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Searching in 'World-2DPAGE Repository [0030]' for entry matching: KPYR_RAT World-2DPAGE Repository (0030):  KPYR_RAT KPYR_RAT General information about the entry View entry in simple text format Entry name KPYR_RAT Primary accession number P12928 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Pyruvate kinase isozymes R/L; EC=2.7.1.40; AltName: Full=L-PK;. Gene name Name=Pklr Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 855: pI=6.55; Mw=67180   [identification data] SPOT 891: pI=6.54; Mw=66257   [identification data] SPOT 1051: pI=5.92; Mw=61064   [identification data] IDENTIFICATION: SPOT 855: Mascot protein score=112. Number of peptides=7 [1] SPOT 891: Mascot protein score=36. Number of peptides=2 [1]; SPOT 1051: Mascot protein score=62. Number of peptides=4 [1]. MAPPING (identification): SPOT 855: Tandem mass spectrometry [1]; SPOT 891: Tandem mass spectrometry [1]; SPOT 1051: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot P12928; KPYR_RAT. World-2DPAGE Repository P12928; KPYR_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name KPYR_RAT Primary accession number P12928 Secondary accession number(s) P04763 Q64618 Sequence was last modified on  February 1, 1995 (version 2) Annotations were last modified on  November 16, 2011 (version 116) Name and origin of the protein Description RecName: Full=Pyruvate kinase isozymes R/L; EC=2.7.1.40; AltName: Full=L-PK; Gene name Name=Pklr Encoded on Name=Pklr Keywords Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M17091; AAA41882.1; -; Genomic_DNA EMBL M17088; AAA41882.1; JOINED; Genomic_DNA EMBL M17089; AAA41882.1; JOINED; Genomic_DNA EMBL M17090; AAA41882.1; JOINED; Genomic_DNA EMBL M17091; AAA41883.1; -; Genomic_DNA EMBL M17088; AAA41883.1; JOINED; Genomic_DNA EMBL M17089; AAA41883.1; JOINED; Genomic_DNA EMBL M17090; AAA41883.1; JOINED; Genomic_DNA EMBL M17685; AAA41881.1; -; mRNA EMBL X05684; CAA29169.1; -; Genomic_DNA EMBL M11709; AAA41880.1; -; mRNA IPI IPI00202549; -; . IPI IPI00231683; -; . PIR A27427; KIRTPR; . PIR A92940; KIRTPL; . RefSeq NP_036756.3; NM_012624.3; . UniGene Rn.48821; -; . ProteinModelPortal P12928; -; . SMR P12928; 57-573; . STRING P12928; -; . PhosphoSite P12928; -; . PRIDE P12928; -; . Ensembl ENSRNOT00000027700; ENSRNOP00000027700; ENSRNOG00000020420; . Ensembl ENSRNOT00000065791; ENSRNOP00000058886; ENSRNOG00000020420; . GeneID 24651; -; . KEGG rno:24651; -; . NMPDR fig|10116.3.peg.16404; -; . UCSC NM_012624; rat; . CTD 5313; -; . RGD 3336; Pklr; . eggNOG maNOG16019; -; . GeneTree ENSGT00390000008859; -; . HOVERGEN HBG000941; -; . InParanoid P12928; -; . OMA VKGSQVL; -; . OrthoDB EOG40GCQJ; -; . PhylomeDB P12928; -; . ArrayExpress P12928; -; . Genevestigator P12928; -; . GermOnline ENSRNOG00000020420; Rattus norvegicus; . GO GO:0005829; C:cytosol; IDA:RGD; . GO GO:0005625; C:soluble fraction; IDA:RGD; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0000287; F:magnesium ion binding; IEA:InterPro; . GO GO:0030955; F:potassium ion binding; IEA:InterPro; . GO GO:0004743; F:pyruvate kinase activity; IDA:RGD; . GO GO:0006754; P:ATP biosynthetic process; IDA:RGD; . GO GO:0032869; P:cellular response to insulin stimulus; IDA:RGD; . GO GO:0006096; P:glycolysis; IDA:RGD; . GO GO:0042866; P:pyruvate biosynthetic process; IDA:RGD; . GO GO:0033198; P:response to ATP; IDA:RGD; . GO GO:0051591; P:response to cAMP; IDA:RGD; . GO GO:0009749; P:response to glucose stimulus; IEP:RGD; . GO GO:0009408; P:response to heat; IDA:RGD; . GO GO:0001666; P:response to hypoxia; IEP:RGD; . GO GO:0010226; P:response to lithium ion; IDA:RGD; . GO GO:0007584; P:response to nutrient; IEP:RGD; . GO GO:0014070; P:response to organic cyclic compound; IEP:RGD; . InterPro IPR001697; Pyr_Knase; . InterPro IPR015813; Pyrv/PenolPyrv_Kinase; . InterPro IPR011037; Pyrv_Knase-like_insert_dom; . InterPro IPR015794; Pyrv_Knase_a/b; . InterPro IPR018209; Pyrv_Knase_AS; . InterPro IPR015793; Pyrv_Knase_brl; . InterPro IPR015795; Pyrv_Knase_C; . InterPro IPR015806; Pyrv_Knase_insert_dom; . Gene3D G3DSA:2.40.33.10; PK_B_barrel_like; 1; . Gene3D G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; . Gene3D G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; . KO K12406; -; . PANTHER PTHR11817; Pyruvate_kinase; 1; . Pfam PF00224; PK; 1; . Pfam PF02887; PK_C; 1; . PRINTS PR01050; PYRUVTKNASE; . SUPFAM SSF50800; PK_B_barrel_like; 1; . SUPFAM SSF52935; Pyruvate_kinase; 1; . SUPFAM SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; . TIGRFAMs TIGR01064; Pyruv_kin; 1; . PROSITE PS00110; PYRUVATE_KINASE; 1; . World-2DPAGE Repository (search AC)