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Searching in 'World-2DPAGE Repository [0030]' for entry matching: O35077




World-2DPAGE Repository (0030):  O35077


O35077


General information about the entry
View entry in simple text format
Entry nameGPDA_RAT
Primary accession numberO35077
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; Short=GPD-C; Short=GPDH-C; EC=1.1.1.8;.
Gene nameName=Gpd1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.37; Mw=43691  [identification data]

IDENTIFICATION: SPOT 1580: Mascot protein score=143. Number of peptides=6 [1].
MAPPING (identification):
SPOT 1580: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtO35077; GPDA_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameGPDA_RAT
Primary accession numberO35077
Secondary accession number(s) Q5I0F4
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on November 16, 2011 (version 93)
Name and origin of the protein
DescriptionRecName: Full=Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; Short=GPD-C; Short=GPDH-C; EC=1.1.1.8;
Gene nameName=Gpd1
Encoded onName=Gpd1
KeywordsComplete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB002558; BAA21763.1; -; mRNA
EMBLBC088396; AAH88396.1; -; mRNA
IPIIPI00231148; -; .
RefSeqNP_071551.2; NM_022215.2; .
UniGeneRn.44452; -; .
ProteinModelPortalO35077; -; .
SMRO35077; 1-349; .
STRINGO35077; -; .
PRIDEO35077; -; .
EnsemblENSRNOT00000026199; ENSRNOP00000026200; ENSRNOG00000019213; .
GeneID60666; -; .
KEGGrno:60666; -; .
NMPDRfig|10116.3.peg.27756; -; .
UCSCNM_022215; rat; .
CTD2819; -; .
RGD621381; Gpd1; .
eggNOGroNOG13541; -; .
GeneTreeENSGT00390000003114; -; .
HOVERGENHBG003669; -; .
InParanoidO35077; -; .
OMAEEDIGGK; -; .
OrthoDBEOG4WQ12X; -; .
PhylomeDBO35077; -; .
NextBio612385; -; .
ArrayExpressO35077; -; .
GenevestigatorO35077; -; .
GermOnlineENSRNOG00000019213; Rattus norvegicus; .
GOGO:0005829; C:cytosol; IDA:RGD; .
GOGO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IDA:RGD; .
GOGO:0004368; F:glycerol-3-phosphate dehydrogenase activity; IDA:RGD; .
GOGO:0051287; F:NAD binding; IDA:RGD; .
GOGO:0042803; F:protein homodimerization activity; IEA:InterPro; .
GOGO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro; .
GOGO:0046486; P:glycerolipid metabolic process; IDA:RGD; .
GOGO:0006116; P:NADH oxidation; IDA:RGD; .
InterProIPR008927; 6-PGluconate_DH_C-like; .
InterProIPR013328; DH_multihelical; .
InterProIPR006168; G3P_DH_NAD-dep; .
InterProIPR006109; G3P_DH_NAD-dep_C; .
InterProIPR017751; G3P_DH_NAD-dep_euk; .
InterProIPR011128; G3P_DH_NAD-dep_N; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
Gene3DG3DSA:1.10.1040.10; Opine_DH; 1; .
KOK00006; -; .
PANTHERPTHR11728; NAD_Gly3P_DH; 1; .
PfamPF07479; NAD_Gly3P_dh_C; 1; .
PfamPF01210; NAD_Gly3P_dh_N; 1; .
PIRSFPIRSF000114; Glycerol-3-P_dh; 1; .
PRINTSPR00077; GPDHDRGNASE; .
SUPFAMSSF48179; 6DGDH_C_like; 1; .
TIGRFAMsTIGR03376; Glycerol3P_DH; 1; .
PROSITEPS00957; NAD_G3PDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server