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Searching in 'World-2DPAGE Repository [0004]' for entry matching: KPYM_RAT




World-2DPAGE Repository (0004):  KPYM_RAT


KPYM_RAT


General information about the entry
View entry in simple text format
Entry nameKPYM_RAT
Primary accession numberP11980
integrated into World-2DPAGE Repository (0004) on October 13, 2008 (release 1)
2D Annotations were last modified onMay 18, 2011 (version 2)
General Annotations were last modified on December 22, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;.
Gene nameName=Pkm2
Synonyms=Pykm
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   MAPPING ON GEL
PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan]
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of the mature rat olfactory bulb''
Proteomics 9(1):2593-2599 (2009)
2D PAGE maps for identified proteins
How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb
OB21DSUB
  map experimental info
 
OB21DSUB

MAP LOCATIONS:
pI=7.10; Mw=57000  [identification data]
pI=6.89; Mw=57000  [identification data]
pI=6.90; Mw=55000  [identification data]

MAPPING (identification):
SPOT 285: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 286: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 289: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
UniProtKB/Swiss-ProtP11980; KPYM_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameKPYM_RAT
Primary accession numberP11980
Secondary accession number(s) P11981
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 113)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;
Gene nameName=Pkm2
Synonyms=Pykm
Encoded onName=Pkm2; Synonyms=Pykm
KeywordsAcetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM24359; AAB93666.1; -; Genomic_DNA
EMBLM24359; AAB93667.1; -; Genomic_DNA
EMBLM14377; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLX15800; CAA33799.1; -; mRNA
IPIIPI00231929; -; .
IPIIPI00339197; -; .
PIRA26186; A26186; .
PIRB26186; B26186; .
RefSeqNP_445749.1; NM_053297.1; .
UniGeneRn.1556; -; .
ProteinModelPortalP11980; -; .
SMRP11980; 11-531; .
IntActP11980; 1; .
MINTMINT-4587504; -; .
STRINGP11980; -; .
PhosphoSiteP11980; -; .
World-2DPAGE0004:P11980; -; .
PRIDEP11980; -; .
EnsemblENSRNOT00000015332; ENSRNOP00000015331; ENSRNOG00000011329; .
EnsemblENSRNOT00000015398; ENSRNOP00000015398; ENSRNOG00000011329; .
GeneID25630; -; .
KEGGrno:25630; -; .
UCSCNM_053297; rat; .
CTD5315; -; .
RGD3337; Pkm2; .
eggNOGmaNOG12287; -; .
GeneTreeENSGT00390000008859; -; .
HOVERGENHBG000941; -; .
InParanoidP11980; -; .
OrthoDBEOG40GCQJ; -; .
PhylomeDBP11980; -; .
NextBio607431; -; .
GenevestigatorP11980; -; .
GermOnlineENSRNOG00000011329; Rattus norvegicus; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0043531; F:ADP binding; IDA:RGD; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0030955; F:potassium ion binding; IEA:InterPro; .
GOGO:0005515; F:protein binding; IPI:RGD; .
GOGO:0004743; F:pyruvate kinase activity; IDA:RGD; .
GOGO:0006754; P:ATP biosynthetic process; IDA:RGD; .
GOGO:0006096; P:glycolysis; IDA:RGD; .
GOGO:0001889; P:liver development; IDA:RGD; .
GOGO:0031100; P:organ regeneration; IDA:RGD; .
GOGO:0042866; P:pyruvate biosynthetic process; IDA:RGD; .
GOGO:0009629; P:response to gravity; IEP:RGD; .
GOGO:0001666; P:response to hypoxia; IEP:RGD; .
GOGO:0032868; P:response to insulin stimulus; IEP:RGD; .
GOGO:0014870; P:response to muscle inactivity; IEP:RGD; .
GOGO:0007584; P:response to nutrient; IEP:RGD; .
GOGO:0043403; P:skeletal muscle tissue regeneration; IDA:RGD; .
InterProIPR001697; Pyr_Knase; .
InterProIPR015813; Pyrv/PenolPyrv_Kinase; .
InterProIPR011037; Pyrv_Knase-like_insert_dom; .
InterProIPR015794; Pyrv_Knase_a/b; .
InterProIPR018209; Pyrv_Knase_AS; .
InterProIPR015793; Pyrv_Knase_brl; .
InterProIPR015795; Pyrv_Knase_C; .
InterProIPR015806; Pyrv_Knase_insert_dom; .
Gene3DG3DSA:2.40.33.10; PK_B_barrel_like; 1; .
Gene3DG3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; .
Gene3DG3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; .
KOK00873; -; .
PANTHERPTHR11817; Pyruvate_kinase; 1; .
PfamPF00224; PK; 1; .
PfamPF02887; PK_C; 1; .
PRINTSPR01050; PYRUVTKNASE; .
SUPFAMSSF50800; PK_B_barrel_like; 1; .
SUPFAMSSF52935; Pyruvate_kinase; 1; .
SUPFAMSSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMsTIGR01064; Pyruv_kin; 1; .
PROSITEPS00110; PYRUVATE_KINASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server