World-2DPAGE Repository
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World-2DPAGE Repository
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Select Remote Interfaces
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SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
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Searching in 'World-2DPAGE Repository [0022]' for entry
matching:
IDHP_RAT
World-2DPAGE Repository
(0022):
IDHP_RAT
IDHP_RAT
General information about the entry
View entry in simple text format
Entry name
IDHP_RAT
Primary accession number
P56574
integrated into World-2DPAGE Repository (0022) on
May 7, 2010 (release 1)
2D Annotations were last modified on
June 22, 2011 (version 2)
General Annotations were last modified on
November 24, 2011 (version 2)
Name and origin of the protein
Description
RecName: Full=Isocitrate dehydrogenase [NADP], mitochondrial; Short=IDH; EC=1.1.1.42; AltName: Full=ICD-M; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase; Flags: Precursor;.
Gene name
Name=Idh2
Annotated species
Rattus [TaxID:
10114
]
Taxonomy
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]
2D-PAGE GEL CHARACTERIZATION
DOI=
10.1002/pmic.201000593
;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein
HCR_LCR_HEART
{HCR LCR Cardiac Map}
Rattus
Tissue: Heart
map experimental info
HCR_LCR_HEART
MAP LOCATIONS:
SPOT 129
:
pI=8.88; Mw=51391
[identification data]
SPOT 243
:
pI=8.88; Mw=51391
[identification data]
IDENTIFICATION:
SPOT 129: SeqCov=31%. Peptides MS=14/20. Mascot PMF Score=130 [
1
]
SPOT 243: SeqCov=28.3%. Peptides MS=12/20. Mascot PMF Score=102 [
1
].
PEPTIDE EVIDENCE:
SPOT 243: 283-IWYEHR-288 (32) / 81-YFDLGLPNR-89 (39) / 160-LVPGWTKPITIGR-172 (17) [
1
].
MAPPING (identification):
SPOT
129
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
];
SPOT
243
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
].
Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-Prot
P56574; IDHP_RAT.
2D PAGE maps for identified proteins
How to interpret a protein map
You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
Warning 1
: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
Warning 2
: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
External data extracted from
UniProtKB/Swiss-Prot
Extracted from
UniProtKB/Swiss-Prot
, release:
2011_11
Entry name
IDHP_RAT
Primary accession number
P56574
Secondary accession number(s)
Q6DGF1
Sequence was last modified on
December 12, 2006 (version 2)
Annotations were last modified on
November 16, 2011 (version 77)
Name and origin of the protein
Description
RecName: Full=Isocitrate dehydrogenase [NADP], mitochondrial; Short=IDH; EC=1.1.1.42; AltName: Full=ICD-M; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase; Flags: Precursor;
Gene name
Name=Idh2
Encoded on
Name=Idh2
Keywords
Acetylation
;
Complete proteome
;
Direct protein sequencing
;
Glyoxylate bypass
;
Magnesium
;
Manganese
;
Metal-binding
;
Mitochondrion
;
NADP
;
Oxidoreductase
;
Reference proteome
;
Transit peptide
;
Tricarboxylic acid cycle
.
Copyright
Copyrighted by the UniProt Consortium, see
https://www.uniprot.org/help/license
. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL
BC076398; AAH76398.1
; -; mRNA
IPI
IPI00193485; -
; .
RefSeq
NP_001014183.1; NM_001014161.1
; .
UniGene
Rn.3490; -
; .
ProteinModelPortal
P56574; -
; .
SMR
P56574; 40-452
; .
IntAct
P56574; 1
; .
MINT
MINT-1794004; -
; .
STRING
P56574; -
; .
PhosphoSite
P56574; -
; .
PRIDE
P56574; -
; .
Ensembl
ENSRNOT00000019059; ENSRNOP00000019059
; ENSRNOG00000013949; .
GeneID
361596; -
; .
KEGG
rno:361596; -
; .
CTD
3418; -
; .
RGD
1597139; Idh2
; .
eggNOG
roNOG09103; -
; .
GeneTree
ENSGT00390000012547; -
; .
HOVERGEN
HBG006119; -
; .
InParanoid
P56574; -
; .
OMA
HKIWYEH; -
; .
OrthoDB
EOG42V8G4; -
; .
PhylomeDB
P56574; -
; .
NextBio
676849; -
; .
ArrayExpress
P56574; -
; .
Genevestigator
P56574; -
; .
GO
GO:0004450; F:isocitrate dehydrogenase (NADP+) activity
; ISS:UniProtKB; .
GO
GO:0000287; F:magnesium ion binding
; ISS:UniProtKB; .
GO
GO:0051287; F:NAD binding
; IEA:InterPro; .
GO
GO:0006103; P:2-oxoglutarate metabolic process
; ISS:UniProtKB; .
GO
GO:0006097; P:glyoxylate cycle
; IEA:UniProtKB-KW; .
GO
GO:0006102; P:isocitrate metabolic process
; ISS:UniProtKB; .
GO
GO:0006099; P:tricarboxylic acid cycle
; IEA:UniProtKB-KW; .
InterPro
IPR019818; IsoCit/isopropylmalate_DH_CS
; .
InterPro
IPR004790; Isocitrate_DH_NADP_euk-typ
; .
InterPro
IPR024084; IsoPropMal-DH-like_dom
; .
Gene3D
G3DSA:3.40.718.10; IDH_IMDH
; 1; .
KO
K00031; -
; .
PANTHER
PTHR11822; IDH_NADP_euk
; 1; .
Pfam
PF00180; Iso_dh
; 1; .
PIRSF
PIRSF000108; IDH_NADP
; 1; .
TIGRFAMs
TIGR00127; Nadp_idh_euk
; 1; .
PROSITE
PS00470; IDH_IMDH
; 1; .
World-2DPAGE Repository (search AC)
Database constructed and maintained by
SIB
, using the
Make2D-DB II
package (
ver. 3.10.2
) from the
World-2DPAGE Constellation
of the
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