resource logo

World-2DPAGE Repository

Attention: World-2DPAGE is no longer maintained.

World-2DPAGE Repository no longer accepts submissions.


World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Databases

use 'Ctrl' to select several
databases


Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal

Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0022]' for entry matching: O88989




World-2DPAGE Repository (0022):  O88989


O88989


General information about the entry
View entry in simple text format
Entry nameMDHC_RAT
Primary accession numberO88989
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.
Gene nameName=Mdh1
Synonyms=Mdh
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.16; Mw=36631  [identification data]
pI=6.16; Mw=36631  [identification data]
pI=6.16; Mw=36631  [identification data]
pI=6.16; Mw=36631  [identification data]

IDENTIFICATION: SPOT 348: SeqCov=37.7%. Peptides MS=12/20. Mascot PMF Score=103 [1]
SPOT 440: SeqCov=27.2%. Peptides MS=9/20. Mascot PMF Score=63 [1]; SPOT 513: SeqCov=32%. Peptides MS=10/20. Mascot PMF Score=77 [1]; SPOT 514: SeqCov=40.1%. Peptides MS=14/20. Mascot PMF Score=122 [1].
PEPTIDE EVIDENCE: SPOT 348: 221-GEFITTVQQR-230 (68) / 299-FVEGLPINDFSR-310 (77) / 180-NVIIWGNHSSTQYPDVNHAK-199 (95) [1]
SPOT 440: 221-GEFITTVQQR-230 (62) / 299-FVEGLPINDFSR-310 (88) [1]; SPOT 513: 221-GEFITTVQQR-230 (49) / 299-FVEGLPINDFSR-310 (69) [1].
MAPPING (identification):
SPOT 348: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 440: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 513: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 514: Peptide mass fingerprinting [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtO88989; MDHC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameMDHC_RAT
Primary accession numberO88989
Secondary accession number(s) O88585 Q6PCV2
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 84)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene nameName=Mdh1
Synonyms=Mdh
Encoded onName=Mdh1; Synonyms=Mdh
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF093773; AAC64180.1; -; mRNA
EMBLBC059124; AAH59124.1; -; mRNA
EMBLAF075574; AAC26799.1; -; mRNA
IPIIPI00198717; -; .
RefSeqNP_150238.1; NM_033235.1; .
UniGeneRn.13492; -; .
ProteinModelPortalO88989; -; .
SMRO88989; 2-334; .
IntActO88989; 1; .
STRINGO88989; -; .
PhosphoSiteO88989; -; .
World-2DPAGE0004:O88989; -; .
PRIDEO88989; -; .
EnsemblENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID24551; -; .
KEGGrno:24551; -; .
NMPDRfig|10116.3.peg.10844; -; .
UCSCNM_033235; rat; .
CTD4190; -; .
RGD3072; Mdh1; .
eggNOGroNOG11216; -; .
GeneTreeENSGT00530000063410; -; .
HOVERGENHBG006340; -; .
InParanoidO88989; -; .
OrthoDBEOG4CVG78; -; .
PhylomeDBO88989; -; .
NextBio603654; -; .
ArrayExpressO88989; -; .
GenevestigatorO88989; -; .
GermOnlineENSRNOG00000008103; Rattus norvegicus; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GOGO:0051287; F:NAD binding; IDA:RGD; .
GOGO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GOGO:0006108; P:malate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR001252; Malate_DH_AS; .
InterProIPR011274; Malate_DH_NAD-dep_euk; .
InterProIPR010945; Malate_DH_type2; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00025; -; .
PANTHERPTHR23382; MDH_SF1; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01759; MalateDH-SF1; 1; .
TIGRFAMsTIGR01758; MDH_euk_cyt; 1; .
PROSITEPS00068; MDH; 1; .



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server