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Searching in 'World-2DPAGE Repository [0030]' for entry matching: O08583




World-2DPAGE Repository (0030):  O08583

O08583


General information about the entry
View entry in simple text format
Entry nameTHOC4_MOUSE
Primary accession numberO08583
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=THO complex subunit 4; Short=Tho4; AltName: Full=Ally of AML-1 and LEF-1; AltName: Full=REF1-I; AltName: Full=RNA and export factor-binding protein 1; AltName: Full=Transcriptional coactivator Aly/REF;.
Gene nameName=Thoc4
Synonyms=Aly, Ref1, Refbp1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.52; Mw=34583  [identification data]
pI=6.10; Mw=33407  [identification data]

IDENTIFICATION: SPOT 1880: Mascot protein score=55. Number of peptides=1 [1]
SPOT 1929: Mascot protein score=42. Number of peptides=1 [1].
MAPPING (identification):
SPOT 1880: Tandem mass spectrometry [1];
SPOT 1929: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtO08583; THOC4_MOUSE.
World-2DPAGE RepositoryO08583; THOC4_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameTHOC4_MOUSE
Primary accession numberO08583
Secondary accession number(s) Q0VBL5 Q8CBM4 Q9JJW7
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 103)
Name and origin of the protein
DescriptionRecName: Full=THO complex subunit 4; Short=Tho4; AltName: Full=Ally of AML-1 and LEF-1; AltName: Full=REF1-I; AltName: Full=RNA and export factor-binding protein 1; AltName: Full=Transcriptional coactivator Aly/REF;
Gene nameName=Thoc4
Synonyms=Aly, Ref1, Refbp1
Encoded onName=Thoc4; Synonyms=Aly, Ref1, Refbp1
Keywords3D-structure; Acetylation; Alternative splicing; Chaperone; Complete proteome; Cytoplasm; Methylation; mRNA processing; mRNA splicing; mRNA transport; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU89876; AAC53117.1; -; mRNA
EMBLAJ252140; CAB76383.1; -; mRNA
EMBLAL663030; CAM27086.1; -; Genomic_DNA
EMBLCH466558; EDL34770.1; -; Genomic_DNA
EMBLBC120588; AAI20589.1; -; mRNA
EMBLBC137658; AAI37659.1; -; mRNA
EMBLAK035721; BAC29168.1; -; mRNA
IPIIPI00114407; -; .
IPIIPI00122339; -; .
RefSeqNP_035698.1; NM_011568.1; .
UniGeneMm.1886; -; .
PDB1NO8; NMR; -; A=77-182
PDBsum1NO8; -; .
ProteinModelPortalO08583; -; .
SMRO08583; 1-187; .
STRINGO08583; -; .
PhosphoSiteO08583; -; .
PRIDEO08583; -; .
EnsemblENSMUST00000026125; ENSMUSP00000026125; ENSMUSG00000025134; .
GeneID21681; -; .
KEGGmmu:21681; -; .
UCSCuc007mtl.1; mouse; .
CTD10189; -; .
MGIMGI:1341044; Thoc4; .
eggNOGroNOG17249; -; .
GeneTreeENSGT00410000025615; -; .
HOGENOMHBG608782; -; .
HOVERGENHBG054806; -; .
InParanoidO08583; -; .
OMANGNFNGN; -; .
OrthoDBEOG4MW87F; -; .
NextBio300988; -; .
ArrayExpressO08583; -; .
BgeeO08583; -; .
CleanExMM_THOC4; -; .
GenevestigatorO08583; -; .
GermOnlineENSMUSG00000025134; Mus musculus; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell; .
GOGO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW; .
GOGO:0000166; F:nucleotide binding; IEA:InterPro; .
GOGO:0003723; F:RNA binding; IDA:MGI; .
GOGO:0003697; F:single-stranded DNA binding; IDA:MGI; .
GOGO:0006397; P:mRNA processing; IEA:UniProtKB-KW; .
GOGO:0051028; P:mRNA transport; IEA:UniProtKB-KW; .
GOGO:0008380; P:RNA splicing; IEA:UniProtKB-KW; .
InterProIPR012677; Nucleotide-bd_a/b_plait; .
InterProIPR000504; RRM_dom; .
Gene3DG3DSA:3.30.70.330; a_b_plait_nuc_bd; 1; .
KOK12881; -; .
PfamPF00076; RRM_1; 1; .
SMARTSM00360; RRM; 1; .
PROSITEPS50102; RRM; 1; .


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