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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P07323




World-2DPAGE Repository (0030):  P07323


P07323


General information about the entry
View entry in simple text format
Entry nameENOG_RAT
Primary accession numberP07323
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Gamma-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 2; AltName: Full=Neural enolase; AltName: Full=Neuron-specific enolase; Short=NSE;.
Gene nameName=Eno2
Synonyms=Eno-2
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=4.44; Mw=60727  [identification data]
pI=5.91; Mw=58339  [identification data]

IDENTIFICATION: SPOT 1066: Mascot protein score=249. Number of peptides=9 [1]
SPOT 1137: Mascot protein score=229. Number of peptides=9 [1].
MAPPING (identification):
SPOT 1066: Tandem mass spectrometry [1];
SPOT 1137: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP07323; ENOG_RAT.
World-2DPAGE RepositoryP07323; ENOG_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameENOG_RAT
Primary accession numberP07323
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 120)
Name and origin of the protein
DescriptionRecName: Full=Gamma-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 2; AltName: Full=Neural enolase; AltName: Full=Neuron-specific enolase; Short=NSE;
Gene nameName=Eno2
Synonyms=Eno-2
Encoded onName=Eno2; Synonyms=Eno-2
KeywordsCell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM11931; AAA41119.1; -; mRNA
EMBLM22770; AAA41725.1; -; Genomic_DNA
EMBLAF019973; AAB72088.1; -; mRNA
EMBLBC060310; AAH60310.1; -; mRNA
EMBLX07727; CAA30556.1; -; Genomic_DNA
EMBLX07728; CAA30556.1; JOINED; Genomic_DNA
EMBLX07729; CAA30556.1; JOINED; Genomic_DNA
IPIIPI00326412; -; .
PIRA24742; A24742; .
PIRJC1039; JC1039; .
RefSeqNP_647541.1; NM_139325.2; .
UniGeneRn.10828; -; .
ProteinModelPortalP07323; -; .
SMRP07323; 2-434; .
STRINGP07323; -; .
PhosphoSiteP07323; -; .
World-2DPAGE0004:P07323; -; .
PRIDEP07323; -; .
EnsemblENSRNOT00000005601; ENSRNOP00000005601; ENSRNOG00000013141; .
GeneID24334; -; .
KEGGrno:24334; -; .
NMPDRfig|10116.3.peg.22203; -; .
UCSCNM_139325; rat; .
CTD2026; -; .
RGD2554; Eno2; .
eggNOGroNOG10078; -; .
GeneTreeENSGT00550000074560; -; .
HOVERGENHBG000067; -; .
InParanoidP07323; -; .
OMAQAVDHIN; -; .
OrthoDBEOG4T783B; -; .
PhylomeDBP07323; -; .
NextBio603019; -; .
ArrayExpressP07323; -; .
GenevestigatorP07323; -; .
GermOnlineENSRNOG00000013141; Rattus norvegicus; .
GOGO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0019717; C:synaptosome; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0004634; F:phosphopyruvate hydratase activity; IDA:RGD; .
GOGO:0046982; F:protein heterodimerization activity; IDA:RGD; .
GOGO:0042803; F:protein homodimerization activity; IDA:RGD; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0006096; P:glycolysis; IDA:RGD; .
GOGO:0042493; P:response to drug; IEP:RGD; .
GOGO:0032355; P:response to estradiol stimulus; IEP:RGD; .
GOGO:0014070; P:response to organic cyclic compound; IEP:RGD; .
InterProIPR000941; Enolase; .
InterProIPR020810; Enolase_C; .
InterProIPR020809; Enolase_CS; .
InterProIPR020811; Enolase_N; .
KOK01689; -; .
PANTHERPTHR11902; Enolase; 1; .
PfamPF00113; Enolase_C; 1; .
PfamPF03952; Enolase_N; 1; .
PIRSFPIRSF001400; Enolase; 1; .
PRINTSPR00148; ENOLASE; .
TIGRFAMsTIGR01060; Eno; 1; .
PROSITEPS00164; ENOLASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server