Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 85:

pI=5.36; Mw=95000

MAPPING (identification):

SPOT 85: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

View entry in simple text format

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	HSP74_RAT
Primary accession number	O88600
Sequence was last modified on	November 1, 1998 (version 1)
Annotations were last modified on	November 16, 2011 (version 80)
Name and origin of the protein
Description	RecName: Full=Heat shock 70 kDa protein 4; AltName: Full=Ischemia responsive 94 kDa protein;
Gene name	Name=Hspa4 Synonyms=Irp94
Encoded on	Name=Hspa4; Synonyms=Irp94
Keywords	Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AF077354; AAC27937.1; -; mRNA
IPI	IPI00387868; -; .
RefSeq	NP_705893.1; NM_153629.1; .
UniGene	Rn.163092; -; .
HSSP	P19120; 1BA1; .
ProteinModelPortal	O88600; -; .
MINT	MINT-4566747; -; .
STRING	O88600; -; .
PhosphoSite	O88600; -; .
World-2DPAGE	0004:O88600; -; .
PRIDE	O88600; -; .
GeneID	266759; -; .
KEGG	rno:266759; -; .
UCSC	NM_153629; rat; .
CTD	3308; -; .
RGD	628878; Hspa4; .
eggNOG	maNOG07919; -; .
GeneTree	ENSGT00390000016919; -; .
HOVERGEN	HBG047955; -; .
InParanoid	O88600; -; .
OrthoDB	EOG415GCS; -; .
PhylomeDB	O88600; -; .
NextBio	624599; -; .
ArrayExpress	O88600; -; .
Genevestigator	O88600; -; .
GermOnline	ENSRNOG00000016596; Rattus norvegicus; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0005811; C:lipid particle; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0032092; P:positive regulation of protein binding; IDA:RGD; .
GO	GO:0009408; P:response to heat; IDA:RGD; .
InterPro	IPR018181; Heat_shock_70_CS; .
InterPro	IPR001023; Hsp70; .
InterPro	IPR013126; Hsp_70; .
KO	K09489; -; .
PANTHER	PTHR19375; Hsp70; 1; .
Pfam	PF00012; HSP70; 2; .
PRINTS	PR00301; HEATSHOCK70; .
PROSITE	PS00297; HSP70_1; FALSE_NEG; .
PROSITE	PS00329; HSP70_2; 1; .
PROSITE	PS01036; HSP70_3; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: O88600

World-2DPAGE Repository (0014): O88600

O88600

General information about the entry

Entry name

HSP74_RAT

Primary accession number

O88600

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Heat shock 70 kDa protein 4; AltName: Full=Ischemia responsive 94 kDa protein;.

Gene name

Name=Hspa4
Synonyms=Irp94

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2683:

pI=5.35; Mw=79000

IDENTIFICATION: SPOT 2683: SeqCov=42%. MascotPMF=200 [1].
MAPPING (identification):

SPOT 2683: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

View entry in simple text format

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	HSP74_RAT
Primary accession number	O88600
Sequence was last modified on	November 1, 1998 (version 1)
Annotations were last modified on	November 16, 2011 (version 80)
Name and origin of the protein
Description	RecName: Full=Heat shock 70 kDa protein 4; AltName: Full=Ischemia responsive 94 kDa protein;
Gene name	Name=Hspa4 Synonyms=Irp94
Encoded on	Name=Hspa4; Synonyms=Irp94
Keywords	Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AF077354; AAC27937.1; -; mRNA
IPI	IPI00387868; -; .
RefSeq	NP_705893.1; NM_153629.1; .
UniGene	Rn.163092; -; .
HSSP	P19120; 1BA1; .
ProteinModelPortal	O88600; -; .
MINT	MINT-4566747; -; .
STRING	O88600; -; .
PhosphoSite	O88600; -; .
World-2DPAGE	0004:O88600; -; .
PRIDE	O88600; -; .
GeneID	266759; -; .
KEGG	rno:266759; -; .
UCSC	NM_153629; rat; .
CTD	3308; -; .
RGD	628878; Hspa4; .
eggNOG	maNOG07919; -; .
GeneTree	ENSGT00390000016919; -; .
HOVERGEN	HBG047955; -; .
InParanoid	O88600; -; .
OrthoDB	EOG415GCS; -; .
PhylomeDB	O88600; -; .
NextBio	624599; -; .
ArrayExpress	O88600; -; .
Genevestigator	O88600; -; .
GermOnline	ENSRNOG00000016596; Rattus norvegicus; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0005811; C:lipid particle; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0032092; P:positive regulation of protein binding; IDA:RGD; .
GO	GO:0009408; P:response to heat; IDA:RGD; .
InterPro	IPR018181; Heat_shock_70_CS; .
InterPro	IPR001023; Hsp70; .
InterPro	IPR013126; Hsp_70; .
KO	K09489; -; .
PANTHER	PTHR19375; Hsp70; 1; .
Pfam	PF00012; HSP70; 2; .
PRINTS	PR00301; HEATSHOCK70; .
PROSITE	PS00297; HSP70_1; FALSE_NEG; .
PROSITE	PS00329; HSP70_2; 1; .
PROSITE	PS01036; HSP70_3; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: O88600

World-2DPAGE Repository (0019): O88600

O88600

General information about the entry

Entry name

HSP74_RAT

Primary accession number

O88600

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

RecName: Full=Heat shock 70 kDa protein 4; AltName: Full=Ischemia responsive 94 kDa protein;.

Gene name

Name=Hspa4
Synonyms=Irp94

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 990:

pI=5.13; Mw=94795

IDENTIFICATION: SPOT 990: Peptides number=11. SeqCov=19.3%. MOWSE score=4.28E+11 [1].
MAPPING (identification):

SPOT 990: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

View entry in simple text format

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	HSP74_RAT
Primary accession number	O88600
Sequence was last modified on	November 1, 1998 (version 1)
Annotations were last modified on	November 16, 2011 (version 80)
Name and origin of the protein
Description	RecName: Full=Heat shock 70 kDa protein 4; AltName: Full=Ischemia responsive 94 kDa protein;
Gene name	Name=Hspa4 Synonyms=Irp94
Encoded on	Name=Hspa4; Synonyms=Irp94
Keywords	Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AF077354; AAC27937.1; -; mRNA
IPI	IPI00387868; -; .
RefSeq	NP_705893.1; NM_153629.1; .
UniGene	Rn.163092; -; .
HSSP	P19120; 1BA1; .
ProteinModelPortal	O88600; -; .
MINT	MINT-4566747; -; .
STRING	O88600; -; .
PhosphoSite	O88600; -; .
World-2DPAGE	0004:O88600; -; .
PRIDE	O88600; -; .
GeneID	266759; -; .
KEGG	rno:266759; -; .
UCSC	NM_153629; rat; .
CTD	3308; -; .
RGD	628878; Hspa4; .
eggNOG	maNOG07919; -; .
GeneTree	ENSGT00390000016919; -; .
HOVERGEN	HBG047955; -; .
InParanoid	O88600; -; .
OrthoDB	EOG415GCS; -; .
PhylomeDB	O88600; -; .
NextBio	624599; -; .
ArrayExpress	O88600; -; .
Genevestigator	O88600; -; .
GermOnline	ENSRNOG00000016596; Rattus norvegicus; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0005811; C:lipid particle; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0032092; P:positive regulation of protein binding; IDA:RGD; .
GO	GO:0009408; P:response to heat; IDA:RGD; .
InterPro	IPR018181; Heat_shock_70_CS; .
InterPro	IPR001023; Hsp70; .
InterPro	IPR013126; Hsp_70; .
KO	K09489; -; .
PANTHER	PTHR19375; Hsp70; 1; .
Pfam	PF00012; HSP70; 2; .
PRINTS	PR00301; HEATSHOCK70; .
PROSITE	PS00297; HSP70_1; FALSE_NEG; .
PROSITE	PS00329; HSP70_2; 1; .
PROSITE	PS01036; HSP70_3; 1; .

Searching in 'World-2DPAGE Repository [0030]' for entry matching: O88600

World-2DPAGE Repository (0030): O88600

O88600

General information about the entry

Entry name

HSP74_RAT

Primary accession number

O88600

integrated into World-2DPAGE Repository (0030) on

November 9, 2010 (release 1)

2D Annotations were last modified on

June 21, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Heat shock 70 kDa protein 4; AltName: Full=Ischemia responsive 94 kDa protein;.

Gene name

Name=Hspa4
Synonyms=Irp94

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011)

2D PAGE maps for identified proteins

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)

RAT_INS1E_4-7

MAP LOCATIONS:

SPOT 205:

pI=5.04; Mw=97871

SPOT 208:

pI=5.08; Mw=97736

SPOT 210:

pI=5.13; Mw=97736

SPOT 211:

pI=5.11; Mw=97601

IDENTIFICATION: SPOT 205: Mascot protein score=389. Number of peptides=11 [1]
SPOT 208: Mascot protein score=72. Number of peptides=6 [1]; SPOT 210: Mascot protein score=230. Number of peptides=7 [1]; SPOT 211: Mascot protein score=203. Number of peptides=10 [1].
MAPPING (identification):

SPOT 205: Tandem mass spectrometry [1];
SPOT 208: Tandem mass spectrometry [1];
SPOT 210: Tandem mass spectrometry [1];
SPOT 211: Tandem mass spectrometry [1].

Copyright

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium

Cross-references

UniProtKB/Swiss-Prot

World-2DPAGE Repository