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Searching in 'World-2DPAGE Repository [0019]' for entry matching: NSF_RAT




World-2DPAGE Repository (0019):  NSF_RAT


NSF_RAT


General information about the entry
View entry in simple text format
Entry nameNSF_RAT
Primary accession numberQ9QUL6
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Vesicle-fusing ATPase; EC=3.6.4.6; AltName: Full=N-ethylmaleimide-sensitive fusion protein; Short=NEM-sensitive fusion protein; AltName: Full=Vesicular-fusion protein NSF;.
Gene nameName=Nsf
Synonyms=Erg1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1111/j.1471-4159.2010.06719.x;
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
HIPP_SYNAP
  map experimental info
 
HIPP_SYNAP

MAP LOCATIONS:
pI=6.55; Mw=83170  [identification data]
pI=6.55; Mw=83170  [identification data]
pI=6.55; Mw=83170  [identification data]
pI=6.55; Mw=83170  [identification data]
pI=6.55; Mw=83170  [identification data]

IDENTIFICATION: SPOT 392: Peptides number=9. SeqCov=11.3%. MOWSE score=1.08E+6 [1]
SPOT 414: Peptides number=7. SeqCov=9%. MOWSE score=1.06E+5 [1]; SPOT 416: Peptides number=8. SeqCov=11.2%. MOWSE score=1.88E+6 [1]; SPOT 418: Peptides number=16. SeqCov=21.9%. MOWSE score=4.36E+12 [1]; SPOT 1004: Peptides number=14. SeqCov=20%. MOWSE score=9.20E+10 [1].
MAPPING (identification):
SPOT 392: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 414: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 416: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 418: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1004: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Willard Freeman, Penn State College of Medicine, USA
Cross-references
UniProtKB/Swiss-ProtQ9QUL6; NSF_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameNSF_RAT
Primary accession numberQ9QUL6
Sequence was last modified on May 1, 2000 (version 1)
Annotations were last modified on December 14, 2011 (version 80)
Name and origin of the protein
DescriptionRecName: Full=Vesicle-fusing ATPase; EC=3.6.4.6; AltName: Full=N-ethylmaleimide-sensitive fusion protein; Short=NEM-sensitive fusion protein; AltName: Full=Vesicular-fusion protein NSF;
Gene nameName=Nsf
Synonyms=Erg1
Encoded onName=Nsf; Synonyms=Erg1
KeywordsATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF142097; AAD39485.1; -; mRNA
EMBLAF189019; AAF01051.1; -; mRNA
IPIIPI00210635; -; .
RefSeqNP_068516.1; NM_021748.1; .
UniGeneRn.13345; -; .
HSSPP18708; 1NSF; .
ProteinModelPortalQ9QUL6; -; .
SMRQ9QUL6; 1-201; 489-735; .
IntActQ9QUL6; 3; .
STRINGQ9QUL6; -; .
PhosphoSiteQ9QUL6; -; .
World-2DPAGE0004:Q9QUL6; -; .
PRIDEQ9QUL6; -; .
GeneID60355; -; .
KEGGrno:60355; -; .
CTD4905; -; .
RGD621594; Nsf; .
GeneTreeENSGT00530000064085; -; .
HOVERGENHBG000324; -; .
InParanoidQ9QUL6; -; .
OrthoDBEOG4DZ1TR; -; .
PhylomeDBQ9QUL6; -; .
NextBio612027; -; .
ArrayExpressQ9QUL6; -; .
GenevestigatorQ9QUL6; -; .
GermOnlineENSRNOG00000003905; Rattus norvegicus; .
GOGO:0043198; C:dendritic shaft; IDA:UniProtKB; .
GOGO:0005795; C:Golgi stack; IDA:RGD; .
GOGO:0014069; C:postsynaptic density; TAS:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0008022; F:protein C-terminus binding; IPI:UniProtKB; .
GOGO:0017137; F:Rab GTPase binding; IDA:RGD; .
GOGO:0007269; P:neurotransmitter secretion; TAS:UniProtKB; .
GOGO:0015031; P:protein transport; IMP:RGD; .
GOGO:0017157; P:regulation of exocytosis; IMP:RGD; .
GOGO:0006906; P:vesicle fusion; TAS:UniProtKB; .
InterProIPR009010; Asp_de-COase-like_fold; .
InterProIPR003593; ATPase_AAA+_core; .
InterProIPR003959; ATPase_AAA_core; .
InterProIPR003960; ATPase_AAA_CS; .
InterProIPR003338; ATPase_AAA_VAT_N; .
InterProIPR004201; Cell_div_Cdc48_dom2; .
Gene3DG3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1; .
KOK06027; -; .
PfamPF00004; AAA; 2; .
PfamPF02933; CDC48_2; 1; .
PfamPF02359; CDC48_N; 1; .
SMARTSM00382; AAA; 2; .
SUPFAMSSF50692; Asp_decarb_fold; 1; .
PROSITEPS00674; AAA; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server