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Searching in 'World-2DPAGE Repository [0030]' for entry matching: O35987

World-2DPAGE Repository (0030):  O35987


General information about the entry
View entry in simple text format
Entry nameNSF1C_RAT
Primary accession numberO35987
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=NSFL1 cofactor p47; AltName: Full=XY body-associated protein XY40; AltName: Full=p97 cofactor p47;.
Gene nameName=Nsfl1c
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
  map experimental info

pI=4.98; Mw=55813  [identification data]
pI=5.13; Mw=55198  [identification data]
pI=5.00; Mw=55046  [identification data]
pI=4.86; Mw=54894  [identification data]
pI=4.94; Mw=54894  [identification data]
pI=5.06; Mw=54365  [identification data]
pI=4.90; Mw=54742  [identification data]

IDENTIFICATION: SPOT 1205: Mascot protein score=210. Number of peptides=4 [1]
SPOT 1218: Mascot protein score=60. Number of peptides=3 [1]; SPOT 1223: Mascot protein score=397. Number of peptides=7 [1]; SPOT 1230: Mascot protein score=122. Number of peptides=5 [1]; SPOT 1236: Mascot protein score=210. Number of peptides=4 [1]; SPOT 1245: Mascot protein score=419. Number of peptides=8 [1]; SPOT 3028: Mascot protein score=642. Number of peptides=11 [1].
MAPPING (identification):
SPOT 1205: Tandem mass spectrometry [1];
SPOT 1218: Tandem mass spectrometry [1];
SPOT 1223: Tandem mass spectrometry [1];
SPOT 1230: Tandem mass spectrometry [1];
SPOT 1236: Tandem mass spectrometry [1];
SPOT 1245: Tandem mass spectrometry [1];
SPOT 3028: Tandem mass spectrometry [1].

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
UniProtKB/Swiss-ProtO35987; NSF1C_RAT.
World-2DPAGE RepositoryO35987; NSF1C_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameNSF1C_RAT
Primary accession numberO35987
Sequence was last modified on January 1, 1998 (version 1)
Annotations were last modified on November 16, 2011 (version 99)
Name and origin of the protein
DescriptionRecName: Full=NSFL1 cofactor p47; AltName: Full=XY body-associated protein XY40; AltName: Full=p97 cofactor p47;
Gene nameName=Nsfl1c
Encoded onName=Nsfl1c
Keywords3D-structure; Chromosome; Complete proteome; Direct protein sequencing; Golgi apparatus; Lipid-binding; Nucleus; Phosphoprotein; Reference proteome.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLY10769; CAA71742.1; -; mRNA
EMBLAB002086; BAA21659.1; -; mRNA
EMBLBC072464; AAH72464.1; -; mRNA
IPIIPI00214262; -; .
RefSeqNP_114187.1; NM_031981.1; .
UniGeneRn.2771; -; .
PDB1I42; NMR; -; A=282-370
PDB1JRU; NMR; -; A=282-370
PDB1S3S; X-ray; 2.90 A; G/H/I=244-370
PDB1V92; NMR; -; A=1-46
PDB1VAZ; NMR; -; A=171-246
PDBsum1I42; -; .
PDBsum1JRU; -; .
PDBsum1S3S; -; .
PDBsum1V92; -; .
PDBsum1VAZ; -; .
ProteinModelPortalO35987; -; .
SMRO35987; 1-46; 171-246; 253-370
IntActO35987; 15; .
MINTMINT-1954288; -; .
STRINGO35987; -; .
PhosphoSiteO35987; -; .
PRIDEO35987; -; .
EnsemblENSRNOT00000011654; ENSRNOP00000011654; ENSRNOG00000008604; .
GeneID83809; -; .
KEGGrno:83809; -; .
UCSCNM_031981; rat; .
CTD55968; -; .
RGD619952; Nsfl1c; .
eggNOGroNOG06792; -; .
GeneTreeENSGT00520000055567; -; .
HOVERGENHBG054517; -; .
PhylomeDBO35987; -; .
NextBio616417; -; .
ArrayExpressO35987; -; .
GenevestigatorO35987; -; .
GermOnlineENSRNOG00000008604; Rattus norvegicus; .
GOGO:0005694; C:chromosome; IEA:UniProtKB-SubCell; .
GOGO:0005829; C:cytosol; IDA:RGD; .
GOGO:0005795; C:Golgi stack; IEA:UniProtKB-SubCell; .
GOGO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GOGO:0051117; F:ATPase binding; IPI:RGD; .
GOGO:0008289; F:lipid binding; IEA:UniProtKB-KW; .
GOGO:0043130; F:ubiquitin binding; IDA:BHF-UCL; .
GOGO:0007030; P:Golgi organization; IDA:RGD; .
GOGO:0061025; P:membrane fusion; IDA:RGD; .
InterProIPR012989; SEP_domain; .
InterProIPR009060; UBA-like; .
InterProIPR001012; UBX; .
KOK14012; -; .
PfamPF08059; SEP; 1; .
PfamPF00789; UBX; 1; .
SMARTSM00553; SEP; 1; .
SMARTSM00166; UBX; 1; .
SUPFAMSSF102848; SEP; 1; .
SUPFAMSSF46934; UBA_like; 1; .
PROSITEPS51399; SEP; 1; .
PROSITEPS50033; UBX; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server