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Searching in 'World-2DPAGE Repository [0030]' for entry matching: NUCB2_RAT




World-2DPAGE Repository (0030):  NUCB2_RAT


NUCB2_RAT


General information about the entry
View entry in simple text format
Entry nameNUCB2_RAT
Primary accession numberQ9JI85
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Nucleobindin-2; AltName: Full=DNA-binding protein NEFA; Flags: Precursor;.
Gene nameName=Nucb2
Synonyms=Nefa
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=4.91; Mw=61743  [identification data]
pI=5.97; Mw=30789  [identification data]

IDENTIFICATION: SPOT 1035: Mascot protein score=163. Number of peptides=4 [1]
SPOT 2025: Mascot protein score=307. Number of peptides=9 [1].
MAPPING (identification):
SPOT 1035: Tandem mass spectrometry [1];
SPOT 2025: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtQ9JI85; NUCB2_RAT.
World-2DPAGE RepositoryQ9JI85; NUCB2_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameNUCB2_RAT
Primary accession numberQ9JI85
Sequence was last modified on October 1, 2000 (version 1)
Annotations were last modified on September 21, 2011 (version 74)
Name and origin of the protein
DescriptionRecName: Full=Nucleobindin-2; AltName: Full=DNA-binding protein NEFA; Flags: Precursor;
Gene nameName=Nucb2
Synonyms=Nefa
Encoded onName=Nucb2; Synonyms=Nefa
KeywordsCalcium; Complete proteome; Cytoplasm; DNA-binding; Membrane; Reference proteome; Repeat; Secreted; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF250142; AAF75993.1; -; mRNA
EMBLAF238223; AAK66864.1; -; mRNA
EMBLBC061778; AAH61778.1; -; mRNA
IPIIPI00200070; -; .
RefSeqNP_067695.1; NM_021663.2; .
UniGeneRn.41602; -; .
ProteinModelPortalQ9JI85; -; .
SMRQ9JI85; 229-327; .
IntActQ9JI85; 1; .
STRINGQ9JI85; -; .
PhosphoSiteQ9JI85; -; .
PRIDEQ9JI85; -; .
GeneID59295; -; .
KEGGrno:59295; -; .
UCSCNM_021663; rat; .
CTD4925; -; .
RGD620888; Nucb2; .
eggNOGroNOG09966; -; .
GeneTreeENSGT00390000001927; -; .
HOVERGENHBG052685; -; .
InParanoidQ9JI85; -; .
OrthoDBEOG4N04F6; -; .
NextBio611821; -; .
ArrayExpressQ9JI85; -; .
GenevestigatorQ9JI85; -; .
GermOnlineENSRNOG00000020456; Rattus norvegicus; .
GOGO:0005576; C:extracellular region; IEA:UniProtKB-SubCell; .
GOGO:0005797; C:Golgi medial cisterna; IDA:RGD; .
GOGO:0003677; F:DNA binding; IEA:UniProtKB-KW; .
GOGO:0005164; F:tumor necrosis factor receptor binding; IPI:RGD; .
InterProIPR011992; EF-hand-like_dom; .
InterProIPR018247; EF_Hand_1_Ca_BS; .
InterProIPR018249; EF_HAND_2; .
InterProIPR002048; EF_hand_Ca-bd; .
Gene3DG3DSA:1.10.238.10; EF-Hand_type; 1; .
SMARTSM00054; EFh; 2; .
PROSITEPS00018; EF_HAND_1; 2; .
PROSITEPS50222; EF_HAND_2; 2; .



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