 222-LLVPYLIEAIR-232 (68) / 137-FAAEHTIFASNTSSLQITNIANATTR-162 (96) [1]
SPOT 111: 222-LLVPYLIEAIR-232 (56) [1]; SPOT 143: 213-DTPGFIVNR-221 (54) / 222-LLVPYLIEAIR-232 (86) / 137-FAAEHTIFASNTSSLQITNIANATTR-162 (95) [1]; SPOT 209: 222-LLVPYLIEAIR-232 (42) [1].
MAPPING (identification):
SPOT 80: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 111: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 143: Peptide mass fingerprinting [1];
SPOT 209: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtQ9WVK7; HCDH_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameHCDH_RAT
Primary accession numberQ9WVK7
Sequence was last modified on November 1, 1999 (version 1)
Annotations were last modified on November 16, 2011 (version 99)
Name and origin of the protein
DescriptionRecName: Full=Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; Short=HCDH; EC=1.1.1.35; AltName: Full=Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; AltName: Full=Short-chain 3-hydroxyacyl-CoA dehydrogenase; Flags: Precursor;
Gene nameName=Hadh
Synonyms=Had, Hadhsc, Schad
Encoded onName=Hadh; Synonyms=Had, Hadhsc, Schad
KeywordsAcetylation; Complete proteome; Fatty acid metabolism; Lipid metabolism; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF095449; AAD42162.1; -; mRNA
IPIIPI00205157; -; .
PIRS74114; S74114; .
RefSeqNP_476534.1; NM_057186.1; .
UniGeneRn.92789; -; .
ProteinModelPortalQ9WVK7; -; .
SMRQ9WVK7; 24-314; .
IntActQ9WVK7; 1; .
STRINGQ9WVK7; -; .
PRIDEQ9WVK7; -; .
EnsemblENSRNOT00000014658; ENSRNOP00000014658; ENSRNOG00000010697; .
GeneID113965; -; .
KEGGrno:113965; -; .
NMPDRfig|10116.3.peg.17024; -; .
UCSCNM_057186; rat; .
CTD3033; -; .
RGD69321; Hadh; .
eggNOGroNOG15739; -; .
GeneTreeENSGT00530000063042; -; .
HOVERGENHBG000832; -; .
InParanoidQ9WVK7; -; .
OMAMGPFELS; -; .
OrthoDBEOG44BB2Z; -; .
PhylomeDBQ9WVK7; -; .
NextBio618105; -; .
ArrayExpressQ9WVK7; -; .
GenevestigatorQ9WVK7; -; .
GermOnlineENSRNOG00000010697; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IMP:RGD; .
GOGO:0070403; F:NAD+ binding; IEA:InterPro; .
GOGO:0006635; P:fatty acid beta-oxidation; IMP:RGD; .
GOGO:0046676; P:negative regulation of insulin secretion; IMP:RGD; .
GOGO:0014823; P:response to activity; IDA:RGD; .
GOGO:0042493; P:response to drug; IDA:RGD; .
GOGO:0032868; P:response to insulin stimulus; IEP:RGD; .
InterProIPR022694; 3-OHacyl-CoA_DH; .
InterProIPR006180; 3-OHacyl-CoA_DH_CS; .
InterProIPR006176; 3-OHacyl-CoA_DH_NAD-bd; .
InterProIPR006108; 3HC_DH_C; .
InterProIPR008927; 6-PGluconate_DH_C-like; .
InterProIPR013328; DH_multihelical; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
Gene3DG3DSA:1.10.1040.10; Opine_DH; 1; .
KOK00022; -; .
PfamPF00725; 3HCDH; 1; .
PfamPF02737; 3HCDH_N; 1; .
PIRSFPIRSF000105; HCDH; 1; .
SUPFAMSSF48179; 6DGDH_C_like; 1; .
PROSITEPS00067; 3HCDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server