World-2DPAGE Repository

RecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; EC=2.3.1.12; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor;.

Gene name

Name=Dlat

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

map experimental info

OB21DSUB

MAP LOCATIONS:

SPOT 243:

pI=5.62; Mw=63000

[identification data]

MAPPING (identification):

SPOT 243: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

P08461; ODP2_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	ODP2_RAT
Primary accession number	P08461
Secondary accession number(s)	Q3B7V7
Sequence was last modified on	December 12, 2006 (version 3)
Annotations were last modified on	November 16, 2011 (version 119)
Name and origin of the protein
Description	RecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; EC=2.3.1.12; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor;
Gene name	Name=Dlat
Encoded on	Name=Dlat
Keywords	Acetylation; Acyltransferase; Complete proteome; Direct protein sequencing; Glycolysis; Lipoyl; Mitochondrion; Reference proteome; Repeat; Transferase; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC107440; AAI07441.1; -; mRNA
EMBL	D10655; BAA01504.1; -; mRNA
EMBL	D00092; BAA20956.1; -; mRNA
EMBL	M16075; AAA41813.1; -; mRNA
IPI	IPI00231714; -; .
PIR	S21766; S21766; .
RefSeq	NP_112287.1; NM_031025.1; .
UniGene	Rn.15413; -; .
ProteinModelPortal	P08461; -; .
SMR	P08461; 83-173; 204-305; 394-632
IntAct	P08461; 1; .
STRING	P08461; -; .
PhosphoSite	P08461; -; .
World-2DPAGE	0004:P08461; -; .
PRIDE	P08461; -; .
Ensembl	ENSRNOT00000032152; ENSRNOP00000032890; ENSRNOG00000009994; .
GeneID	81654; -; .
KEGG	rno:81654; -; .
NMPDR	fig\|10116.3.peg.28575; -; .
UCSC	NM_031025; rat; .
CTD	1737; -; .
RGD	619859; Dlat; .
eggNOG	roNOG14419; -; .
GeneTree	ENSGT00560000077144; -; .
HOVERGEN	HBG005063; -; .
InParanoid	P08461; -; .
OMA	YASPMAK; -; .
OrthoDB	EOG412M54; -; .
PhylomeDB	P08461; -; .
NextBio	615200; -; .
ArrayExpress	P08461; -; .
Genevestigator	P08461; -; .
GermOnline	ENSRNOG00000009994; Rattus norvegicus; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD; .
GO	GO:0004742; F:dihydrolipoyllysine-residue acetyltransferase activity; IDA:RGD; .
GO	GO:0000166; F:nucleotide binding; TAS:RGD; .
GO	GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
GO	GO:0030431; P:sleep; IEP:RGD; .
InterPro	IPR003016; 2-oxoA_DH_lipoyl-BS; .
InterPro	IPR001078; 2-oxoacid_DH_actylTfrase; .
InterPro	IPR006257; AcTrfase_Pyrv_DH_cplx_L; .
InterPro	IPR000089; Biotin_lipoyl; .
InterPro	IPR023213; CAT-like_dom; .
InterPro	IPR004167; E3-bd; .
InterPro	IPR011053; Single_hybrid_motif; .
Gene3D	G3DSA:3.30.559.10; CAT-like_dom; 1; .
Gene3D	G3DSA:4.10.320.10; E3_bd; 1; .
KO	K00627; -; .
Pfam	PF00198; 2-oxoacid_dh; 1; .
Pfam	PF00364; Biotin_lipoyl; 2; .
Pfam	PF02817; E3_binding; 1; .
SUPFAM	SSF47005; E3_bd; 1; .
SUPFAM	SSF51230; Hybrid_motif; 2; .
TIGRFAMs	TIGR01349; PDHac_trf_mito; 1; .
PROSITE	PS50968; BIOTINYL_LIPOYL; 2; .
PROSITE	PS00189; LIPOYL; 2; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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