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Searching in 'World-2DPAGE Repository [0022]' for entry matching: IDHP_RAT




World-2DPAGE Repository (0022):  IDHP_RAT

IDHP_RAT


General information about the entry
View entry in simple text format
Entry nameIDHP_RAT
Primary accession numberP56574
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NADP], mitochondrial; Short=IDH; EC=1.1.1.42; AltName: Full=ICD-M; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase; Flags: Precursor;.
Gene nameName=Idh2
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=8.88; Mw=51391  [identification data]
pI=8.88; Mw=51391  [identification data]

IDENTIFICATION: SPOT 129: SeqCov=31%. Peptides MS=14/20. Mascot PMF Score=130 [1]
SPOT 243: SeqCov=28.3%. Peptides MS=12/20. Mascot PMF Score=102 [1].
PEPTIDE EVIDENCE: SPOT 243: 283-IWYEHR-288 (32) / 81-YFDLGLPNR-89 (39) / 160-LVPGWTKPITIGR-172 (17) [1].
MAPPING (identification):
SPOT 129: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 243: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP56574; IDHP_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameIDHP_RAT
Primary accession numberP56574
Secondary accession number(s) Q6DGF1
Sequence was last modified on December 12, 2006 (version 2)
Annotations were last modified on November 16, 2011 (version 77)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NADP], mitochondrial; Short=IDH; EC=1.1.1.42; AltName: Full=ICD-M; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase; Flags: Precursor;
Gene nameName=Idh2
Encoded onName=Idh2
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; Mitochondrion; NADP; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBC076398; AAH76398.1; -; mRNA
IPIIPI00193485; -; .
RefSeqNP_001014183.1; NM_001014161.1; .
UniGeneRn.3490; -; .
ProteinModelPortalP56574; -; .
SMRP56574; 40-452; .
IntActP56574; 1; .
MINTMINT-1794004; -; .
STRINGP56574; -; .
PhosphoSiteP56574; -; .
PRIDEP56574; -; .
EnsemblENSRNOT00000019059; ENSRNOP00000019059; ENSRNOG00000013949; .
GeneID361596; -; .
KEGGrno:361596; -; .
CTD3418; -; .
RGD1597139; Idh2; .
eggNOGroNOG09103; -; .
GeneTreeENSGT00390000012547; -; .
HOVERGENHBG006119; -; .
InParanoidP56574; -; .
OMAHKIWYEH; -; .
OrthoDBEOG42V8G4; -; .
PhylomeDBP56574; -; .
NextBio676849; -; .
ArrayExpressP56574; -; .
GenevestigatorP56574; -; .
GOGO:0004450; F:isocitrate dehydrogenase (NADP+) activity; ISS:UniProtKB; .
GOGO:0000287; F:magnesium ion binding; ISS:UniProtKB; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB; .
GOGO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW; .
GOGO:0006102; P:isocitrate metabolic process; ISS:UniProtKB; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR004790; Isocitrate_DH_NADP_euk-typ; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
KOK00031; -; .
PANTHERPTHR11822; IDH_NADP_euk; 1; .
PfamPF00180; Iso_dh; 1; .
PIRSFPIRSF000108; IDH_NADP; 1; .
TIGRFAMsTIGR00127; Nadp_idh_euk; 1; .
PROSITEPS00470; IDH_IMDH; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server