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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P10719




World-2DPAGE Repository (0022):  P10719


P10719


General information about the entry
View entry in simple text format
Entry nameATPB_RAT
Primary accession numberP10719
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=ATP synthase subunit beta, mitochondrial; EC=3.6.3.14; Flags: Precursor;.
Gene nameName=Atp5b
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=5.19; Mw=56318  [identification data]
pI=5.19; Mw=56318  [identification data]
pI=5.19; Mw=56318  [identification data]
pI=5.19; Mw=56318  [identification data]

IDENTIFICATION: SPOT 331: SeqCov=41.8%. Peptides MS=17/20. Mascot PMF Score=196 [1]
SPOT 507: SeqCov=40.1%. Peptides MS=17/20. Mascot PMF Score=184 [1]; SPOT 795: SeqCov=28%. Peptides MS=12/20. Mascot PMF Score=102 [1]; SPOT 875: SeqCov=23.8%. Peptides MS=13/20. Mascot PMF Score=82 [1].
PEPTIDE EVIDENCE: SPOT 795: 226-AHGGYSVFAGVGER-239 (89) / 282-VALTGLTVAEYFR-294 (52) / 295-DQEGQDVLLFIDNIFR-310 (29) [1]
SPOT 875: 226-AHGGYSVFAGVGER-239 (101) / 265-VALVYGQMNEPPGAR-279 (71) / 265-VALVYGQMNEPPGAR-279 Oxidation (M) (22) [1].
MAPPING (identification):
SPOT 331: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 507: Peptide mass fingerprinting [1];
SPOT 795: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 875: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP10719; ATPB_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameATPB_RAT
Primary accession numberP10719
Secondary accession number(s) Q499W0
Sequence was last modified on April 1, 1990 (version 2)
Annotations were last modified on November 16, 2011 (version 124)
Name and origin of the protein
DescriptionRecName: Full=ATP synthase subunit beta, mitochondrial; EC=3.6.3.14; Flags: Precursor;
Gene nameName=Atp5b
Encoded onName=Atp5b
Keywords3D-structure; Acetylation; ATP synthesis; ATP-binding; CF(1); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Hydrolase; Ion transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBC099743; AAH99743.1; -; mRNA
EMBLM25301; AAA57154.1; -; mRNA
EMBLM19044; AAB02288.1; ALT_SEQ; mRNA
EMBLM57634; AAA40778.1; -; mRNA
IPIIPI00551812; -; .
PIRA28701; A28701; .
PIRA30160; A30160; .
RefSeqNP_599191.1; NM_134364.1; .
UniGeneRn.92965; -; .
PDB1MAB; X-ray; 2.80 A; B=51-529
PDB2F43; X-ray; 3.00 A; B=51-529
PDBsum1MAB; -; .
PDBsum2F43; -; .
ProteinModelPortalP10719; -; .
SMRP10719; 51-527; .
IntActP10719; 3; .
MINTMINT-4587159; -; .
STRINGP10719; -; .
PhosphoSiteP10719; -; .
UCD-2DPAGEP10719; -; .
World-2DPAGE0004:P10719; -; .
PRIDEP10719; -; .
GeneID171374; -; .
KEGGrno:171374; -; .
UCSCNM_134364; rat; .
CTD506; -; .
RGD621368; Atp5b; .
eggNOGroNOG07745; -; .
HOVERGENHBG004307; -; .
InParanoidP10719; -; .
OrthoDBEOG4ZCT4C; -; .
PhylomeDBP10719; -; .
NextBio622183; -; .
ArrayExpressP10719; -; .
GenevestigatorP10719; -; .
GermOnlineENSRNOG00000002840; Rattus norvegicus; .
GOGO:0000275; C:mitochondrial proton-transporting ATP synthase complex; catalytic core F(1); IDA:RGD
GOGO:0005524; F:ATP binding; IDA:RGD; .
GOGO:0005509; F:calcium ion binding; IDA:RGD; .
GOGO:0046933; F:hydrogen ion transporting ATP synthase activity; rotational mechanism; IEA:InterPro
GOGO:0008553; F:hydrogen-exporting ATPase activity; phosphorylative mechanism; IEA:InterPro
GOGO:0030228; F:lipoprotein particle receptor activity; IDA:RGD; .
GOGO:0006172; P:ADP biosynthetic process; IDA:RGD; .
GOGO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro; .
GOGO:0015986; P:ATP synthesis coupled proton transport; IEA:InterPro; .
InterProIPR020003; ATPase_a/bsu_AS; .
InterProIPR003593; ATPase_AAA+_core; .
InterProIPR005722; ATPase_F1-cplx_bsu; .
InterProIPR018118; ATPase_F1/A1-cplx_a/bsu_N; .
InterProIPR000793; ATPase_F1/V1/A1-cplx_a/bsu_C; .
InterProIPR004100; ATPase_F1/V1/A1-cplx_a/bsu_N; .
InterProIPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd; .
InterProIPR024034; ATPase_F1_bsu/V1_C; .
Gene3DG3DSA:1.10.1140.10; G3DSA:1.10.1140.10; 1; .
KOK02133; -; .
PANTHERPTHR15184:SF8; ATPase_F1_b; 1; .
PfamPF00006; ATP-synt_ab; 1; .
PfamPF00306; ATP-synt_ab_C; 1; .
PfamPF02874; ATP-synt_ab_N; 1; .
SMARTSM00382; AAA; 1; .
SUPFAMSSF47917; ATPase_a/b_C; 1; .
SUPFAMSSF50615; ATPase_a/b_N; 1; .
TIGRFAMsTIGR01039; AtpD; 1; .
PROSITEPS00152; ATPASE_ALPHA_BETA; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server