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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0022]' for entry matching: MDHC_RAT World-2DPAGE Repository (0022):  MDHC_RAT MDHC_RAT General information about the entry View entry in simple text format Entry name MDHC_RAT Primary accession number O88989 integrated into World-2DPAGE Repository (0022) on  May 7, 2010 (release 1) 2D Annotations were last modified on June 22, 2011 (version 2) General Annotations were last modified on  November 24, 2011 (version 2) Name and origin of the protein Description RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;. Gene name Name=Mdh1 Synonyms=Mdh Annotated species Rattus [TaxID: 10114] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000593; Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L. ''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity'' Proteomics 11(16):3369-3379 (2011) 2D PAGE maps for identified proteins How to interpret a protein HCR_LCR_HEART {HCR LCR Cardiac Map} Rattus Tissue: Heart   map experimental info   HCR_LCR_HEART MAP LOCATIONS: SPOT 348: pI=6.16; Mw=36631   [identification data] SPOT 440: pI=6.16; Mw=36631   [identification data] SPOT 513: pI=6.16; Mw=36631   [identification data] SPOT 514: pI=6.16; Mw=36631   [identification data] IDENTIFICATION: SPOT 348: SeqCov=37.7%. Peptides MS=12/20. Mascot PMF Score=103 [1] SPOT 440: SeqCov=27.2%. Peptides MS=9/20. Mascot PMF Score=63 [1]; SPOT 513: SeqCov=32%. Peptides MS=10/20. Mascot PMF Score=77 [1]; SPOT 514: SeqCov=40.1%. Peptides MS=14/20. Mascot PMF Score=122 [1]. PEPTIDE EVIDENCE: SPOT 348: 221-GEFITTVQQR-230 (68) / 299-FVEGLPINDFSR-310 (77) / 180-NVIIWGNHSSTQYPDVNHAK-199 (95) [1] SPOT 440: 221-GEFITTVQQR-230 (62) / 299-FVEGLPINDFSR-310 (88) [1]; SPOT 513: 221-GEFITTVQQR-230 (49) / 299-FVEGLPINDFSR-310 (69) [1]. MAPPING (identification): SPOT 348: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 440: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 513: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 514: Peptide mass fingerprinting [1]. Copyright Data from Dr. Jatin Burniston, Liverpool John Moores University, UK Cross-references UniProtKB/Swiss-Prot O88989; MDHC_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name MDHC_RAT Primary accession number O88989 Secondary accession number(s) O88585 Q6PCV2 Sequence was last modified on  January 23, 2007 (version 3) Annotations were last modified on  November 16, 2011 (version 84) Name and origin of the protein Description RecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase; Gene name Name=Mdh1 Synonyms=Mdh Encoded on Name=Mdh1; Synonyms=Mdh Keywords Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL AF093773; AAC64180.1; -; mRNA EMBL BC059124; AAH59124.1; -; mRNA EMBL AF075574; AAC26799.1; -; mRNA IPI IPI00198717; -; . RefSeq NP_150238.1; NM_033235.1; . UniGene Rn.13492; -; . ProteinModelPortal O88989; -; . SMR O88989; 2-334; . IntAct O88989; 1; . STRING O88989; -; . PhosphoSite O88989; -; . World-2DPAGE 0004:O88989; -; . PRIDE O88989; -; . Ensembl ENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; . GeneID 24551; -; . KEGG rno:24551; -; . NMPDR fig|10116.3.peg.10844; -; . UCSC NM_033235; rat; . CTD 4190; -; . RGD 3072; Mdh1; . eggNOG roNOG11216; -; . GeneTree ENSGT00530000063410; -; . HOVERGEN HBG006340; -; . InParanoid O88989; -; . OrthoDB EOG4CVG78; -; . PhylomeDB O88989; -; . NextBio 603654; -; . ArrayExpress O88989; -; . Genevestigator O88989; -; . GermOnline ENSRNOG00000008103; Rattus norvegicus; . GO GO:0005625; C:soluble fraction; IDA:RGD; . GO GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; . GO GO:0051287; F:NAD binding; IDA:RGD; . GO GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; . GO GO:0006108; P:malate metabolic process; IDA:RGD; . GO GO:0006734; P:NADH metabolic process; IDA:RGD; . GO GO:0006107; P:oxaloacetate metabolic process; IDA:RGD; . GO GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; . InterPro IPR001557; L-lactate/malate_DH; . InterPro IPR022383; Lactate/malate_DH_C; . InterPro IPR001236; Lactate/malate_DH_N; . InterPro IPR015955; Lactate_DH/Glyco_Ohase_4_C; . InterPro IPR001252; Malate_DH_AS; . InterPro IPR011274; Malate_DH_NAD-dep_euk; . InterPro IPR010945; Malate_DH_type2; . InterPro IPR016040; NAD(P)-bd_dom; . Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1; . Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1; . KO K00025; -; . PANTHER PTHR23382; MDH_SF1; 1; . Pfam PF02866; Ldh_1_C; 1; . Pfam PF00056; Ldh_1_N; 1; . PIRSF PIRSF000102; Lac_mal_DH; 1; . SUPFAM SSF56327; Lactate_DH/Glyco_hydro_4_C; 1; . TIGRFAMs TIGR01759; MalateDH-SF1; 1; . TIGRFAMs TIGR01758; MDH_euk_cyt; 1; . PROSITE PS00068; MDH; 1; . World-2DPAGE Repository (search AC)