resource logo

World-2DPAGE Repository

Attention: World-2DPAGE is no longer maintained.
It will be discontinued on 31-May-2024.

World-2DPAGE Repository no longer accepts submissions.


World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Databases

use 'Ctrl' to select several
databases


Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal

Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0027]' for entry matching: IDH3A_MOUSE




World-2DPAGE Repository (0027):  IDH3A_MOUSE


IDH3A_MOUSE


General information about the entry
View entry in simple text format
Entry nameIDH3A_MOUSE
Primary accession numberQ9D6R2
integrated into World-2DPAGE Repository (0027) on September 30, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;.
Gene nameName=Idh3a
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/prca.201000103;
Blutke A., Block C., Berendt F., Herbach N., Kemter E., Amann K., Frolich T., Arnold G.J., Wanke R.
''Differential glomerular proteome analysis of two murine nephropathy models at onset of albuminuria''
Proteomics - Clinical Applications 5(6):375-381 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

GIPRDN_4-7 {glomerular protein lysates of a GIPRdn-tg animal}
Mus musculus (Mouse)
GIPRDN_4-7
  map experimental info
 
GIPRDN_4-7

MAP LOCATIONS:
pI=5.52; Mw=38000  [identification data]

EXPRESSION:
SPOT 20: Avg ratio GIPRdn-tg vs. control=1.65 (p-value=0.0039) [1].

IDENTIFICATION: SPOT 20: SeqCov=38%. Mascot protein score=316 [1].
MAPPING (identification):
SPOT 20: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Andreas Blutke, Institute of Veterinary Pathology, University Munich, Germany
Cross-references
REPRODUCTION-2DPAGEQ9D6R2; Q9D6R2.
REPRODUCTION-2DPAGEIPI00459725; IPI00459725.
REPRODUCTION-2DPAGEIPI00128904; IPI00128904.
UCD-2DPAGEQ9D6R2; IDH3A_MOUSE.
UniProtKB/Swiss-ProtQ9D6R2; IDH3A_MOUSE.
World-2DPAGE RepositoryQ9D6R2; IDH3A_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameIDH3A_MOUSE
Primary accession numberQ9D6R2
Secondary accession number(s) Q3UAM8 Q8C8A1 Q9D1L1
Sequence was last modified on June 1, 2001 (version 1)
Annotations were last modified on November 16, 2011 (version 95)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;
Gene nameName=Idh3a
Encoded onName=Idh3a
KeywordsAcetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAK003393; BAB22760.1; -; mRNA
EMBLAK010065; BAB26679.1; -; mRNA
EMBLAK032787; BAC28021.1; -; mRNA
EMBLAK047951; BAC33199.1; -; mRNA
EMBLAK150618; BAE29708.1; -; mRNA
EMBLAK151304; BAE30286.1; -; mRNA
EMBLAK152353; BAE31145.1; -; mRNA
EMBLAK153459; BAE32011.1; -; mRNA
EMBLAK158646; BAE34596.1; -; mRNA
EMBLAK159051; BAE34785.1; -; mRNA
EMBLAK168049; BAE40031.1; -; mRNA
EMBLAK168149; BAE40114.1; -; mRNA
EMBLAK169152; BAE40931.1; -; mRNA
EMBLBC034273; AAH34273.1; -; mRNA
EMBLBC049956; AAH49956.1; -; mRNA
IPIIPI00459725; -; .
IPIIPI00608078; -; .
RefSeqNP_083849.1; NM_029573.2; .
UniGeneMm.279195; -; .
ProteinModelPortalQ9D6R2; -; .
SMRQ9D6R2; 18-362; .
IntActQ9D6R2; 5; .
STRINGQ9D6R2; -; .
PhosphoSiteQ9D6R2; -; .
REPRODUCTION-2DPAGEQ9D6R2; -; .
UCD-2DPAGEQ9D6R2; -; .
PRIDEQ9D6R2; -; .
EnsemblENSMUST00000034818; ENSMUSP00000034818; ENSMUSG00000032279; .
EnsemblENSMUST00000167866; ENSMUSP00000127526; ENSMUSG00000032279; .
GeneID67834; -; .
KEGGmmu:67834; -; .
NMPDRfig|10090.3.peg.20219; -; .
UCSCuc009prg.1; mouse; .
UCSCuc009pri.1; mouse; .
CTD3419; -; .
MGIMGI:1915084; Idh3a; .
eggNOGroNOG14850; -; .
GeneTreeENSGT00550000074918; -; .
HOGENOMHBG518924; -; .
HOVERGENHBG052080; -; .
InParanoidQ9D6R2; -; .
OMARKCREAA; -; .
OrthoDBEOG4GMTX7; -; .
PhylomeDBQ9D6R2; -; .
NextBio325657; -; .
ArrayExpressQ9D6R2; -; .
BgeeQ9D6R2; -; .
CleanExMM_IDH3A; -; .
GenevestigatorQ9D6R2; -; .
GermOnlineENSMUSG00000032279; Mus musculus; .
GOGO:0005739; C:mitochondrion; IDA:MGI; .
GOGO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IEA:EC; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR001804; Isocitrate/isopropylmalate_DH; .
InterProIPR004434; Isocitrate_DH_NAD; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
KOK00030; -; .
PANTHERPTHR11835; IDH_IMDH_dimeric; 1; .
PfamPF00180; Iso_dh; 1; .
TIGRFAMsTIGR00175; Mito_nad_idh; 1; .
PROSITEPS00470; IDH_IMDH; 1; .



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server