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Searching in 'World-2DPAGE Repository [0022]' for entry matching: ODBA_RAT




World-2DPAGE Repository (0022):  ODBA_RAT


ODBA_RAT


General information about the entry
View entry in simple text format
Entry nameODBA_RAT
Primary accession numberP11960
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; EC=1.2.4.4; AltName: Full=Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; Short=BCKDE1A; Short=BCKDH E1-alpha; Flags: Precursor; Fragment;.
Gene nameName=Bckdha
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=7.68; Mw=50418  [identification data]
pI=7.68; Mw=50418  [identification data]
pI=7.68; Mw=50418  [identification data]

IDENTIFICATION: SPOT 427: SeqCov=28.6%. Peptides MS=11/20. Mascot PMF Score=97 [1]
SPOT 639: SeqCov=26.3%. Peptides MS=9/20. Mascot PMF Score=76 [1]; SPOT 796: SeqCov=32.7%. Peptides MS=11/20. Mascot PMF Score=93 [1].
PEPTIDE EVIDENCE: SPOT 427: 313-AVAENQPFLIEAMTYR-328 (70) [1]
SPOT 639: 147-TDLVFGQYR-155 (55) / 313-AVAENQPFLIEAMTYR-328 (36) / 426-HLQTYGEHYPLDHFDK-441 (95) [1]; SPOT 796: 147-TDLVFGQYR-155 (40) / 313-AVAENQPFLIEAMTYR-328 (27) / 426-HLQTYGEHYPLDHFDK-441 (117) [1].
MAPPING (identification):
SPOT 427: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 639: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 796: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP11960; ODBA_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameODBA_RAT
Primary accession numberP11960
Sequence was last modified on October 1, 1989 (version 1)
Annotations were last modified on November 16, 2011 (version 106)
Name and origin of the protein
DescriptionRecName: Full=2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; EC=1.2.4.4; AltName: Full=Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; Short=BCKDE1A; Short=BCKDH E1-alpha; Flags: Precursor; Fragment;
Gene nameName=Bckdha
Encoded onName=Bckdha
KeywordsComplete proteome; Metal-binding; Mitochondrion; Oxidoreductase; Phosphoprotein; Potassium; Reference proteome; Thiamine pyrophosphate; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ02827; AAA40811.1; -; mRNA
IPIIPI00365663; -; .
PIRA29468; DERTXA; .
RefSeqNP_036914.1; NM_012782.1; .
UniGeneRn.49145; -; .
ProteinModelPortalP11960; -; .
SMRP11960; 47-441; .
IntActP11960; 1; .
STRINGP11960; -; .
PhosphoSiteP11960; -; .
PRIDEP11960; -; .
GeneID25244; -; .
KEGGrno:25244; -; .
NMPDRfig|10116.3.peg.1316; -; .
UCSCNM_012782; rat; .
CTD593; -; .
RGD2196; Bckdha; .
eggNOGroNOG08618; -; .
HOVERGENHBG002459; -; .
InParanoidP11960; -; .
OrthoDBEOG4RR6JR; -; .
PhylomeDBP11960; -; .
NextBio605839; -; .
ArrayExpressP11960; -; .
GenevestigatorP11960; -; .
GermOnlineENSRNOG00000020607; Rattus norvegicus; .
GOGO:0005947; C:mitochondrial alpha-ketoglutarate dehydrogenase complex; ISS:HGNC; .
GOGO:0047101; F:2-oxoisovalerate dehydrogenase (acylating) activity; TAS:RGD; .
GOGO:0003863; F:3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity; IEA:EC; .
GOGO:0003826; F:alpha-ketoacid dehydrogenase activity; ISS:HGNC; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0032403; F:protein complex binding; IPI:RGD; .
GOGO:0009083; P:branched chain family amino acid catabolic process; ISS:HGNC; .
GOGO:0051591; P:response to cAMP; IEP:RGD; .
GOGO:0051384; P:response to glucocorticoid stimulus; IEP:RGD; .
GOGO:0007584; P:response to nutrient; IEP:RGD; .
InterProIPR001017; DH_E1; .
KOK00166; -; .
PfamPF00676; E1_dh; 1; .



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