Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 527:

pI=5.77; Mw=37000

SPOT 528:

pI=5.92; Mw=37000

MAPPING (identification):

SPOT 527: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 528: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	MDHC_RAT
Primary accession number	O88989
Secondary accession number(s)	O88585 Q6PCV2
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 84)
Name and origin of the protein
Description	RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene name	Name=Mdh1 Synonyms=Mdh
Encoded on	Name=Mdh1; Synonyms=Mdh
Keywords	Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AF093773; AAC64180.1; -; mRNA
EMBL	BC059124; AAH59124.1; -; mRNA
EMBL	AF075574; AAC26799.1; -; mRNA
IPI	IPI00198717; -; .
RefSeq	NP_150238.1; NM_033235.1; .
UniGene	Rn.13492; -; .
ProteinModelPortal	O88989; -; .
SMR	O88989; 2-334; .
IntAct	O88989; 1; .
STRING	O88989; -; .
PhosphoSite	O88989; -; .
World-2DPAGE	0004:O88989; -; .
PRIDE	O88989; -; .
Ensembl	ENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID	24551; -; .
KEGG	rno:24551; -; .
NMPDR	fig\|10116.3.peg.10844; -; .
UCSC	NM_033235; rat; .
CTD	4190; -; .
RGD	3072; Mdh1; .
eggNOG	roNOG11216; -; .
GeneTree	ENSGT00530000063410; -; .
HOVERGEN	HBG006340; -; .
InParanoid	O88989; -; .
OrthoDB	EOG4CVG78; -; .
PhylomeDB	O88989; -; .
NextBio	603654; -; .
ArrayExpress	O88989; -; .
Genevestigator	O88989; -; .
GermOnline	ENSRNOG00000008103; Rattus norvegicus; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GO	GO:0051287; F:NAD binding; IDA:RGD; .
GO	GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GO	GO:0006108; P:malate metabolic process; IDA:RGD; .
GO	GO:0006734; P:NADH metabolic process; IDA:RGD; .
GO	GO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR001557; L-lactate/malate_DH; .
InterPro	IPR022383; Lactate/malate_DH_C; .
InterPro	IPR001236; Lactate/malate_DH_N; .
InterPro	IPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterPro	IPR001252; Malate_DH_AS; .
InterPro	IPR011274; Malate_DH_NAD-dep_euk; .
InterPro	IPR010945; Malate_DH_type2; .
InterPro	IPR016040; NAD(P)-bd_dom; .
Gene3D	G3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3D	G3DSA:3.40.50.720; NAD(P)-bd; 1; .
KO	K00025; -; .
PANTHER	PTHR23382; MDH_SF1; 1; .
Pfam	PF02866; Ldh_1_C; 1; .
Pfam	PF00056; Ldh_1_N; 1; .
PIRSF	PIRSF000102; Lac_mal_DH; 1; .
SUPFAM	SSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMs	TIGR01759; MalateDH-SF1; 1; .
TIGRFAMs	TIGR01758; MDH_euk_cyt; 1; .
PROSITE	PS00068; MDH; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: MDHC_RAT

World-2DPAGE Repository (0014): MDHC_RAT

MDHC_RAT

General information about the entry

Entry name

MDHC_RAT

Primary accession number

O88989

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.

Gene name

Name=Mdh1
Synonyms=Mdh

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 3028:

pI=6.75; Mw=38000

IDENTIFICATION: SPOT 3028: SeqCov=38%. MascotPMF=73 [1].
MAPPING (identification):

SPOT 3028: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	MDHC_RAT
Primary accession number	O88989
Secondary accession number(s)	O88585 Q6PCV2
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 84)
Name and origin of the protein
Description	RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene name	Name=Mdh1 Synonyms=Mdh
Encoded on	Name=Mdh1; Synonyms=Mdh
Keywords	Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AF093773; AAC64180.1; -; mRNA
EMBL	BC059124; AAH59124.1; -; mRNA
EMBL	AF075574; AAC26799.1; -; mRNA
IPI	IPI00198717; -; .
RefSeq	NP_150238.1; NM_033235.1; .
UniGene	Rn.13492; -; .
ProteinModelPortal	O88989; -; .
SMR	O88989; 2-334; .
IntAct	O88989; 1; .
STRING	O88989; -; .
PhosphoSite	O88989; -; .
World-2DPAGE	0004:O88989; -; .
PRIDE	O88989; -; .
Ensembl	ENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID	24551; -; .
KEGG	rno:24551; -; .
NMPDR	fig\|10116.3.peg.10844; -; .
UCSC	NM_033235; rat; .
CTD	4190; -; .
RGD	3072; Mdh1; .
eggNOG	roNOG11216; -; .
GeneTree	ENSGT00530000063410; -; .
HOVERGEN	HBG006340; -; .
InParanoid	O88989; -; .
OrthoDB	EOG4CVG78; -; .
PhylomeDB	O88989; -; .
NextBio	603654; -; .
ArrayExpress	O88989; -; .
Genevestigator	O88989; -; .
GermOnline	ENSRNOG00000008103; Rattus norvegicus; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GO	GO:0051287; F:NAD binding; IDA:RGD; .
GO	GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GO	GO:0006108; P:malate metabolic process; IDA:RGD; .
GO	GO:0006734; P:NADH metabolic process; IDA:RGD; .
GO	GO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR001557; L-lactate/malate_DH; .
InterPro	IPR022383; Lactate/malate_DH_C; .
InterPro	IPR001236; Lactate/malate_DH_N; .
InterPro	IPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterPro	IPR001252; Malate_DH_AS; .
InterPro	IPR011274; Malate_DH_NAD-dep_euk; .
InterPro	IPR010945; Malate_DH_type2; .
InterPro	IPR016040; NAD(P)-bd_dom; .
Gene3D	G3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3D	G3DSA:3.40.50.720; NAD(P)-bd; 1; .
KO	K00025; -; .
PANTHER	PTHR23382; MDH_SF1; 1; .
Pfam	PF02866; Ldh_1_C; 1; .
Pfam	PF00056; Ldh_1_N; 1; .
PIRSF	PIRSF000102; Lac_mal_DH; 1; .
SUPFAM	SSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMs	TIGR01759; MalateDH-SF1; 1; .
TIGRFAMs	TIGR01758; MDH_euk_cyt; 1; .
PROSITE	PS00068; MDH; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: MDHC_RAT

World-2DPAGE Repository (0019): MDHC_RAT

MDHC_RAT

General information about the entry

Entry name

MDHC_RAT

Primary accession number

O88989

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.

Gene name

Name=Mdh1
Synonyms=Mdh

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 648:

pI=5.93; Mw=36632

SPOT 653:

pI=5.93; Mw=36632

SPOT 1096:

pI=5.93; Mw=36632

IDENTIFICATION: SPOT 648: Peptides number=6. SeqCov=17.7%. MOWSE score=5.71E+4 [1]
SPOT 653: Peptides number=8. SeqCov=30.2%. MOWSE score=4.54E+8 [1]; SPOT 1096: Peptides number=7. SeqCov=22.5%. MOWSE score=9.70E+6 [1].
MAPPING (identification):

SPOT 648: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 653: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1096: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	MDHC_RAT
Primary accession number	O88989
Secondary accession number(s)	O88585 Q6PCV2
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 84)
Name and origin of the protein
Description	RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene name	Name=Mdh1 Synonyms=Mdh
Encoded on	Name=Mdh1; Synonyms=Mdh
Keywords	Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AF093773; AAC64180.1; -; mRNA
EMBL	BC059124; AAH59124.1; -; mRNA
EMBL	AF075574; AAC26799.1; -; mRNA
IPI	IPI00198717; -; .
RefSeq	NP_150238.1; NM_033235.1; .
UniGene	Rn.13492; -; .
ProteinModelPortal	O88989; -; .
SMR	O88989; 2-334; .
IntAct	O88989; 1; .
STRING	O88989; -; .
PhosphoSite	O88989; -; .
World-2DPAGE	0004:O88989; -; .
PRIDE	O88989; -; .
Ensembl	ENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID	24551; -; .
KEGG	rno:24551; -; .
NMPDR	fig\|10116.3.peg.10844; -; .
UCSC	NM_033235; rat; .
CTD	4190; -; .
RGD	3072; Mdh1; .
eggNOG	roNOG11216; -; .
GeneTree	ENSGT00530000063410; -; .
HOVERGEN	HBG006340; -; .
InParanoid	O88989; -; .
OrthoDB	EOG4CVG78; -; .
PhylomeDB	O88989; -; .
NextBio	603654; -; .
ArrayExpress	O88989; -; .
Genevestigator	O88989; -; .
GermOnline	ENSRNOG00000008103; Rattus norvegicus; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GO	GO:0051287; F:NAD binding; IDA:RGD; .
GO	GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GO	GO:0006108; P:malate metabolic process; IDA:RGD; .
GO	GO:0006734; P:NADH metabolic process; IDA:RGD; .
GO	GO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR001557; L-lactate/malate_DH; .
InterPro	IPR022383; Lactate/malate_DH_C; .
InterPro	IPR001236; Lactate/malate_DH_N; .
InterPro	IPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterPro	IPR001252; Malate_DH_AS; .
InterPro	IPR011274; Malate_DH_NAD-dep_euk; .
InterPro	IPR010945; Malate_DH_type2; .
InterPro	IPR016040; NAD(P)-bd_dom; .
Gene3D	G3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3D	G3DSA:3.40.50.720; NAD(P)-bd; 1; .
KO	K00025; -; .
PANTHER	PTHR23382; MDH_SF1; 1; .
Pfam	PF02866; Ldh_1_C; 1; .
Pfam	PF00056; Ldh_1_N; 1; .
PIRSF	PIRSF000102; Lac_mal_DH; 1; .
SUPFAM	SSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMs	TIGR01759; MalateDH-SF1; 1; .
TIGRFAMs	TIGR01758; MDH_euk_cyt; 1; .
PROSITE	PS00068; MDH; 1; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: MDHC_RAT

World-2DPAGE Repository (0022): MDHC_RAT

MDHC_RAT

General information about the entry

Entry name

MDHC_RAT

Primary accession number

O88989

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.

Gene name

Name=Mdh1
Synonyms=Mdh

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 348:

pI=6.16; Mw=36631

SPOT 440:

pI=6.16; Mw=36631

SPOT 513:

pI=6.16; Mw=36631

SPOT 514:

pI=6.16; Mw=36631

IDENTIFICATION: SPOT 348: SeqCov=37.7%. Peptides MS=12/20. Mascot PMF Score=103 [1]
SPOT 440: SeqCov=27.2%. Peptides MS=9/20. Mascot PMF Score=63 [1]; SPOT 513: SeqCov=32%. Peptides MS=10/20. Mascot PMF Score=77 [1]; SPOT 514: SeqCov=40.1%. Peptides MS=14/20. Mascot PMF Score=122 [1].
PEPTIDE EVIDENCE: SPOT 348: 221-GEFITTVQQR-230 (68) / 299-FVEGLPINDFSR-310 (77) / 180-NVIIWGNHSSTQYPDVNHAK-199 (95) [1]
SPOT 440: 221-GEFITTVQQR-230 (62) / 299-FVEGLPINDFSR-310 (88) [1]; SPOT 513: 221-GEFITTVQQR-230 (49) / 299-FVEGLPINDFSR-310 (69) [1].
MAPPING (identification):

SPOT 348: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 440: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 513: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 514: Peptide mass fingerprinting [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	MDHC_RAT
Primary accession number	O88989
Secondary accession number(s)	O88585 Q6PCV2
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 84)
Name and origin of the protein
Description	RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene name	Name=Mdh1 Synonyms=Mdh
Encoded on	Name=Mdh1; Synonyms=Mdh
Keywords	Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AF093773; AAC64180.1; -; mRNA
EMBL	BC059124; AAH59124.1; -; mRNA
EMBL	AF075574; AAC26799.1; -; mRNA
IPI	IPI00198717; -; .
RefSeq	NP_150238.1; NM_033235.1; .
UniGene	Rn.13492; -; .
ProteinModelPortal	O88989; -; .
SMR	O88989; 2-334; .
IntAct	O88989; 1; .
STRING	O88989; -; .
PhosphoSite	O88989; -; .
World-2DPAGE	0004:O88989; -; .
PRIDE	O88989; -; .
Ensembl	ENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID	24551; -; .
KEGG	rno:24551; -; .
NMPDR	fig\|10116.3.peg.10844; -; .
UCSC	NM_033235; rat; .
CTD	4190; -; .
RGD	3072; Mdh1; .
eggNOG	roNOG11216; -; .
GeneTree	ENSGT00530000063410; -; .
HOVERGEN	HBG006340; -; .
InParanoid	O88989; -; .
OrthoDB	EOG4CVG78; -; .
PhylomeDB	O88989; -; .
NextBio	603654; -; .
ArrayExpress	O88989; -; .
Genevestigator	O88989; -; .
GermOnline	ENSRNOG00000008103; Rattus norvegicus; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GO	GO:0051287; F:NAD binding; IDA:RGD; .
GO	GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GO	GO:0006108; P:malate metabolic process; IDA:RGD; .
GO	GO:0006734; P:NADH metabolic process; IDA:RGD; .
GO	GO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR001557; L-lactate/malate_DH; .
InterPro	IPR022383; Lactate/malate_DH_C; .
InterPro	IPR001236; Lactate/malate_DH_N; .
InterPro	IPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterPro	IPR001252; Malate_DH_AS; .
InterPro	IPR011274; Malate_DH_NAD-dep_euk; .
InterPro	IPR010945; Malate_DH_type2; .
InterPro	IPR016040; NAD(P)-bd_dom; .
Gene3D	G3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3D	G3DSA:3.40.50.720; NAD(P)-bd; 1; .
KO	K00025; -; .
PANTHER	PTHR23382; MDH_SF1; 1; .
Pfam	PF02866; Ldh_1_C; 1; .
Pfam	PF00056; Ldh_1_N; 1; .
PIRSF	PIRSF000102; Lac_mal_DH; 1; .
SUPFAM	SSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMs	TIGR01759; MalateDH-SF1; 1; .
TIGRFAMs	TIGR01758; MDH_euk_cyt; 1; .
PROSITE	PS00068; MDH; 1; .

Searching in 'World-2DPAGE Repository [0030]' for entry matching: MDHC_RAT

World-2DPAGE Repository (0030): MDHC_RAT

MDHC_RAT

General information about the entry

Entry name

MDHC_RAT

Primary accession number

O88989

integrated into World-2DPAGE Repository (0030) on

November 9, 2010 (release 1)

2D Annotations were last modified on

June 21, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.

Gene name

Name=Mdh1
Synonyms=Mdh

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011)

2D PAGE maps for identified proteins

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)

RAT_INS1E_4-7

MAP LOCATIONS:

SPOT 1608:

pI=6.19; Mw=42735

SPOT 1678:

pI=6.21; Mw=40884

SPOT 1702:

pI=6.17; Mw=40044

IDENTIFICATION: SPOT 1608: Mascot protein score=45. Number of peptides=2 [1]
SPOT 1678: Mascot protein score=82. Number of peptides=2 [1]; SPOT 1702: Mascot protein score=170. Number of peptides=4 [1].
MAPPING (identification):

SPOT 1608: Tandem mass spectrometry [1];
SPOT 1678: Tandem mass spectrometry [1];
SPOT 1702: Tandem mass spectrometry [1].

Copyright

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium

Cross-references

UniProtKB/Swiss-Prot

World-2DPAGE Repository

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	MDHC_RAT
Primary accession number	O88989
Secondary accession number(s)	O88585 Q6PCV2
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 84)
Name and origin of the protein
Description	RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene name	Name=Mdh1 Synonyms=Mdh
Encoded on	Name=Mdh1; Synonyms=Mdh
Keywords	Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AF093773; AAC64180.1; -; mRNA
EMBL	BC059124; AAH59124.1; -; mRNA
EMBL	AF075574; AAC26799.1; -; mRNA
IPI	IPI00198717; -; .
RefSeq	NP_150238.1; NM_033235.1; .
UniGene	Rn.13492; -; .
ProteinModelPortal	O88989; -; .
SMR	O88989; 2-334; .
IntAct	O88989; 1; .
STRING	O88989; -; .
PhosphoSite	O88989; -; .
World-2DPAGE	0004:O88989; -; .
PRIDE	O88989; -; .
Ensembl	ENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID	24551; -; .
KEGG	rno:24551; -; .
NMPDR	fig\|10116.3.peg.10844; -; .
UCSC	NM_033235; rat; .
CTD	4190; -; .
RGD	3072; Mdh1; .
eggNOG	roNOG11216; -; .
GeneTree	ENSGT00530000063410; -; .
HOVERGEN	HBG006340; -; .
InParanoid	O88989; -; .
OrthoDB	EOG4CVG78; -; .
PhylomeDB	O88989; -; .
NextBio	603654; -; .
ArrayExpress	O88989; -; .
Genevestigator	O88989; -; .
GermOnline	ENSRNOG00000008103; Rattus norvegicus; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GO	GO:0051287; F:NAD binding; IDA:RGD; .
GO	GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GO	GO:0006108; P:malate metabolic process; IDA:RGD; .
GO	GO:0006734; P:NADH metabolic process; IDA:RGD; .
GO	GO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR001557; L-lactate/malate_DH; .
InterPro	IPR022383; Lactate/malate_DH_C; .
InterPro	IPR001236; Lactate/malate_DH_N; .
InterPro	IPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterPro	IPR001252; Malate_DH_AS; .
InterPro	IPR011274; Malate_DH_NAD-dep_euk; .
InterPro	IPR010945; Malate_DH_type2; .
InterPro	IPR016040; NAD(P)-bd_dom; .
Gene3D	G3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3D	G3DSA:3.40.50.720; NAD(P)-bd; 1; .
KO	K00025; -; .
PANTHER	PTHR23382; MDH_SF1; 1; .
Pfam	PF02866; Ldh_1_C; 1; .
Pfam	PF00056; Ldh_1_N; 1; .
PIRSF	PIRSF000102; Lac_mal_DH; 1; .
SUPFAM	SSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMs	TIGR01759; MalateDH-SF1; 1; .
TIGRFAMs	TIGR01758; MDH_euk_cyt; 1; .
PROSITE	PS00068; MDH; 1; .

Searching in 'World-2DPAGE Repository [0069]' for entry matching: MDHC_RAT

World-2DPAGE Repository (0069): MDHC_RAT

MDHC_RAT

General information about the entry

Entry name

MDHC_RAT

Primary accession number

MDHC_RAT

integrated into World-2DPAGE Repository (0069) on

February 27, 2014 (release 1)

2D Annotations were last modified on

February 27, 2014 (version 1)

General Annotations were last modified on

February 27, 2014 (version 1)

Name and origin of the protein

Description

MDHC_RAT.

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D GEL CHARACTERIZATION
DOI=10.1016/j.jprot.2014.04.015;
Burniston J.G., Kenyani J., Gray D., Guadagnin E., Jarman I.H., Cobley J.N., Cuthbertson D.J., Chen Y.-W., Wastling J.M., Lisboa P., Koch L.G., Britton S.L.
''Conditional independence mapping of DIGE data reveals PDIA3 protein species as key nodes associated with muscle aerobic capacity''
Journal of Proteomics 106(1):230-245 (2014)

2D PAGE maps for identified proteins

RATTUS_SOLEUS-DIGE_4.5-9.5 {M+F HCR LCR Soleus DiGE Exp}
Rattus
Tissue: Skeletal muscle