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Searching in 'World-2DPAGE Repository [0019]' for entry matching: P08461




World-2DPAGE Repository (0019):  P08461

P08461


General information about the entry
View entry in simple text format
Entry nameODP2_RAT
Primary accession numberP08461
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; EC=2.3.1.12; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor;.
Gene nameName=Dlat
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1111/j.1471-4159.2010.06719.x;
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
HIPP_SYNAP
  map experimental info 		 
HIPP_SYNAP

MAP LOCATIONS:
pI=5.53; Mw=57645  [identification data]
pI=5.53; Mw=57645  [identification data]

IDENTIFICATION: SPOT 1143: Peptides number=5. SeqCov=10.3%. MOWSE score=9.39E+3 [1]
SPOT 1146: Peptides number=4. SeqCov=7.6%. MOWSE score=9.42E+3 [1].
MAPPING (identification):
SPOT 1143: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1146: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Willard Freeman, Penn State College of Medicine, USA
Cross-references
UniProtKB/Swiss-ProtP08461; ODP2_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameODP2_RAT
Primary accession numberP08461
Secondary accession number(s) Q3B7V7
Sequence was last modified on December 12, 2006 (version 3)
Annotations were last modified on November 16, 2011 (version 119)
Name and origin of the protein
DescriptionRecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; EC=2.3.1.12; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor;
Gene nameName=Dlat
Encoded onName=Dlat
KeywordsAcetylation; Acyltransferase; Complete proteome; Direct protein sequencing; Glycolysis; Lipoyl; Mitochondrion; Reference proteome; Repeat; Transferase; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBC107440; AAI07441.1; -; mRNA
EMBLD10655; BAA01504.1; -; mRNA
EMBLD00092; BAA20956.1; -; mRNA
EMBLM16075; AAA41813.1; -; mRNA
IPIIPI00231714; -; .
PIRS21766; S21766; .
RefSeqNP_112287.1; NM_031025.1; .
UniGeneRn.15413; -; .
ProteinModelPortalP08461; -; .
SMRP08461; 83-173; 204-305; 394-632
IntActP08461; 1; .
STRINGP08461; -; .
PhosphoSiteP08461; -; .
World-2DPAGE0004:P08461; -; .
PRIDEP08461; -; .
EnsemblENSRNOT00000032152; ENSRNOP00000032890; ENSRNOG00000009994; .
GeneID81654; -; .
KEGGrno:81654; -; .
NMPDRfig|10116.3.peg.28575; -; .
UCSCNM_031025; rat; .
CTD1737; -; .
RGD619859; Dlat; .
eggNOGroNOG14419; -; .
GeneTreeENSGT00560000077144; -; .
HOVERGENHBG005063; -; .
InParanoidP08461; -; .
OMAYASPMAK; -; .
OrthoDBEOG412M54; -; .
PhylomeDBP08461; -; .
NextBio615200; -; .
ArrayExpressP08461; -; .
GenevestigatorP08461; -; .
GermOnlineENSRNOG00000009994; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD; .
GOGO:0004742; F:dihydrolipoyllysine-residue acetyltransferase activity; IDA:RGD; .
GOGO:0000166; F:nucleotide binding; TAS:RGD; .
GOGO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
GOGO:0030431; P:sleep; IEP:RGD; .
InterProIPR003016; 2-oxoA_DH_lipoyl-BS; .
InterProIPR001078; 2-oxoacid_DH_actylTfrase; .
InterProIPR006257; AcTrfase_Pyrv_DH_cplx_L; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR023213; CAT-like_dom; .
InterProIPR004167; E3-bd; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.30.559.10; CAT-like_dom; 1; .
Gene3DG3DSA:4.10.320.10; E3_bd; 1; .
KOK00627; -; .
PfamPF00198; 2-oxoacid_dh; 1; .
PfamPF00364; Biotin_lipoyl; 2; .
PfamPF02817; E3_binding; 1; .
SUPFAMSSF47005; E3_bd; 1; .
SUPFAMSSF51230; Hybrid_motif; 2; .
TIGRFAMsTIGR01349; PDHac_trf_mito; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 2; .
PROSITEPS00189; LIPOYL; 2; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server