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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P02650

World-2DPAGE Repository (0022):  P02650


General information about the entry
View entry in simple text format
Entry nameAPOE_RAT
Primary accession numberP02650
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Apolipoprotein E; Short=Apo-E; Flags: Precursor;.
Gene nameName=Apoe
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

Tissue: Heart
  map experimental info

pI=5.23; Mw=35788  [identification data]

IDENTIFICATION: SPOT 89: SeqCov=5.23%. Peptides MS=12/20. Mascot PMF Score=98 [1].
PEPTIDE EVIDENCE: SPOT 89: 43-FWDYLR-48 (34) / 262-LQAEIFQAR-270 (61) / 273-GWFEPLVEDMQR-284 (46) [1].
MAPPING (identification):
SPOT 89: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
UniProtKB/Swiss-ProtP02650; APOE_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameAPOE_RAT
Primary accession numberP02650
Sequence was last modified on October 1, 1996 (version 2)
Annotations were last modified on November 16, 2011 (version 100)
Name and origin of the protein
DescriptionRecName: Full=Apolipoprotein E; Short=Apo-E; Flags: Precursor;
Gene nameName=Apoe
Encoded onName=Apoe
KeywordsChylomicron; Complete proteome; Direct protein sequencing; HDL; Heparin-binding; Lipid transport; Phosphoprotein; Reference proteome; Repeat; Secreted; Signal; Transport; VLDL.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLX04979; CAA28650.1; -; Genomic_DNA
EMBLJ02582; AAA40755.1; -; Genomic_DNA
EMBLS76779; AAC60703.1; -; mRNA
EMBLBC086581; AAH86581.1; -; mRNA
IPIIPI00190701; -; .
PIRA26189; LPRTE; .
RefSeqNP_620183.2; NM_138828.2; .
UniGeneRn.32351; -; .
ProteinModelPortalP02650; -; .
SMRP02650; 23-195; .
IntActP02650; 2; .
STRINGP02650; -; .
PRIDEP02650; -; .
EnsemblENSRNOT00000041891; ENSRNOP00000050968; ENSRNOG00000018454; .
GeneID25728; -; .
KEGGrno:25728; -; .
UCSCBC086581; rat; .
CTD348; -; .
RGD2138; Apoe; .
eggNOGroNOG10307; -; .
HOVERGENHBG010582; -; .
InParanoidP02650; -; .
OrthoDBEOG4GB76S; -; .
PhylomeDBP02650; -; .
NextBio607845; -; .
ArrayExpressP02650; -; .
GenevestigatorP02650; -; .
GermOnlineENSRNOG00000018454; Rattus norvegicus; .
GOGO:0042627; C:chylomicron; IEA:UniProtKB-KW; .
GOGO:0030425; C:dendrite; IDA:RGD; .
GOGO:0031232; C:extrinsic to external side of plasma membrane; IDA:RGD; .
GOGO:0005794; C:Golgi apparatus; IDA:RGD; .
GOGO:0034364; C:high-density lipoprotein particle; IEA:UniProtKB-KW; .
GOGO:0005770; C:late endosome; IDA:RGD; .
GOGO:0043025; C:neuronal cell body; IDA:RGD; .
GOGO:0034361; C:very-low-density lipoprotein particle; IEA:UniProtKB-KW; .
GOGO:0001540; F:beta-amyloid binding; ISS:UniProtKB; .
GOGO:0008201; F:heparin binding; IEA:UniProtKB-KW; .
GOGO:0005543; F:phospholipid binding; IDA:RGD; .
GOGO:0007568; P:aging; IDA:RGD; .
GOGO:0071363; P:cellular response to growth factor stimulus; IEP:RGD; .
GOGO:0006917; P:induction of apoptosis; ISS:UniProtKB; .
GOGO:0014012; P:peripheral nervous system axon regeneration; IEP:RGD; .
GOGO:0045471; P:response to ethanol; IDA:RGD; .
InterProIPR013326; ApoA/E_ApoLp; .
InterProIPR000074; ApoA1_A4_E; .
Gene3DG3DSA:; ApoA/E_ApoLp; 2; .
KOK04524; -; .
PfamPF01442; Apolipoprotein; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server