resource logo

World-2DPAGE Repository

Attention: World-2DPAGE is no longer maintained.

World-2DPAGE Repository no longer accepts submissions.


World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Databases

use 'Ctrl' to select several
databases


Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal

Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0022]' for entry matching: P04764




World-2DPAGE Repository (0022):  P04764


P04764


General information about the entry
View entry in simple text format
Entry nameENOA_RAT
Primary accession numberP04764
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Alpha-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 1; AltName: Full=Non-neural enolase; Short=NNE;.
Gene nameName=Eno1
Synonyms=Eno-1
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.16; Mw=47440  [identification data]
pI=6.16; Mw=47440  [identification data]
pI=6.16; Mw=47440  [identification data]
pI=6.16; Mw=47440  [identification data]
pI=6.16; Mw=47440  [identification data]
pI=6.16; Mw=47440  [identification data]

IDENTIFICATION: SPOT 272: SeqCov=21.9%. Peptides MS=11/20. Mascot PMF Score=67 [1]
SPOT 306: SeqCov=25.1%. Peptides MS=11/20. Mascot PMF Score=79 [1]; SPOT 353: SeqCov=31.3%. Peptides MS=13/20. Mascot PMF Score=99 [1]; SPOT 426: SeqCov=15%. Peptides MS=7/20. Mascot PMF Score=40 [1]; SPOT 470: SeqCov=26.7%. Peptides MS=11/20. Mascot PMF Score=72 [1]; SPOT 799: SeqCov=30.9%. Peptides MS=13/20. Mascot PMF Score=98 [1].
PEPTIDE EVIDENCE: SPOT 272: 407-YNQILR-412 (34) / 33-AAVPSGASTGIYEALELR-50 (101) / 234-AGYTDQVVIGMDVAASEFYR-253 (123) [1]
SPOT 306: 127-GVPLYR-132 (19) / 407-YNQILR-412 (9) / 33-AAVPSGASTGIYEALELR-50 (60) [1]; SPOT 353: 33-AAVPSGASTGIYEALELR-50 (125) / 163-LAMQEFMILPVGASSFR-179 (76) / 234-AGYTDQVVIGMDVAASEFYR-253 (146) [1]; SPOT 426: 33-AAVPSGASTGIYEALELR-50 (56) [1]; SPOT 470: 407-YNQILR-412 (19) / 163-LAMQEFMILPVGASSFR-179 (69) / 234-AGYTDQVVIGMDVAASEFYR-253 (136) [1]; SPOT 799: 33-AAVPSGASTGIYEALELR-50 (102) / 163-LAMQEFMILPVGASSFR-179 (63) / 234-AGYTDQVVIGMDVAASEFYR-253 (136) [1].
MAPPING (identification):
SPOT 272: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 306: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 353: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 426: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 470: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 799: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP04764; ENOA_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameENOA_RAT
Primary accession numberP04764
Secondary accession number(s) Q66HI3 Q6AYV3 Q6P504
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on November 16, 2011 (version 122)
Name and origin of the protein
DescriptionRecName: Full=Alpha-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 1; AltName: Full=Non-neural enolase; Short=NNE;
Gene nameName=Eno1
Synonyms=Eno-1
Encoded onName=Eno1; Synonyms=Eno-1
KeywordsAcetylation; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Phosphoprotein; Plasminogen activation; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX02610; CAA26456.1; -; mRNA
EMBLBC063174; AAH63174.1; ALT_INIT; mRNA
EMBLBC078896; AAH78896.1; -; mRNA
EMBLBC081847; AAH81847.2; -; mRNA
EMBLAF241613; AAK01319.1; -; mRNA
IPIIPI00464815; -; .
PIRA23126; A23126; .
RefSeqNP_001103378.1; NM_001109908.1; .
RefSeqNP_036686.2; NM_012554.2; .
UniGeneRn.4236; -; .
ProteinModelPortalP04764; -; .
SMRP04764; 2-434; .
IntActP04764; 2; .
STRINGP04764; -; .
PhosphoSiteP04764; -; .
World-2DPAGE0004:P04764; -; .
PRIDEP04764; -; .
EnsemblENSRNOT00000024106; ENSRNOP00000024106; ENSRNOG00000017895; .
GeneID24333; -; .
KEGGrno:24333; -; .
UCSCNM_012554; rat; .
CTD2023; -; .
RGD2553; Eno1; .
eggNOGroNOG10078; -; .
HOVERGENHBG000067; -; .
InParanoidP04764; -; .
OMAKVEIGMD; -; .
OrthoDBEOG4T783B; -; .
PhylomeDBP04764; -; .
NextBio603015; -; .
GenevestigatorP04764; -; .
GermOnlineENSRNOG00000017895; Rattus norvegicus; .
GOGO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0019717; C:synaptosome; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0004634; F:phosphopyruvate hydratase activity; IDA:RGD; .
GOGO:0046982; F:protein heterodimerization activity; IDA:RGD; .
GOGO:0042803; F:protein homodimerization activity; IDA:RGD; .
GOGO:0006096; P:glycolysis; TAS:RGD; .
InterProIPR000941; Enolase; .
InterProIPR020810; Enolase_C; .
InterProIPR020809; Enolase_CS; .
InterProIPR020811; Enolase_N; .
KOK01689; -; .
PANTHERPTHR11902; Enolase; 1; .
PfamPF00113; Enolase_C; 1; .
PfamPF03952; Enolase_N; 1; .
PIRSFPIRSF001400; Enolase; 1; .
PRINTSPR00148; ENOLASE; .
TIGRFAMsTIGR01060; Eno; 1; .
PROSITEPS00164; ENOLASE; 1; .



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server