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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: NEC2_RAT World-2DPAGE Repository (0030):  NEC2_RAT NEC2_RAT General information about the entry View entry in simple text format Entry name NEC2_RAT Primary accession number P28841 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Neuroendocrine convertase 2; Short=NEC 2; EC=3.4.21.94; AltName: Full=KEX2-like endoprotease 2; AltName: Full=Prohormone convertase 2; AltName: Full=Proprotein convertase 2; Short=PC2; Flags: Precursor;. Gene name Name=Pcsk2 Synonyms=Nec-2, Nec2, Rpc2 Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 610: pI=4.99; Mw=76087   [identification data] SPOT 624: pI=4.85; Mw=76721   [identification data] SPOT 625: pI=4.92; Mw=76298   [identification data] SPOT 632: pI=5.07; Mw=75877   [identification data] SPOT 661: pI=4.78; Mw=74421   [identification data] SPOT 680: pI=4.85; Mw=74011   [identification data] SPOT 694: pI=4.92; Mw=73501   [identification data] IDENTIFICATION: SPOT 610: Mascot protein score=168. Number of peptides=5 [1] SPOT 624: Mascot protein score=70. Number of peptides=4 [1]; SPOT 625: Mascot protein score=78. Number of peptides=4 [1]; SPOT 632: Mascot protein score=66. Number of peptides=4 [1]; SPOT 661: Mascot protein score=206. Number of peptides=6 [1]; SPOT 680: Mascot protein score=201. Number of peptides=7 [1]; SPOT 694: Mascot protein score=113. Number of peptides=3 [1]. MAPPING (identification): SPOT 610: Tandem mass spectrometry [1]; SPOT 624: Tandem mass spectrometry [1]; SPOT 625: Tandem mass spectrometry [1]; SPOT 632: Tandem mass spectrometry [1]; SPOT 661: Tandem mass spectrometry [1]; SPOT 680: Tandem mass spectrometry [1]; SPOT 694: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot P28841; NEC2_RAT. World-2DPAGE Repository P28841; NEC2_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name NEC2_RAT Primary accession number P28841 Sequence was last modified on  December 1, 1992 (version 1) Annotations were last modified on  November 16, 2011 (version 99) Name and origin of the protein Description RecName: Full=Neuroendocrine convertase 2; Short=NEC 2; EC=3.4.21.94; AltName: Full=KEX2-like endoprotease 2; AltName: Full=Prohormone convertase 2; AltName: Full=Proprotein convertase 2; Short=PC2; Flags: Precursor; Gene name Name=Pcsk2 Synonyms=Nec-2, Nec2, Rpc2 Encoded on Name=Pcsk2; Synonyms=Nec-2, Nec2, Rpc2 Keywords Cleavage on pair of basic residues; Complete proteome; Cytoplasmic vesicle; Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome; Serine protease; Signal; Zymogen. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M76706; AAA40946.1; -; mRNA EMBL M83746; AAA41477.1; -; mRNA IPI IPI00204864; -; . PIR B41556; KXRTC2; . RefSeq NP_036878.1; NM_012746.1; . UniGene Rn.89052; -; . ProteinModelPortal P28841; -; . SMR P28841; 119-596; . IntAct P28841; 1; . STRING P28841; -; . MEROPS S08.073; -; . PRIDE P28841; -; . Ensembl ENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438; . GeneID 25121; -; . KEGG rno:25121; -; . UCSC NM_012746; rat; . CTD 5126; -; . RGD 3273; Pcsk2; . eggNOG roNOG07042; -; . GeneTree ENSGT00600000084064; -; . HOVERGEN HBG008705; -; . InParanoid P28841; -; . OMA NYNADAS; -; . OrthoDB EOG46MBJ1; -; . PhylomeDB P28841; -; . NextBio 605503; -; . ArrayExpress P28841; -; . Genevestigator P28841; -; . GermOnline ENSRNOG00000005438; Rattus norvegicus; . GO GO:0005615; C:extracellular space; IBA:RefGenome; . GO GO:0030133; C:transport vesicle; IEA:UniProtKB-SubCell; . GO GO:0005515; F:protein binding; IPI:IntAct; . GO GO:0004252; F:serine-type endopeptidase activity; IMP:BHF-UCL; . GO GO:0034231; P:islet amyloid polypeptide processing; IMP:BHF-UCL; . GO GO:0006508; P:proteolysis; IMP:RGD; . InterPro IPR008979; Galactose-bd-like; . InterPro IPR000209; Peptidase_S8/S53; . InterPro IPR023827; Peptidase_S8_Asp-AS; . InterPro IPR022398; Peptidase_S8_His-AS; . InterPro IPR023828; Peptidase_S8_Ser-AS; . InterPro IPR015500; Peptidase_S8_subtilisin-rel; . InterPro IPR009020; Prot_inh_propept; . InterPro IPR002884; PrprotnconvertsP; . Gene3D G3DSA:3.40.50.200; Pept_S8_S53; 1; . KO K01360; -; . PANTHER PTHR10795; SubtilSerProt; 1; . Pfam PF01483; P_proprotein; 1; . Pfam PF00082; Peptidase_S8; 1; . PRINTS PR00723; SUBTILISIN; . SUPFAM SSF49785; Gal_bind_like; 1; . SUPFAM SSF52743; Pept_S8_S53; 1; . SUPFAM SSF54897; Prot_inh_propept; 1; . PROSITE PS00136; SUBTILASE_ASP; 1; . PROSITE PS00137; SUBTILASE_HIS; 1; . PROSITE PS00138; SUBTILASE_SER; 1; . 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