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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P06761




World-2DPAGE Repository (0022):  P06761


P06761


General information about the entry
View entry in simple text format
Entry nameGRP78_RAT
Primary accession numberP06761
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName: Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy chain-binding protein; Short=BiP; AltName: Full=Steroidogenesis-activator polypeptide; Flags: Precursor;.
Gene nameName=Hspa5
Synonyms=Grp78
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=5.07; Mw=72473  [identification data]
pI=5.07; Mw=72473  [identification data]
pI=5.07; Mw=72473  [identification data]

IDENTIFICATION: SPOT 414: SeqCov=21.3%. Peptides MS=10/20. Mascot PMF Score=78 [1]
SPOT 423: SeqCov=19.7%. Peptides MS=9/20. Mascot PMF Score=67 [1]; SPOT 578: SeqCov=12.1%. Peptides MS=5/20. Mascot PMF Score=26 [1].
PEPTIDE EVIDENCE: SPOT 414: 353-KSDIDEIVLVGGSTR-367 (98) / 475-DNHLLGTFDLTGIPPAPR-492 (116) / 307-IEIESFFEGEDFSETLTR-324 (125) [1]
SPOT 423: 353-KSDIDEIVLVGGSTR-367 (75): 475-DNHLLGTFDLTGIPPAPR-492 (90): 307-IEIESFFEGEDFSETLTR-324 (120) [1]; SPOT 578: 61-ITPSYVAFTPEGER-74 (30) [1].
MAPPING (identification):
SPOT 414: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 423: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 578: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP06761; GRP78_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameGRP78_RAT
Primary accession numberP06761
Sequence was last modified on April 1, 1988 (version 1)
Annotations were last modified on November 16, 2011 (version 117)
Name and origin of the protein
DescriptionRecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName: Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy chain-binding protein; Short=BiP; AltName: Full=Steroidogenesis-activator polypeptide; Flags: Precursor;
Gene nameName=Hspa5
Synonyms=Grp78
Encoded onName=Hspa5; Synonyms=Grp78
KeywordsAcetylation; ATP-binding; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Nucleotide-binding; Phosphoprotein; Reference proteome; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM14050; AAA40817.1; -; mRNA
EMBLBC062017; AAH62017.1; -; mRNA
EMBLM14866; AAA41277.1; -; Genomic_DNA
IPIIPI00206624; -; .
PIRA23948; HHRTGB; .
RefSeqNP_037215.1; NM_013083.2; .
UniGeneRn.11088; -; .
ProteinModelPortalP06761; -; .
SMRP06761; 28-570; .
IntActP06761; 5; .
MINTMINT-4575479; -; .
STRINGP06761; -; .
PhosphoSiteP06761; -; .
PRIDEP06761; -; .
EnsemblENSRNOT00000025067; ENSRNOP00000025064; ENSRNOG00000018294; .
GeneID25617; -; .
KEGGrno:25617; -; .
NMPDRfig|10116.3.peg.18461; -; .
UCSCNM_013083; rat; .
CTD3309; -; .
RGD2843; Hspa5; .
eggNOGmaNOG11047; -; .
GeneTreeENSGT00600000084521; -; .
HOVERGENHBG051845; -; .
InParanoidP06761; -; .
OMANQLTTNP; -; .
OrthoDBEOG444KJV; -; .
PhylomeDBP06761; -; .
NextBio607375; -; .
ArrayExpressP06761; -; .
GenevestigatorP06761; -; .
GermOnlineENSRNOG00000018294; Rattus norvegicus; .
GOGO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; IPI:RGD; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
KOK09490; -; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
PROSITEPS00014; ER_TARGET; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server