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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P04764




World-2DPAGE Repository (0030):  P04764


P04764


General information about the entry
View entry in simple text format
Entry nameENOA_RAT
Primary accession numberP04764
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Alpha-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 1; AltName: Full=Non-neural enolase; Short=NNE;.
Gene nameName=Eno1
Synonyms=Eno-1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=4.44; Mw=60727  [identification data]
pI=5.85; Mw=59070  [identification data]
pI=6.30; Mw=58582  [identification data]
pI=4.95; Mw=56512  [identification data]
pI=5.72; Mw=56512  [identification data]
pI=6.63; Mw=55967  [identification data]
pI=5.82; Mw=58907  [identification data]

IDENTIFICATION: SPOT 1066: Mascot protein score=249. Number of peptides=9 [1]
SPOT 1106: Mascot protein score=135. Number of peptides=6 [1]; SPOT 1116: Mascot protein score=468. Number of peptides=7 [1]; SPOT 1182: Mascot protein score=106. Number of peptides=4 [1]; SPOT 1183: Mascot protein score=62. Number of peptides=5 [1]; SPOT 1193: Mascot protein score=288. Number of peptides=7 [1]; SPOT 2996: Mascot protein score=128. Number of peptides=8 [1].
MAPPING (identification):
SPOT 1066: Tandem mass spectrometry [1];
SPOT 1106: Tandem mass spectrometry [1];
SPOT 1116: Tandem mass spectrometry [1];
SPOT 1182: Tandem mass spectrometry [1];
SPOT 1183: Tandem mass spectrometry [1];
SPOT 1193: Tandem mass spectrometry [1];
SPOT 2996: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP04764; ENOA_RAT.
World-2DPAGE RepositoryP04764; ENOA_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameENOA_RAT
Primary accession numberP04764
Secondary accession number(s) Q66HI3 Q6AYV3 Q6P504
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on November 16, 2011 (version 122)
Name and origin of the protein
DescriptionRecName: Full=Alpha-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 1; AltName: Full=Non-neural enolase; Short=NNE;
Gene nameName=Eno1
Synonyms=Eno-1
Encoded onName=Eno1; Synonyms=Eno-1
KeywordsAcetylation; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Phosphoprotein; Plasminogen activation; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX02610; CAA26456.1; -; mRNA
EMBLBC063174; AAH63174.1; ALT_INIT; mRNA
EMBLBC078896; AAH78896.1; -; mRNA
EMBLBC081847; AAH81847.2; -; mRNA
EMBLAF241613; AAK01319.1; -; mRNA
IPIIPI00464815; -; .
PIRA23126; A23126; .
RefSeqNP_001103378.1; NM_001109908.1; .
RefSeqNP_036686.2; NM_012554.2; .
UniGeneRn.4236; -; .
ProteinModelPortalP04764; -; .
SMRP04764; 2-434; .
IntActP04764; 2; .
STRINGP04764; -; .
PhosphoSiteP04764; -; .
World-2DPAGE0004:P04764; -; .
PRIDEP04764; -; .
EnsemblENSRNOT00000024106; ENSRNOP00000024106; ENSRNOG00000017895; .
GeneID24333; -; .
KEGGrno:24333; -; .
UCSCNM_012554; rat; .
CTD2023; -; .
RGD2553; Eno1; .
eggNOGroNOG10078; -; .
HOVERGENHBG000067; -; .
InParanoidP04764; -; .
OMAKVEIGMD; -; .
OrthoDBEOG4T783B; -; .
PhylomeDBP04764; -; .
NextBio603015; -; .
GenevestigatorP04764; -; .
GermOnlineENSRNOG00000017895; Rattus norvegicus; .
GOGO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0019717; C:synaptosome; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0004634; F:phosphopyruvate hydratase activity; IDA:RGD; .
GOGO:0046982; F:protein heterodimerization activity; IDA:RGD; .
GOGO:0042803; F:protein homodimerization activity; IDA:RGD; .
GOGO:0006096; P:glycolysis; TAS:RGD; .
InterProIPR000941; Enolase; .
InterProIPR020810; Enolase_C; .
InterProIPR020809; Enolase_CS; .
InterProIPR020811; Enolase_N; .
KOK01689; -; .
PANTHERPTHR11902; Enolase; 1; .
PfamPF00113; Enolase_C; 1; .
PfamPF03952; Enolase_N; 1; .
PIRSFPIRSF001400; Enolase; 1; .
PRINTSPR00148; ENOLASE; .
TIGRFAMsTIGR01060; Eno; 1; .
PROSITEPS00164; ENOLASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server