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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: NSF1C_RAT World-2DPAGE Repository (0030):  NSF1C_RAT NSF1C_RAT General information about the entry View entry in simple text format Entry name NSF1C_RAT Primary accession number O35987 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=NSFL1 cofactor p47; AltName: Full=XY body-associated protein XY40; AltName: Full=p97 cofactor p47;. Gene name Name=Nsfl1c Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 1205: pI=4.98; Mw=55813   [identification data] SPOT 1218: pI=5.13; Mw=55198   [identification data] SPOT 1223: pI=5.00; Mw=55046   [identification data] SPOT 1230: pI=4.86; Mw=54894   [identification data] SPOT 1236: pI=4.94; Mw=54894   [identification data] SPOT 1245: pI=5.06; Mw=54365   [identification data] SPOT 3028: pI=4.90; Mw=54742   [identification data] IDENTIFICATION: SPOT 1205: Mascot protein score=210. Number of peptides=4 [1] SPOT 1218: Mascot protein score=60. Number of peptides=3 [1]; SPOT 1223: Mascot protein score=397. Number of peptides=7 [1]; SPOT 1230: Mascot protein score=122. Number of peptides=5 [1]; SPOT 1236: Mascot protein score=210. Number of peptides=4 [1]; SPOT 1245: Mascot protein score=419. Number of peptides=8 [1]; SPOT 3028: Mascot protein score=642. Number of peptides=11 [1]. MAPPING (identification): SPOT 1205: Tandem mass spectrometry [1]; SPOT 1218: Tandem mass spectrometry [1]; SPOT 1223: Tandem mass spectrometry [1]; SPOT 1230: Tandem mass spectrometry [1]; SPOT 1236: Tandem mass spectrometry [1]; SPOT 1245: Tandem mass spectrometry [1]; SPOT 3028: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot O35987; NSF1C_RAT. World-2DPAGE Repository O35987; NSF1C_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name NSF1C_RAT Primary accession number O35987 Sequence was last modified on  January 1, 1998 (version 1) Annotations were last modified on  November 16, 2011 (version 99) Name and origin of the protein Description RecName: Full=NSFL1 cofactor p47; AltName: Full=XY body-associated protein XY40; AltName: Full=p97 cofactor p47; Gene name Name=Nsfl1c Encoded on Name=Nsfl1c Keywords 3D-structure; Chromosome; Complete proteome; Direct protein sequencing; Golgi apparatus; Lipid-binding; Nucleus; Phosphoprotein; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL Y10769; CAA71742.1; -; mRNA EMBL AB002086; BAA21659.1; -; mRNA EMBL BC072464; AAH72464.1; -; mRNA IPI IPI00214262; -; . RefSeq NP_114187.1; NM_031981.1; . UniGene Rn.2771; -; . PDB 1I42; NMR; -; A=282-370 PDB 1JRU; NMR; -; A=282-370 PDB 1S3S; X-ray; 2.90 A; G/H/I=244-370 PDB 1V92; NMR; -; A=1-46 PDB 1VAZ; NMR; -; A=171-246 PDBsum 1I42; -; . PDBsum 1JRU; -; . PDBsum 1S3S; -; . PDBsum 1V92; -; . PDBsum 1VAZ; -; . ProteinModelPortal O35987; -; . SMR O35987; 1-46; 171-246; 253-370 IntAct O35987; 15; . MINT MINT-1954288; -; . STRING O35987; -; . PhosphoSite O35987; -; . PRIDE O35987; -; . Ensembl ENSRNOT00000011654; ENSRNOP00000011654; ENSRNOG00000008604; . GeneID 83809; -; . KEGG rno:83809; -; . UCSC NM_031981; rat; . CTD 55968; -; . RGD 619952; Nsfl1c; . eggNOG roNOG06792; -; . GeneTree ENSGT00520000055567; -; . HOVERGEN HBG054517; -; . PhylomeDB O35987; -; . NextBio 616417; -; . ArrayExpress O35987; -; . Genevestigator O35987; -; . GermOnline ENSRNOG00000008604; Rattus norvegicus; . GO GO:0005694; C:chromosome; IEA:UniProtKB-SubCell; . GO GO:0005829; C:cytosol; IDA:RGD; . GO GO:0005795; C:Golgi stack; IEA:UniProtKB-SubCell; . GO GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; . GO GO:0051117; F:ATPase binding; IPI:RGD; . GO GO:0008289; F:lipid binding; IEA:UniProtKB-KW; . GO GO:0043130; F:ubiquitin binding; IDA:BHF-UCL; . GO GO:0007030; P:Golgi organization; IDA:RGD; . GO GO:0061025; P:membrane fusion; IDA:RGD; . InterPro IPR012989; SEP_domain; . InterPro IPR009060; UBA-like; . InterPro IPR001012; UBX; . KO K14012; -; . Pfam PF08059; SEP; 1; . Pfam PF00789; UBX; 1; . SMART SM00553; SEP; 1; . SMART SM00166; UBX; 1; . SUPFAM SSF102848; SEP; 1; . SUPFAM SSF46934; UBA_like; 1; . PROSITE PS51399; SEP; 1; . PROSITE PS50033; UBX; 1; . World-2DPAGE Repository (search AC)