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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0022]' for entry matching: P08461 World-2DPAGE Repository (0022):  P08461 P08461 General information about the entry View entry in simple text format Entry name ODP2_RAT Primary accession number P08461 integrated into World-2DPAGE Repository (0022) on  May 7, 2010 (release 1) 2D Annotations were last modified on June 22, 2011 (version 2) General Annotations were last modified on  November 24, 2011 (version 2) Name and origin of the protein Description RecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; EC=2.3.1.12; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor;. Gene name Name=Dlat Annotated species Rattus [TaxID: 10114] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000593; Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L. ''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity'' Proteomics 11(16):3369-3379 (2011) 2D PAGE maps for identified proteins How to interpret a protein HCR_LCR_HEART {HCR LCR Cardiac Map} Rattus Tissue: Heart   map experimental info   HCR_LCR_HEART MAP LOCATIONS: SPOT 147: pI=8.76; Mw=67637   [identification data] SPOT 172: pI=8.76; Mw=67637   [identification data] SPOT 618: pI=8.76; Mw=67637   [identification data] SPOT 781: pI=8.76; Mw=67637   [identification data] IDENTIFICATION: SPOT 147: SeqCov=20.4%. Peptides MS=10/20. Mascot PMF Score=62 [1] SPOT 172: SeqCov=21.7%. Peptides MS=11/20. Mascot PMF Score=73 [1]; SPOT 618: SeqCov=22.6%. Peptides MS=10/20. Mascot PMF Score=79 [1]; SPOT 781: SeqCov=19.1%. Peptides MS=7/20. Mascot PMF Score=48 [1]. PEPTIDE EVIDENCE: SPOT 147: 139-ILVPEGTR-146 (21) / 397-VAPTPAGVFIDIPISNIR-414 (101) / 426-QTIPHYYLSVDVNMGEVLLVR-446 (21) [1] SPOT 172: 139-ILVPEGTR-146 (34) / 383-AAPAAAAAAPPGPR-396 (43) / 397-VAPTPAGVFIDIPISNIR-414 (78) [1]; SPOT 618: 139-ILVPEGTR-146 (31) / 383-AAPAAAAAAPPGPR-396 (40) / 397-VAPTPAGVFIDIPISNIR-414 (119) [1]; SPOT 781: 397-VAPTPAGVFIDIPISNIR-414 (48) [1]. MAPPING (identification): SPOT 147: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 172: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 618: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 781: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]. Copyright Data from Dr. Jatin Burniston, Liverpool John Moores University, UK Cross-references UniProtKB/Swiss-Prot P08461; ODP2_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name ODP2_RAT Primary accession number P08461 Secondary accession number(s) Q3B7V7 Sequence was last modified on  December 12, 2006 (version 3) Annotations were last modified on  November 16, 2011 (version 119) Name and origin of the protein Description RecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; EC=2.3.1.12; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor; Gene name Name=Dlat Encoded on Name=Dlat Keywords Acetylation; Acyltransferase; Complete proteome; Direct protein sequencing; Glycolysis; Lipoyl; Mitochondrion; Reference proteome; Repeat; Transferase; Transit peptide. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL BC107440; AAI07441.1; -; mRNA EMBL D10655; BAA01504.1; -; mRNA EMBL D00092; BAA20956.1; -; mRNA EMBL M16075; AAA41813.1; -; mRNA IPI IPI00231714; -; . PIR S21766; S21766; . RefSeq NP_112287.1; NM_031025.1; . UniGene Rn.15413; -; . ProteinModelPortal P08461; -; . SMR P08461; 83-173; 204-305; 394-632 IntAct P08461; 1; . STRING P08461; -; . PhosphoSite P08461; -; . World-2DPAGE 0004:P08461; -; . PRIDE P08461; -; . Ensembl ENSRNOT00000032152; ENSRNOP00000032890; ENSRNOG00000009994; . GeneID 81654; -; . KEGG rno:81654; -; . NMPDR fig|10116.3.peg.28575; -; . UCSC NM_031025; rat; . CTD 1737; -; . RGD 619859; Dlat; . eggNOG roNOG14419; -; . GeneTree ENSGT00560000077144; -; . HOVERGEN HBG005063; -; . InParanoid P08461; -; . OMA YASPMAK; -; . OrthoDB EOG412M54; -; . PhylomeDB P08461; -; . NextBio 615200; -; . ArrayExpress P08461; -; . Genevestigator P08461; -; . GermOnline ENSRNOG00000009994; Rattus norvegicus; . GO GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; . GO GO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD; . GO GO:0004742; F:dihydrolipoyllysine-residue acetyltransferase activity; IDA:RGD; . GO GO:0000166; F:nucleotide binding; TAS:RGD; . GO GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; . GO GO:0006096; P:glycolysis; IEA:UniProtKB-KW; . GO GO:0030431; P:sleep; IEP:RGD; . InterPro IPR003016; 2-oxoA_DH_lipoyl-BS; . InterPro IPR001078; 2-oxoacid_DH_actylTfrase; . InterPro IPR006257; AcTrfase_Pyrv_DH_cplx_L; . InterPro IPR000089; Biotin_lipoyl; . InterPro IPR023213; CAT-like_dom; . InterPro IPR004167; E3-bd; . InterPro IPR011053; Single_hybrid_motif; . Gene3D G3DSA:3.30.559.10; CAT-like_dom; 1; . Gene3D G3DSA:4.10.320.10; E3_bd; 1; . KO K00627; -; . Pfam PF00198; 2-oxoacid_dh; 1; . Pfam PF00364; Biotin_lipoyl; 2; . Pfam PF02817; E3_binding; 1; . SUPFAM SSF47005; E3_bd; 1; . SUPFAM SSF51230; Hybrid_motif; 2; . TIGRFAMs TIGR01349; PDHac_trf_mito; 1; . PROSITE PS50968; BIOTINYL_LIPOYL; 2; . PROSITE PS00189; LIPOYL; 2; . World-2DPAGE Repository (search AC)