Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 285:

pI=7.10; Mw=57000

SPOT 286:

pI=6.89; Mw=57000

SPOT 289:

pI=6.90; Mw=55000

MAPPING (identification):

SPOT 285: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 286: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 289: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	KPYM_RAT
Primary accession number	P11980
Secondary accession number(s)	P11981
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 113)
Name and origin of the protein
Description	RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;
Gene name	Name=Pkm2 Synonyms=Pykm
Encoded on	Name=Pkm2; Synonyms=Pykm
Keywords	Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M24359; AAB93666.1; -; Genomic_DNA
EMBL	M24359; AAB93667.1; -; Genomic_DNA
EMBL	M14377; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBL	X15800; CAA33799.1; -; mRNA
IPI	IPI00231929; -; .
IPI	IPI00339197; -; .
PIR	A26186; A26186; .
PIR	B26186; B26186; .
RefSeq	NP_445749.1; NM_053297.1; .
UniGene	Rn.1556; -; .
ProteinModelPortal	P11980; -; .
SMR	P11980; 11-531; .
IntAct	P11980; 1; .
MINT	MINT-4587504; -; .
STRING	P11980; -; .
PhosphoSite	P11980; -; .
World-2DPAGE	0004:P11980; -; .
PRIDE	P11980; -; .
Ensembl	ENSRNOT00000015332; ENSRNOP00000015331; ENSRNOG00000011329; .
Ensembl	ENSRNOT00000015398; ENSRNOP00000015398; ENSRNOG00000011329; .
GeneID	25630; -; .
KEGG	rno:25630; -; .
UCSC	NM_053297; rat; .
CTD	5315; -; .
RGD	3337; Pkm2; .
eggNOG	maNOG12287; -; .
GeneTree	ENSGT00390000008859; -; .
HOVERGEN	HBG000941; -; .
InParanoid	P11980; -; .
OrthoDB	EOG40GCQJ; -; .
PhylomeDB	P11980; -; .
NextBio	607431; -; .
Genevestigator	P11980; -; .
GermOnline	ENSRNOG00000011329; Rattus norvegicus; .
GO	GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GO	GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0000287; F:magnesium ion binding; IEA:InterPro; .
GO	GO:0030955; F:potassium ion binding; IEA:InterPro; .
GO	GO:0005515; F:protein binding; IPI:RGD; .
GO	GO:0004743; F:pyruvate kinase activity; IDA:RGD; .
GO	GO:0006754; P:ATP biosynthetic process; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IDA:RGD; .
GO	GO:0001889; P:liver development; IDA:RGD; .
GO	GO:0031100; P:organ regeneration; IDA:RGD; .
GO	GO:0042866; P:pyruvate biosynthetic process; IDA:RGD; .
GO	GO:0009629; P:response to gravity; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0032868; P:response to insulin stimulus; IEP:RGD; .
GO	GO:0014870; P:response to muscle inactivity; IEP:RGD; .
GO	GO:0007584; P:response to nutrient; IEP:RGD; .
GO	GO:0043403; P:skeletal muscle tissue regeneration; IDA:RGD; .
InterPro	IPR001697; Pyr_Knase; .
InterPro	IPR015813; Pyrv/PenolPyrv_Kinase; .
InterPro	IPR011037; Pyrv_Knase-like_insert_dom; .
InterPro	IPR015794; Pyrv_Knase_a/b; .
InterPro	IPR018209; Pyrv_Knase_AS; .
InterPro	IPR015793; Pyrv_Knase_brl; .
InterPro	IPR015795; Pyrv_Knase_C; .
InterPro	IPR015806; Pyrv_Knase_insert_dom; .
Gene3D	G3DSA:2.40.33.10; PK_B_barrel_like; 1; .
Gene3D	G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; .
Gene3D	G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; .
KO	K00873; -; .
PANTHER	PTHR11817; Pyruvate_kinase; 1; .
Pfam	PF00224; PK; 1; .
Pfam	PF02887; PK_C; 1; .
PRINTS	PR01050; PYRUVTKNASE; .
SUPFAM	SSF50800; PK_B_barrel_like; 1; .
SUPFAM	SSF52935; Pyruvate_kinase; 1; .
SUPFAM	SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMs	TIGR01064; Pyruv_kin; 1; .
PROSITE	PS00110; PYRUVATE_KINASE; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: P11980

World-2DPAGE Repository (0014): P11980

P11980

General information about the entry

Entry name

KPYM_RAT

Primary accession number

P11980

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;.

Gene name

Name=Pkm2
Synonyms=Pykm

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2847:

pI=7.08; Mw=57000

IDENTIFICATION: SPOT 2847: SeqCov=53%. MascotPMF=187 [1].
MAPPING (identification):

SPOT 2847: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	KPYM_RAT
Primary accession number	P11980
Secondary accession number(s)	P11981
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 113)
Name and origin of the protein
Description	RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;
Gene name	Name=Pkm2 Synonyms=Pykm
Encoded on	Name=Pkm2; Synonyms=Pykm
Keywords	Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M24359; AAB93666.1; -; Genomic_DNA
EMBL	M24359; AAB93667.1; -; Genomic_DNA
EMBL	M14377; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBL	X15800; CAA33799.1; -; mRNA
IPI	IPI00231929; -; .
IPI	IPI00339197; -; .
PIR	A26186; A26186; .
PIR	B26186; B26186; .
RefSeq	NP_445749.1; NM_053297.1; .
UniGene	Rn.1556; -; .
ProteinModelPortal	P11980; -; .
SMR	P11980; 11-531; .
IntAct	P11980; 1; .
MINT	MINT-4587504; -; .
STRING	P11980; -; .
PhosphoSite	P11980; -; .
World-2DPAGE	0004:P11980; -; .
PRIDE	P11980; -; .
Ensembl	ENSRNOT00000015332; ENSRNOP00000015331; ENSRNOG00000011329; .
Ensembl	ENSRNOT00000015398; ENSRNOP00000015398; ENSRNOG00000011329; .
GeneID	25630; -; .
KEGG	rno:25630; -; .
UCSC	NM_053297; rat; .
CTD	5315; -; .
RGD	3337; Pkm2; .
eggNOG	maNOG12287; -; .
GeneTree	ENSGT00390000008859; -; .
HOVERGEN	HBG000941; -; .
InParanoid	P11980; -; .
OrthoDB	EOG40GCQJ; -; .
PhylomeDB	P11980; -; .
NextBio	607431; -; .
Genevestigator	P11980; -; .
GermOnline	ENSRNOG00000011329; Rattus norvegicus; .
GO	GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GO	GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0000287; F:magnesium ion binding; IEA:InterPro; .
GO	GO:0030955; F:potassium ion binding; IEA:InterPro; .
GO	GO:0005515; F:protein binding; IPI:RGD; .
GO	GO:0004743; F:pyruvate kinase activity; IDA:RGD; .
GO	GO:0006754; P:ATP biosynthetic process; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IDA:RGD; .
GO	GO:0001889; P:liver development; IDA:RGD; .
GO	GO:0031100; P:organ regeneration; IDA:RGD; .
GO	GO:0042866; P:pyruvate biosynthetic process; IDA:RGD; .
GO	GO:0009629; P:response to gravity; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0032868; P:response to insulin stimulus; IEP:RGD; .
GO	GO:0014870; P:response to muscle inactivity; IEP:RGD; .
GO	GO:0007584; P:response to nutrient; IEP:RGD; .
GO	GO:0043403; P:skeletal muscle tissue regeneration; IDA:RGD; .
InterPro	IPR001697; Pyr_Knase; .
InterPro	IPR015813; Pyrv/PenolPyrv_Kinase; .
InterPro	IPR011037; Pyrv_Knase-like_insert_dom; .
InterPro	IPR015794; Pyrv_Knase_a/b; .
InterPro	IPR018209; Pyrv_Knase_AS; .
InterPro	IPR015793; Pyrv_Knase_brl; .
InterPro	IPR015795; Pyrv_Knase_C; .
InterPro	IPR015806; Pyrv_Knase_insert_dom; .
Gene3D	G3DSA:2.40.33.10; PK_B_barrel_like; 1; .
Gene3D	G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; .
Gene3D	G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; .
KO	K00873; -; .
PANTHER	PTHR11817; Pyruvate_kinase; 1; .
Pfam	PF00224; PK; 1; .
Pfam	PF02887; PK_C; 1; .
PRINTS	PR01050; PYRUVTKNASE; .
SUPFAM	SSF50800; PK_B_barrel_like; 1; .
SUPFAM	SSF52935; Pyruvate_kinase; 1; .
SUPFAM	SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMs	TIGR01064; Pyruv_kin; 1; .
PROSITE	PS00110; PYRUVATE_KINASE; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: P11980

World-2DPAGE Repository (0019): P11980

P11980

General information about the entry

Entry name

KPYM_RAT

Primary accession number

P11980

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;.

Gene name

Name=Pkm2
Synonyms=Pykm

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 380:

pI=6.63; Mw=58294

SPOT 382:

pI=6.63; Mw=58294

SPOT 385:

pI=6.63; Mw=58294

SPOT 388:

pI=6.63; Mw=58294

IDENTIFICATION: SPOT 380: Peptides number=14. SeqCov=31.5%. MOWSE score=3.50E+10 [1]
SPOT 382: Peptides number=11. SeqCov=25.2%. MOWSE score=3.97E+8 [1]; SPOT 385: Peptides number=13. SeqCov=30.3%. MOWSE score=7.43E+11 [1]; SPOT 388: Peptides number=11. SeqCov=23.5%. MOWSE score=8.35E+7 [1].
MAPPING (identification):

SPOT 380: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 382: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 385: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 388: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	KPYM_RAT
Primary accession number	P11980
Secondary accession number(s)	P11981
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 113)
Name and origin of the protein
Description	RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;
Gene name	Name=Pkm2 Synonyms=Pykm
Encoded on	Name=Pkm2; Synonyms=Pykm
Keywords	Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M24359; AAB93666.1; -; Genomic_DNA
EMBL	M24359; AAB93667.1; -; Genomic_DNA
EMBL	M14377; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBL	X15800; CAA33799.1; -; mRNA
IPI	IPI00231929; -; .
IPI	IPI00339197; -; .
PIR	A26186; A26186; .
PIR	B26186; B26186; .
RefSeq	NP_445749.1; NM_053297.1; .
UniGene	Rn.1556; -; .
ProteinModelPortal	P11980; -; .
SMR	P11980; 11-531; .
IntAct	P11980; 1; .
MINT	MINT-4587504; -; .
STRING	P11980; -; .
PhosphoSite	P11980; -; .
World-2DPAGE	0004:P11980; -; .
PRIDE	P11980; -; .
Ensembl	ENSRNOT00000015332; ENSRNOP00000015331; ENSRNOG00000011329; .
Ensembl	ENSRNOT00000015398; ENSRNOP00000015398; ENSRNOG00000011329; .
GeneID	25630; -; .
KEGG	rno:25630; -; .
UCSC	NM_053297; rat; .
CTD	5315; -; .
RGD	3337; Pkm2; .
eggNOG	maNOG12287; -; .
GeneTree	ENSGT00390000008859; -; .
HOVERGEN	HBG000941; -; .
InParanoid	P11980; -; .
OrthoDB	EOG40GCQJ; -; .
PhylomeDB	P11980; -; .
NextBio	607431; -; .
Genevestigator	P11980; -; .
GermOnline	ENSRNOG00000011329; Rattus norvegicus; .
GO	GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GO	GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0000287; F:magnesium ion binding; IEA:InterPro; .
GO	GO:0030955; F:potassium ion binding; IEA:InterPro; .
GO	GO:0005515; F:protein binding; IPI:RGD; .
GO	GO:0004743; F:pyruvate kinase activity; IDA:RGD; .
GO	GO:0006754; P:ATP biosynthetic process; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IDA:RGD; .
GO	GO:0001889; P:liver development; IDA:RGD; .
GO	GO:0031100; P:organ regeneration; IDA:RGD; .
GO	GO:0042866; P:pyruvate biosynthetic process; IDA:RGD; .
GO	GO:0009629; P:response to gravity; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0032868; P:response to insulin stimulus; IEP:RGD; .
GO	GO:0014870; P:response to muscle inactivity; IEP:RGD; .
GO	GO:0007584; P:response to nutrient; IEP:RGD; .
GO	GO:0043403; P:skeletal muscle tissue regeneration; IDA:RGD; .
InterPro	IPR001697; Pyr_Knase; .
InterPro	IPR015813; Pyrv/PenolPyrv_Kinase; .
InterPro	IPR011037; Pyrv_Knase-like_insert_dom; .
InterPro	IPR015794; Pyrv_Knase_a/b; .
InterPro	IPR018209; Pyrv_Knase_AS; .
InterPro	IPR015793; Pyrv_Knase_brl; .
InterPro	IPR015795; Pyrv_Knase_C; .
InterPro	IPR015806; Pyrv_Knase_insert_dom; .
Gene3D	G3DSA:2.40.33.10; PK_B_barrel_like; 1; .
Gene3D	G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; .
Gene3D	G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; .
KO	K00873; -; .
PANTHER	PTHR11817; Pyruvate_kinase; 1; .
Pfam	PF00224; PK; 1; .
Pfam	PF02887; PK_C; 1; .
PRINTS	PR01050; PYRUVTKNASE; .
SUPFAM	SSF50800; PK_B_barrel_like; 1; .
SUPFAM	SSF52935; Pyruvate_kinase; 1; .
SUPFAM	SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMs	TIGR01064; Pyruv_kin; 1; .
PROSITE	PS00110; PYRUVATE_KINASE; 1; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: P11980

World-2DPAGE Repository (0022): P11980

P11980

General information about the entry

Entry name

KPYM_RAT

Primary accession number

P11980

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;.

Gene name

Name=Pkm2
Synonyms=Pykm

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 408:

pI=6.63; Mw=58294

SPOT 957:

pI=6.63; Mw=58294

IDENTIFICATION: SPOT 408: SeqCov=14.7%. Peptides MS=9/20. Mascot PMF Score=43 [1]
SPOT 957: SeqCov=25.8%. Peptides MS=13/20. Mascot PMF Score=104 [1].
PEPTIDE EVIDENCE: SPOT 408: 448-APIIAVTR-455 (27) / 393-LLFEELAR-400 (36) [1]
SPOT 957: 57-SVEMLK-62 Oxidation (M) (5) / 393-LLFEELAR-400 (23) / 93-AATESFASDPILYRPVAVALDTK-115 (17) [1].
MAPPING (identification):

SPOT 408: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 957: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	KPYM_RAT
Primary accession number	P11980
Secondary accession number(s)	P11981
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 113)
Name and origin of the protein
Description	RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;
Gene name	Name=Pkm2 Synonyms=Pykm
Encoded on	Name=Pkm2; Synonyms=Pykm
Keywords	Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M24359; AAB93666.1; -; Genomic_DNA
EMBL	M24359; AAB93667.1; -; Genomic_DNA
EMBL	M14377; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBL	X15800; CAA33799.1; -; mRNA
IPI	IPI00231929; -; .
IPI	IPI00339197; -; .
PIR	A26186; A26186; .
PIR	B26186; B26186; .
RefSeq	NP_445749.1; NM_053297.1; .
UniGene	Rn.1556; -; .
ProteinModelPortal	P11980; -; .
SMR	P11980; 11-531; .
IntAct	P11980; 1; .
MINT	MINT-4587504; -; .
STRING	P11980; -; .
PhosphoSite	P11980; -; .
World-2DPAGE	0004:P11980; -; .
PRIDE	P11980; -; .
Ensembl	ENSRNOT00000015332; ENSRNOP00000015331; ENSRNOG00000011329; .
Ensembl	ENSRNOT00000015398; ENSRNOP00000015398; ENSRNOG00000011329; .
GeneID	25630; -; .
KEGG	rno:25630; -; .
UCSC	NM_053297; rat; .
CTD	5315; -; .
RGD	3337; Pkm2; .
eggNOG	maNOG12287; -; .
GeneTree	ENSGT00390000008859; -; .
HOVERGEN	HBG000941; -; .
InParanoid	P11980; -; .
OrthoDB	EOG40GCQJ; -; .
PhylomeDB	P11980; -; .
NextBio	607431; -; .
Genevestigator	P11980; -; .
GermOnline	ENSRNOG00000011329; Rattus norvegicus; .
GO	GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GO	GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0043531; F:ADP binding; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0000287; F:magnesium ion binding; IEA:InterPro; .
GO	GO:0030955; F:potassium ion binding; IEA:InterPro; .
GO	GO:0005515; F:protein binding; IPI:RGD; .
GO	GO:0004743; F:pyruvate kinase activity; IDA:RGD; .
GO	GO:0006754; P:ATP biosynthetic process; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IDA:RGD; .
GO	GO:0001889; P:liver development; IDA:RGD; .
GO	GO:0031100; P:organ regeneration; IDA:RGD; .
GO	GO:0042866; P:pyruvate biosynthetic process; IDA:RGD; .
GO	GO:0009629; P:response to gravity; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0032868; P:response to insulin stimulus; IEP:RGD; .
GO	GO:0014870; P:response to muscle inactivity; IEP:RGD; .
GO	GO:0007584; P:response to nutrient; IEP:RGD; .
GO	GO:0043403; P:skeletal muscle tissue regeneration; IDA:RGD; .
InterPro	IPR001697; Pyr_Knase; .
InterPro	IPR015813; Pyrv/PenolPyrv_Kinase; .
InterPro	IPR011037; Pyrv_Knase-like_insert_dom; .
InterPro	IPR015794; Pyrv_Knase_a/b; .
InterPro	IPR018209; Pyrv_Knase_AS; .
InterPro	IPR015793; Pyrv_Knase_brl; .
InterPro	IPR015795; Pyrv_Knase_C; .
InterPro	IPR015806; Pyrv_Knase_insert_dom; .
Gene3D	G3DSA:2.40.33.10; PK_B_barrel_like; 1; .
Gene3D	G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; .
Gene3D	G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; .
KO	K00873; -; .
PANTHER	PTHR11817; Pyruvate_kinase; 1; .
Pfam	PF00224; PK; 1; .
Pfam	PF02887; PK_C; 1; .
PRINTS	PR01050; PYRUVTKNASE; .
SUPFAM	SSF50800; PK_B_barrel_like; 1; .
SUPFAM	SSF52935; Pyruvate_kinase; 1; .
SUPFAM	SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMs	TIGR01064; Pyruv_kin; 1; .
PROSITE	PS00110; PYRUVATE_KINASE; 1; .

Searching in 'World-2DPAGE Repository [0030]' for entry matching: P11980

World-2DPAGE Repository (0030): P11980

P11980

General information about the entry

Entry name

KPYM_RAT

Primary accession number

P11980

integrated into World-2DPAGE Repository (0030) on

November 9, 2010 (release 1)

2D Annotations were last modified on

June 21, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;.

Gene name

Name=Pkm2
Synonyms=Pykm

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011)

2D PAGE maps for identified proteins

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)

RAT_INS1E_4-7

MAP LOCATIONS:

SPOT 812:

pI=6.77; Mw=68969

SPOT 856:

pI=6.77; Mw=67087

SPOT 1603:

pI=5.04; Mw=42675

SPOT 1651:

pI=5.00; Mw=41799

IDENTIFICATION: SPOT 812: Mascot protein score=211. Number of peptides=4 [1]
SPOT 856: Mascot protein score=137. Number of peptides=5 [1]; SPOT 1603: Mascot protein score=265. Number of peptides=5 [1]; SPOT 1651: Mascot protein score=281. Number of peptides=7 [1].
MAPPING (identification):

SPOT 812: Tandem mass spectrometry [1];
SPOT 856: Tandem mass spectrometry [1];
SPOT 1603: Tandem mass spectrometry [1];
SPOT 1651: Tandem mass spectrometry [1].

Copyright

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium

Cross-references

UniProtKB/Swiss-Prot

World-2DPAGE Repository