World-2DPAGE Repository

Entry name

ODPA_RAT

Primary accession number

P26284

integrated into World-2DPAGE Repository (0004) on

October 13, 2008 (release 1)

2D Annotations were last modified on

May 18, 2011 (version 2)

General Annotations were last modified on

December 22, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC=1.2.4.1; AltName: Full=PDHE1-A type I; Flags: Precursor;.

Gene name

Name=Pdha1

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 406:

pI=6.90; Mw=46000

MAPPING (identification):

SPOT 406: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

P26284; ODPA_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	ODPA_RAT
Primary accession number	P26284
Sequence was last modified on	December 12, 2006 (version 2)
Annotations were last modified on	September 21, 2011 (version 93)
Name and origin of the protein
Description	RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC=1.2.4.1; AltName: Full=PDHE1-A type I; Flags: Precursor;
Gene name	Name=Pdha1
Encoded on	Name=Pdha1
Keywords	Acetylation; Complete proteome; Direct protein sequencing; Glycolysis; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate; Reference proteome; Thiamine pyrophosphate; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	Z12158; CAA78146.1; -; mRNA
IPI	IPI00191707; -; .
PIR	S15891; DERTPA; .
PIR	S21553; DERTP1; .
UniGene	Rn.3655; -; .
ProteinModelPortal	P26284; -; .
SMR	P26284; 29-390; .
STRING	P26284; -; .
PhosphoSite	P26284; -; .
World-2DPAGE	0004:P26284; -; .
PRIDE	P26284; -; .
UCSC	BC098897; rat; .
RGD	3286; Pdha1; .
eggNOG	roNOG13286; -; .
HOVERGEN	HBG001863; -; .
InParanoid	P26284; -; .
OrthoDB	EOG4W0XD6; -; .
PhylomeDB	P26284; -; .
ArrayExpress	P26284; -; .
Genevestigator	P26284; -; .
GermOnline	ENSRNOG00000025383; Rattus norvegicus; .
GO	GO:0005967; C:mitochondrial pyruvate dehydrogenase complex; TAS:RGD; .
GO	GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IDA:RGD; .
GO	GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterPro	IPR001017; DH_E1; .
InterPro	IPR017597; Pyrv_DH_E1_asu_subgrp-y; .
Pfam	PF00676; E1_dh; 1; .
TIGRFAMs	TIGR03182; PDH_E1_alph_y; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: ODPA_RAT

World-2DPAGE Repository (0014): ODPA_RAT

ODPA_RAT

General information about the entry

Entry name

ODPA_RAT

Primary accession number

P26284

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC=1.2.4.1; AltName: Full=PDHE1-A type I; Flags: Precursor;.

Gene name

Name=Pdha1

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2932:

pI=6.99; Mw=45000

IDENTIFICATION: SPOT 2932: SeqCov=42%. MascotPMF=143 [1].
MAPPING (identification):

SPOT 2932: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

P26284; ODPA_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	ODPA_RAT
Primary accession number	P26284
Sequence was last modified on	December 12, 2006 (version 2)
Annotations were last modified on	September 21, 2011 (version 93)
Name and origin of the protein
Description	RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC=1.2.4.1; AltName: Full=PDHE1-A type I; Flags: Precursor;
Gene name	Name=Pdha1
Encoded on	Name=Pdha1
Keywords	Acetylation; Complete proteome; Direct protein sequencing; Glycolysis; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate; Reference proteome; Thiamine pyrophosphate; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	Z12158; CAA78146.1; -; mRNA
IPI	IPI00191707; -; .
PIR	S15891; DERTPA; .
PIR	S21553; DERTP1; .
UniGene	Rn.3655; -; .
ProteinModelPortal	P26284; -; .
SMR	P26284; 29-390; .
STRING	P26284; -; .
PhosphoSite	P26284; -; .
World-2DPAGE	0004:P26284; -; .
PRIDE	P26284; -; .
UCSC	BC098897; rat; .
RGD	3286; Pdha1; .
eggNOG	roNOG13286; -; .
HOVERGEN	HBG001863; -; .
InParanoid	P26284; -; .
OrthoDB	EOG4W0XD6; -; .
PhylomeDB	P26284; -; .
ArrayExpress	P26284; -; .
Genevestigator	P26284; -; .
GermOnline	ENSRNOG00000025383; Rattus norvegicus; .
GO	GO:0005967; C:mitochondrial pyruvate dehydrogenase complex; TAS:RGD; .
GO	GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IDA:RGD; .
GO	GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterPro	IPR001017; DH_E1; .
InterPro	IPR017597; Pyrv_DH_E1_asu_subgrp-y; .
Pfam	PF00676; E1_dh; 1; .
TIGRFAMs	TIGR03182; PDH_E1_alph_y; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: ODPA_RAT

World-2DPAGE Repository (0019): ODPA_RAT

ODPA_RAT

General information about the entry

Entry name

ODPA_RAT

Primary accession number

P26284

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC=1.2.4.1; AltName: Full=PDHE1-A type I; Flags: Precursor;.

Gene name

Name=Pdha1

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 507:

pI=8.35; Mw=43852

SPOT 513:

pI=8.35; Mw=43852

IDENTIFICATION: SPOT 507: Peptides number=6. SeqCov=10.3%. MOWSE score=1.47E+5 [1]
SPOT 513: Peptides number=3. SeqCov=6.7%. MOWSE score=1.95E+2 [1].
MAPPING (identification):

SPOT 507: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 513: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

P26284; ODPA_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	ODPA_RAT
Primary accession number	P26284
Sequence was last modified on	December 12, 2006 (version 2)
Annotations were last modified on	September 21, 2011 (version 93)
Name and origin of the protein
Description	RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC=1.2.4.1; AltName: Full=PDHE1-A type I; Flags: Precursor;
Gene name	Name=Pdha1
Encoded on	Name=Pdha1
Keywords	Acetylation; Complete proteome; Direct protein sequencing; Glycolysis; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate; Reference proteome; Thiamine pyrophosphate; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	Z12158; CAA78146.1; -; mRNA
IPI	IPI00191707; -; .
PIR	S15891; DERTPA; .
PIR	S21553; DERTP1; .
UniGene	Rn.3655; -; .
ProteinModelPortal	P26284; -; .
SMR	P26284; 29-390; .
STRING	P26284; -; .
PhosphoSite	P26284; -; .
World-2DPAGE	0004:P26284; -; .
PRIDE	P26284; -; .
UCSC	BC098897; rat; .
RGD	3286; Pdha1; .
eggNOG	roNOG13286; -; .
HOVERGEN	HBG001863; -; .
InParanoid	P26284; -; .
OrthoDB	EOG4W0XD6; -; .
PhylomeDB	P26284; -; .
ArrayExpress	P26284; -; .
Genevestigator	P26284; -; .
GermOnline	ENSRNOG00000025383; Rattus norvegicus; .
GO	GO:0005967; C:mitochondrial pyruvate dehydrogenase complex; TAS:RGD; .
GO	GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IDA:RGD; .
GO	GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterPro	IPR001017; DH_E1; .
InterPro	IPR017597; Pyrv_DH_E1_asu_subgrp-y; .
Pfam	PF00676; E1_dh; 1; .
TIGRFAMs	TIGR03182; PDH_E1_alph_y; 1; .

Searching in 'World-2DPAGE Repository [0069]' for entry matching: ODPA_RAT

World-2DPAGE Repository (0069): ODPA_RAT

ODPA_RAT

General information about the entry

Entry name

ODPA_RAT

Primary accession number

ODPA_RAT

integrated into World-2DPAGE Repository (0069) on

February 27, 2014 (release 1)

2D Annotations were last modified on

February 27, 2014 (version 1)

General Annotations were last modified on

February 27, 2014 (version 1)

Name and origin of the protein

Description

ODPA_RAT.

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D GEL CHARACTERIZATION
DOI=10.1016/j.jprot.2014.04.015;
Burniston J.G., Kenyani J., Gray D., Guadagnin E., Jarman I.H., Cobley J.N., Cuthbertson D.J., Chen Y.-W., Wastling J.M., Lisboa P., Koch L.G., Britton S.L.
''Conditional independence mapping of DIGE data reveals PDIA3 protein species as key nodes associated with muscle aerobic capacity''
Journal of Proteomics 106(1):230-245 (2014)

2D PAGE maps for identified proteins

RATTUS_SOLEUS-DIGE_4.5-9.5 {M+F HCR LCR Soleus DiGE Exp}
Rattus
Tissue: Skeletal muscle